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The map label for this gene is rsmB [H]

Identifier: 77456243

GI number: 77456243

Start: 17178

End: 18488

Strand: Reverse

Name: rsmB [H]

Synonym: Pfl01_0015

Alternate gene names: 77456243

Gene position: 18488-17178 (Counterclockwise)

Preceding gene: 77456244

Following gene: 77456242

Centisome position: 0.29

GC content: 64.91

Gene sequence:

>1311_bases
ATGAACCCGCGTCTGGCCGCCGCCAAGGCACTCGCCGCTGTTCTCAGTGGCAAGGCTTCGCTCAACAGCTCCCTGCCGAC
GCAACTGGACAAGGTCGAAGATCGCGATCGCGGTTTCACCCAGGACCTGGCGTTCGGCACCGCCCGTTGGCAGCCACGTT
TGTCGGCGCTGGCGGAAAAACTGCTGCAGAAGCCGTTCAAGGCAGCGGACGCCGATGTCGAGGCATTGCTGCTGGTCGGT
CTCTATCAACTGCTCTACACCCGCGTCCCACCGCACGCCGCCATCGGCGAAACCGTCGGTTGCGCCGACAAGCTGAAAAA
GCCGTGGGCCAAAGGCTTGCTCAACGCCGTACTGCGCAATGCCCAGCGCGAACATGAGGCGATCTTCGCCGAACTGGAGC
GCGATCCGGTGGTGCGTACTGCCCACCCGCGCTGGCTGCAAAAATCCCTCAAGGCCTTCTGGCCTGAGCAATGGGAAGCG
ATTTGCGAGGCCAACAACGCGCATCCGCCGATGATCCTGCGGGTCAACCGTCGTCATCACAGCCGTGACGCGTACCTGGG
TCTGCTGACTGAAGCCGGTATCGCCGCGCAGCCGTGCACTTTCAGCCGCGACGGTATCGTGCTGGAGGCCGCCGCCGACG
TGCGCAGCCTGCCGGGCTTCGCCGAAGGCTGGATCAGCGTGCAAGACGAAGCCGCACAACTGGCCGCCGACCTGCTCGAC
CTCGCGCCGGGCCAGCGAGTGCTGGACGCCTGCTGTGCACCGGGCGGCAAGACGTGCCACATCCTCGAAGCCGAACCGGC
ACTGGCCGGCGTGGTGGCGGTGGATCTGGAAGCCAAACGTCTGGTGCGAGTGAAGGAAAACCTCGAGCGTCTCGGTCTGA
ACGCCGAACTGATCGCTGCCGATGGTCGCGACACGGCAGCCTGGTGGGACGGCAAACCGTTCCAGCGCATCCTGCTCGAC
GCGCCGTGCTCGGCCACCGGAGTAATCCGCCGTCACCCGGACATCAAGCTGACCCGTCAGGCCGACGACATCGTTGCCCT
CGCGCAACTGCAAGGCGAACTGCTCGACGCGATGTGGAAAACCCTCGAAGTGGGCGGCATCCTGCTCTACGCCACCTGCT
CGACCTTGCCGACCGAAAATACCGAAGTCATCGGCGCGTTTCTTGAGCGCACGCCGGGCGCCCGGGAGCTGGATCTGGCT
ACCACCGCCGGGATCAAGCAGCCTCATGGTCGCCAATTGCTGGCCCAACAGGGCGGGCATGACGGGTTCTACTACGCCAA
GCTGATCAAGATCGCTGCCGCGCGCGGCTAA

Upstream 100 bases:

>100_bases
AATTGCCCGGCGGCAAGGCGCTGAACTTCAGCGATCTGTTCAACAGCCGTCGTGAGAAATTCGCCACCGGCATCGTGCTG
GGTGCAGCGGTGGACGCTCA

Downstream 100 bases:

>100_bases
ACGGATTCAACGGAGTGACTGGATGAAAATCATCATCCTCGGTGCGGGACAGGTCGGCGGTTCGCTGGCTGAACATCTGG
CCAGCGAGGCCAACGACATC

Product: Fmu, rRNA SAM-dependent methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAEKLLQKPFKAADADVEALLLVG
LYQLLYTRVPPHAAIGETVGCADKLKKPWAKGLLNAVLRNAQREHEAIFAELERDPVVRTAHPRWLQKSLKAFWPEQWEA
ICEANNAHPPMILRVNRRHHSRDAYLGLLTEAGIAAQPCTFSRDGIVLEAAADVRSLPGFAEGWISVQDEAAQLAADLLD
LAPGQRVLDACCAPGGKTCHILEAEPALAGVVAVDLEAKRLVRVKENLERLGLNAELIAADGRDTAAWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIVALAQLQGELLDAMWKTLEVGGILLYATCSTLPTENTEVIGAFLERTPGARELDLA
TTAGIKQPHGRQLLAQQGGHDGFYYAKLIKIAAARG

Sequences:

>Translated_436_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAEKLLQKPFKAADADVEALLLVG
LYQLLYTRVPPHAAIGETVGCADKLKKPWAKGLLNAVLRNAQREHEAIFAELERDPVVRTAHPRWLQKSLKAFWPEQWEA
ICEANNAHPPMILRVNRRHHSRDAYLGLLTEAGIAAQPCTFSRDGIVLEAAADVRSLPGFAEGWISVQDEAAQLAADLLD
LAPGQRVLDACCAPGGKTCHILEAEPALAGVVAVDLEAKRLVRVKENLERLGLNAELIAADGRDTAAWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIVALAQLQGELLDAMWKTLEVGGILLYATCSTLPTENTEVIGAFLERTPGARELDLA
TTAGIKQPHGRQLLAQQGGHDGFYYAKLIKIAAARG
>Mature_436_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAEKLLQKPFKAADADVEALLLVG
LYQLLYTRVPPHAAIGETVGCADKLKKPWAKGLLNAVLRNAQREHEAIFAELERDPVVRTAHPRWLQKSLKAFWPEQWEA
ICEANNAHPPMILRVNRRHHSRDAYLGLLTEAGIAAQPCTFSRDGIVLEAAADVRSLPGFAEGWISVQDEAAQLAADLLD
LAPGQRVLDACCAPGGKTCHILEAEPALAGVVAVDLEAKRLVRVKENLERLGLNAELIAADGRDTAAWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIVALAQLQGELLDAMWKTLEVGGILLYATCSTLPTENTEVIGAFLERTPGARELDLA
TTAGIKQPHGRQLLAQQGGHDGFYYAKLIKIAAARG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=351, Percent_Identity=30.4843304843305, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI76150623, Length=351, Percent_Identity=30.4843304843305, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI32698918, Length=181, Percent_Identity=32.0441988950276, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI23199998, Length=198, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI270288816, Length=198, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI270288818, Length=198, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI8922322, Length=198, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI301336155, Length=195, Percent_Identity=31.2820512820513, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI39995082, Length=195, Percent_Identity=31.2820512820513, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI2367212, Length=429, Percent_Identity=50.5827505827506, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI87081985, Length=276, Percent_Identity=31.1594202898551, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=341, Percent_Identity=28.7390029325513, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25143471, Length=196, Percent_Identity=30.1020408163265, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=312, Percent_Identity=28.2051282051282, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319447, Length=186, Percent_Identity=30.6451612903226, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI22024126, Length=336, Percent_Identity=27.9761904761905, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355201, Length=194, Percent_Identity=31.9587628865979, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356513, Length=197, Percent_Identity=32.48730964467, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24668781, Length=197, Percent_Identity=32.48730964467, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=176, Percent_Identity=26.7045454545455, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47375; Mature: 47375

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAEK
CCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHH
LLQKPFKAADADVEALLLVGLYQLLYTRVPPHAAIGETVGCADKLKKPWAKGLLNAVLRN
HHHCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHHH
AQREHEAIFAELERDPVVRTAHPRWLQKSLKAFWPEQWEAICEANNAHPPMILRVNRRHH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCC
SRDAYLGLLTEAGIAAQPCTFSRDGIVLEAAADVRSLPGFAEGWISVQDEAAQLAADLLD
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHCCCHHHCCEECHHHHHHHHHHHHH
LAPGQRVLDACCAPGGKTCHILEAEPALAGVVAVDLEAKRLVRVKENLERLGLNAELIAA
CCCCHHHHHHHCCCCCCEEEEEECCCCHHEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEE
DGRDTAAWWDGKPFQRILLDAPCSATGVIRRHPDIKLTRQADDIVALAQLQGELLDAMWK
CCCCCCCCCCCCHHHHHHHCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHH
TLEVGGILLYATCSTLPTENTEVIGAFLERTPGARELDLATTAGIKQPHGRQLLAQQGGH
HHHHCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEECHHHHCCCCCCCCHHHHHHCCCC
DGFYYAKLIKIAAARG
CCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAEK
CCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHH
LLQKPFKAADADVEALLLVGLYQLLYTRVPPHAAIGETVGCADKLKKPWAKGLLNAVLRN
HHHCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHHH
AQREHEAIFAELERDPVVRTAHPRWLQKSLKAFWPEQWEAICEANNAHPPMILRVNRRHH
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEECCCCC
SRDAYLGLLTEAGIAAQPCTFSRDGIVLEAAADVRSLPGFAEGWISVQDEAAQLAADLLD
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHCCCHHHCCEECHHHHHHHHHHHHH
LAPGQRVLDACCAPGGKTCHILEAEPALAGVVAVDLEAKRLVRVKENLERLGLNAELIAA
CCCCHHHHHHHCCCCCCEEEEEECCCCHHEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEE
DGRDTAAWWDGKPFQRILLDAPCSATGVIRRHPDIKLTRQADDIVALAQLQGELLDAMWK
CCCCCCCCCCCCHHHHHHHCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHH
TLEVGGILLYATCSTLPTENTEVIGAFLERTPGARELDLATTAGIKQPHGRQLLAQQGGH
HHHHCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEECHHHHCCCCCCCCHHHHHHCCCC
DGFYYAKLIKIAAARG
CCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]