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The map label for this gene is amiD [H]

Identifier: 77456277

GI number: 77456277

Start: 54274

End: 55053

Strand: Reverse

Name: amiD [H]

Synonym: Pfl01_0049

Alternate gene names: 77456277

Gene position: 55053-54274 (Counterclockwise)

Preceding gene: 77456278

Following gene: 77456276

Centisome position: 0.86

GC content: 61.79

Gene sequence:

>780_bases
ATGAAATTTTTTACCCTCCTCGCGTCGCTCCTCTTACTGGCCGGTTGTGCCAGCGGCCCGCGTTTTGACACCAGCCATCC
TTCGGTCAATCACGACAGTCGCGTGCAGTTTGTGATCGTGCACTACACCTCCGCCTCGCTTGAACGCTCACTGCAGTTGC
TGACCCACGGCGAAGTCAGCAGTCATTACCTGATCGGCGACGACAAGAGCGCCACCATCTACAAGCTGATGGACGAAAAC
CTGCGGGCCTGGCATGCCGGAGAAAGCGAATGGCAAGGCCGGACCTGGCTCAACTCCAGCTCGATCGGTATCGAGATCGT
CAATCCGGGCTTCAAGGACACACCGACCGGTCGCCTCTGGTATCCCTACAGCGAAGCCCAGGTGCAATCGCTCATCGTTC
TGCTCAAGGACATCAGCAAACGCCAGGGCATCAGCCCGCGCCACATCATCGGCCATAGCGACATCGCCCCGTTGCGCAAG
CTCGACCCGGGGCCGCTGTTCCCCTGGAAGCGCCTGGCAGCCGAAGGGTTGGGGCTCTGGCCCAATGAACAAGCGGTGGC
ACGCCAACAGGCGCAGTTCGACGCCGAACTGCCGAGCATCACCTGGTTCCAGAGCCAACTGGCGCGCCTGGGCTACTCGA
CGCCGCAGACCGGCGAGCTGGATGTGGCGACCCGGCACGTGCTCGCGGCCTTCCAGATGCACTACCGCCCTGCGCGCTTT
GACGGCACGCCGGATGCGCAAACGGCAGCGTTGCTGCTGGTGCTGAATCAGACAAAATAA

Upstream 100 bases:

>100_bases
GCAATTGTTCCATATTGTTTAAATCAGCCCTTTAAGCGTCGAAGCGCAAGGCGATACGTTACACTGTTGCATTATCGTCT
TGCGTGTACTGCCCTCTGCC

Downstream 100 bases:

>100_bases
TGACGCCCGCCCGACGGTCAGGGCGAAAATGCAATCAGCAGCTATAACTCATTGGTAATTCTTCGGATATCCATGATGGT
TGCTACCCGAGAGACGCTGC

Product: AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKFFTLLASLLLLAGCASGPRFDTSHPSVNHDSRVQFVIVHYTSASLERSLQLLTHGEVSSHYLIGDDKSATIYKLMDEN
LRAWHAGESEWQGRTWLNSSSIGIEIVNPGFKDTPTGRLWYPYSEAQVQSLIVLLKDISKRQGISPRHIIGHSDIAPLRK
LDPGPLFPWKRLAAEGLGLWPNEQAVARQQAQFDAELPSITWFQSQLARLGYSTPQTGELDVATRHVLAAFQMHYRPARF
DGTPDAQTAALLLVLNQTK

Sequences:

>Translated_259_residues
MKFFTLLASLLLLAGCASGPRFDTSHPSVNHDSRVQFVIVHYTSASLERSLQLLTHGEVSSHYLIGDDKSATIYKLMDEN
LRAWHAGESEWQGRTWLNSSSIGIEIVNPGFKDTPTGRLWYPYSEAQVQSLIVLLKDISKRQGISPRHIIGHSDIAPLRK
LDPGPLFPWKRLAAEGLGLWPNEQAVARQQAQFDAELPSITWFQSQLARLGYSTPQTGELDVATRHVLAAFQMHYRPARF
DGTPDAQTAALLLVLNQTK
>Mature_259_residues
MKFFTLLASLLLLAGCASGPRFDTSHPSVNHDSRVQFVIVHYTSASLERSLQLLTHGEVSSHYLIGDDKSATIYKLMDEN
LRAWHAGESEWQGRTWLNSSSIGIEIVNPGFKDTPTGRLWYPYSEAQVQSLIVLLKDISKRQGISPRHIIGHSDIAPLRK
LDPGPLFPWKRLAAEGLGLWPNEQAVARQQAQFDAELPSITWFQSQLARLGYSTPQTGELDVATRHVLAAFQMHYRPARF
DGTPDAQTAALLLVLNQTK

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=268, Percent_Identity=40.2985074626866, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1786300, Length=119, Percent_Identity=38.655462184874, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28927; Mature: 28927

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFTLLASLLLLAGCASGPRFDTSHPSVNHDSRVQFVIVHYTSASLERSLQLLTHGEVS
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCC
SHYLIGDDKSATIYKLMDENLRAWHAGESEWQGRTWLNSSSIGIEIVNPGFKDTPTGRLW
CEEEECCCCCCHHHHHHHHCHHEEECCCCCCCCCEEECCCCCEEEEECCCCCCCCCCEEE
YPYSEAQVQSLIVLLKDISKRQGISPRHIIGHSDIAPLRKLDPGPLFPWKRLAAEGLGLW
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHCCCCCC
PNEQAVARQQAQFDAELPSITWFQSQLARLGYSTPQTGELDVATRHVLAAFQMHYRPARF
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
DGTPDAQTAALLLVLNQTK
CCCCCHHHEEEEEEEECCC
>Mature Secondary Structure
MKFFTLLASLLLLAGCASGPRFDTSHPSVNHDSRVQFVIVHYTSASLERSLQLLTHGEVS
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCC
SHYLIGDDKSATIYKLMDENLRAWHAGESEWQGRTWLNSSSIGIEIVNPGFKDTPTGRLW
CEEEECCCCCCHHHHHHHHCHHEEECCCCCCCCCEEECCCCCEEEEECCCCCCCCCCEEE
YPYSEAQVQSLIVLLKDISKRQGISPRHIIGHSDIAPLRKLDPGPLFPWKRLAAEGLGLW
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCCCHHHHHHHCCCCCC
PNEQAVARQQAQFDAELPSITWFQSQLARLGYSTPQTGELDVATRHVLAAFQMHYRPARF
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
DGTPDAQTAALLLVLNQTK
CCCCCHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]