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The map label for this gene is anfA [H]

Identifier: 76819836

GI number: 76819836

Start: 1688682

End: 1689938

Strand: Direct

Name: anfA [H]

Synonym: BURPS1710b_A1383

Alternate gene names: 76819836

Gene position: 1688682-1689938 (Clockwise)

Preceding gene: 76818706

Following gene: 76818037

Centisome position: 53.07

GC content: 64.04

Gene sequence:

>1257_bases
ATGCGATCGAAGTTCGATCGCGGCGACGAAACGCGTTGCTCATCACAGTATAGAAAACGCGCCCTCGGCGAAACCCGGTT
ATCGGATATTTCTATCCGAACCCGGCCGCCGCATTGGAAATTTCAATTCATCGTTGAAAAAATAGCCGCTAGGCTTCAGC
TGTGTAAAAAATGCGCAGGGCGCATCCGAACGCCGTTCGTTCGCACGCTTGTTTGCTCGCTCATTCGCTCGTTCGCTTCG
GAGCCAGTCCGCGCACCTCCCCGGGGAACCCCACTCAGCGAGATACCCATGTCGCAACACAGCAGTGACGGCAGACGGCT
GCACGGCAATTCGTGTCTGATCCAGGCGCTGTTGCACAAGATCGAAAAAGTGGCCGCGACTCGTGCAAGCGTGTTGATCG
TCGGCGAAAGCGGGGTCGGCAAAGATATCGTCGCAAGACTCCTCCATGACATGAGCCCACGCCGCCGTGGCCCGTTCGTT
CCGGTCAATTGCGGCGCGATTCCGGCCGACATCGCCGAATCGCAGTTGTTCGGCCACGAGAAGGGCAGCTTCACCGGCGC
GGCCGCGCAGCACATCGGCTTCTTCGAGGCCGCGCGCGGCGGCACGATCTTCCTCGACGAAGTGGCCGAGATGTCGCGCG
ATCTGCAGGTGAAGCTGCTGCGCGCGCTCGAATCGAACACCATCACGCGCGTGGGCGGCAGCGAGCCGATCGCGCTCGAC
GTGCGCGTGGTCGCGGCCACTCACCACAACCCTGCCGAGGCGGTGCAGGAAGGGCGCTTTCGCGAAGACCTGTTCTACCG
GCTCGCCGTGTTCGCGCTGCGCGTGCCGGCGCTCAGGCATCGCGAGGACGATATCGAGGGCATCGCGCAGGAGCTCGTCG
ATACGCTCAACAGCCGGCACAGCACGCGCAAGCGGCTCTCGGGGCAGGCGCTGAAGACGCTGCGCACCTATTCGTGGCCG
GGCAACGTGCGCGAGCTGCGCAATGCGATCGAGCGCGCGTACATCCTCTCCGACGAGCAGATCGAGCAGTTGCCGGTGCG
ACGGCTCGCGCCGCGCGACGACGTGCGCCGGAACGCGATGACGCTGCCGCTCGGCATGACGCTCGCGCATTCGCAGCAGC
GCTTCATCGCCGCGTCGCTCAAATACTTCGACGGCGACAAGCCGCGCACCGCGAAGGCGCTCGGCATCAGCCTGAAGACG
CTGTACAACCGGCTCGCGCTGATGCGCGAGCACGACGGGGAAACCGCGCAGCACTAA

Upstream 100 bases:

>100_bases
TCGAGCTCCTTCGTCCGTTGCGCGCCGGCGCAAGCGATGAACGAGCCCGCATGCCGGCGCATATCCGATTAGGCGGCCGA
ACGATGCCGGCCGATGTGCG

Downstream 100 bases:

>100_bases
GGGGGGCGGACGCGCGGCGGGCGAGCCGCATCCGGCCGGCGCGCCTCGCGCGGTGAACGGCTGGCGCTCGTCGTGGCTGT
CGTGGCTGTCGCTCATGTCG

Product: putative sigma-54 interacting transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MRSKFDRGDETRCSSQYRKRALGETRLSDISIRTRPPHWKFQFIVEKIAARLQLCKKCAGRIRTPFVRTLVCSLIRSFAS
EPVRAPPRGTPLSEIPMSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFV
PVNCGAIPADIAESQLFGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALD
VRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWP
GNVRELRNAIERAYILSDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKT
LYNRLALMREHDGETAQH

Sequences:

>Translated_418_residues
MRSKFDRGDETRCSSQYRKRALGETRLSDISIRTRPPHWKFQFIVEKIAARLQLCKKCAGRIRTPFVRTLVCSLIRSFAS
EPVRAPPRGTPLSEIPMSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFV
PVNCGAIPADIAESQLFGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALD
VRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWP
GNVRELRNAIERAYILSDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKT
LYNRLALMREHDGETAQH
>Mature_418_residues
MRSKFDRGDETRCSSQYRKRALGETRLSDISIRTRPPHWKFQFIVEKIAARLQLCKKCAGRIRTPFVRTLVCSLIRSFAS
EPVRAPPRGTPLSEIPMSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFV
PVNCGAIPADIAESQLFGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALD
VRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWP
GNVRELRNAIERAYILSDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKT
LYNRLALMREHDGETAQH

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=325, Percent_Identity=41.8461538461538, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082117, Length=226, Percent_Identity=48.2300884955752, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1788550, Length=305, Percent_Identity=41.3114754098361, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=45.4166666666667, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=39.2405063291139, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=41.2698412698413, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082152, Length=253, Percent_Identity=46.2450592885375, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1789087, Length=239, Percent_Identity=43.0962343096234, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI1790299, Length=241, Percent_Identity=43.9834024896266, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=37.3831775700935, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1787583, Length=303, Percent_Identity=37.953795379538, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=37.1681415929204, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87081858, Length=307, Percent_Identity=30.2931596091205, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 46827; Mature: 46827

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKFDRGDETRCSSQYRKRALGETRLSDISIRTRPPHWKFQFIVEKIAARLQLCKKCAG
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RIRTPFVRTLVCSLIRSFASEPVRAPPRGTPLSEIPMSQHSSDGRRLHGNSCLIQALLHK
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCEECCCHHHHHHHHHH
IEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFVPVNCGAIPADIAESQLFGHE
HHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCC
KGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALD
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEE
VRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRH
EEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
STRKRLSGQALKTLRTYSWPGNVRELRNAIERAYILSDEQIEQLPVRRLAPRDDVRRNAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHCCCHHHHHHCCE
TLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKTLYNRLALMREHDGETAQH
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRSKFDRGDETRCSSQYRKRALGETRLSDISIRTRPPHWKFQFIVEKIAARLQLCKKCAG
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RIRTPFVRTLVCSLIRSFASEPVRAPPRGTPLSEIPMSQHSSDGRRLHGNSCLIQALLHK
HCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCEECCCHHHHHHHHHH
IEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFVPVNCGAIPADIAESQLFGHE
HHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCC
KGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALD
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEE
VRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRH
EEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
STRKRLSGQALKTLRTYSWPGNVRELRNAIERAYILSDEQIEQLPVRRLAPRDDVRRNAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHCCCHHHHHHCCE
TLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKTLYNRLALMREHDGETAQH
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]