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The map label for this gene is imm [H]

Identifier: 255961283

GI number: 255961283

Start: 6114787

End: 6115062

Strand: Direct

Name: imm [H]

Synonym: Pfl01_5448

Alternate gene names: 255961283

Gene position: 6114787-6115062 (Clockwise)

Preceding gene: 77461666

Following gene: 77461672

Centisome position: 94.97

GC content: 46.01

Gene sequence:

>276_bases
ATGAAATTAAAAGACAAAATCGAAGATTATACCGAAACTGAATTTCTGCTTTTCGTAACGGAGTTTTTCGAGAATACAAA
CAGCCTGACCGGCGACGCACTAAGCGCATACACAAGTAATCTCGCACTTCATTTTGAGAAAATAACCGAGCACCCGAGAA
AGCGCGCGGTAATTTTCCGACCGACACCCGACCGTGAGGACAGTGCCGCTGGAGTAGTAGAAGAAATCAAAAAATGGCGC
CAGGCAAACGGCAAGCCCGGATTCAAGGCTGAATAA

Upstream 100 bases:

>100_bases
TACATCACATCAAAGATGGTGGCGAAGTTTACAATATTGACAACTTAACCATAATCACCCCTCGCGCACATATAGAACTA
CACAAGAATGGTATAGAACC

Downstream 100 bases:

>100_bases
TTCATAAATCAGACAGTGTCACACCTACAGCGACACTGTCTGGCTTTTTACTAAAGCAATTCGCGAATATCCTCCGGCAA
CAAATTCACATACCGACTAT

Product: colicin/pyocin immunity family protein

Products: NA

Alternate protein names: ImmE7; Microcin-E7 immunity protein [H]

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MKLKDKIEDYTETEFLLFVTEFFENTNSLTGDALSAYTSNLALHFEKITEHPRKRAVIFRPTPDREDSAAGVVEEIKKWR
QANGKPGFKAE

Sequences:

>Translated_91_residues
MKLKDKIEDYTETEFLLFVTEFFENTNSLTGDALSAYTSNLALHFEKITEHPRKRAVIFRPTPDREDSAAGVVEEIKKWR
QANGKPGFKAE
>Mature_91_residues
MKLKDKIEDYTETEFLLFVTEFFENTNSLTGDALSAYTSNLALHFEKITEHPRKRAVIFRPTPDREDSAAGVVEEIKKWR
QANGKPGFKAE

Specific function: This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleave

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicins ColE2/ColE8/ColE9 and pyocins S1/S2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000290
- ProDom:   PD007225 [H]

Pfam domain/function: PF01320 Colicin_Pyocin [H]

EC number: NA

Molecular weight: Translated: 10465; Mature: 10465

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKDKIEDYTETEFLLFVTEFFENTNSLTGDALSAYTSNLALHFEKITEHPRKRAVIFR
CCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCEEEEC
PTPDREDSAAGVVEEIKKWRQANGKPGFKAE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLKDKIEDYTETEFLLFVTEFFENTNSLTGDALSAYTSNLALHFEKITEHPRKRAVIFR
CCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCEEEEC
PTPDREDSAAGVVEEIKKWRQANGKPGFKAE
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2045785; 8692833; 9135159; 10368275 [H]