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The map label for this gene is potD [C]

Identifier: 76819832

GI number: 76819832

Start: 2175350

End: 2176438

Strand: Direct

Name: potD [C]

Synonym: BURPS1710b_A1786

Alternate gene names: 76819832

Gene position: 2175350-2176438 (Clockwise)

Preceding gene: 76819274

Following gene: 76817642

Centisome position: 68.37

GC content: 67.58

Gene sequence:

>1089_bases
ATGTCTGAAAACCGGCCATTGCTGACGGCGCTGCGCCGCGCCGCGCTCGCCTTCGGCATGTGCGCGACGCTCGTCGCGAA
CGGCGCATCCGCCGAGCCGCTTTACGCGGGCGAAGACGCGCTCTATGCGAAGGCCGCCGACGAAGGGCTCGTCGTGTCGT
TCGACACGGGCCCCGAATGGGCGAACTGGAAGGCGCTGTTCGCGGCGTTTCGCAAGCGCTATCCGAAGGTGGAGCTCACG
TACAACGACATCGGCTCGGCCGCGACGGTCGTCGCGCTCGACAAGTCACGCCGCCGTCCGCAGGCGGACACCGCGTACTA
CTTCGCGGCATCGGCGCTCGACGCGGCTGGCAAGGACGTCGTCGCGCCGTTCAAGCCGGTCAACTTCGACAAGCTCCCGC
CCGTGTTTCGCGAAGCCGACGGCCGTTGGTTCACCGTGCATTCGCTGAATGTCGCGTTCCTCGTCAACCGCAAGCTCGTG
AAGAACGTGCCGCGCCGCTGGTCCGATCTGTTGAAGCCCGAGTACAGGAACGCGGTCGTCTATCTCGACCCGCGTTCGAC
CGGCCAGGGGCAGGTGGTCGTGTTCGCGGCGGCGTCTGCGCTCGGCGGCGGCGTCGACGATCCGAAGCCCGGCGCGGAAT
TCTTCGGAAAGCTGAAGCATGCGGGCAACGTGCTGCGCATCGAGGGCACGACGCCGTATGCGAAGTTCGTCAAGGGTGAG
ATCCCGATCCTGATCGGCTACGAGAACGACGGCCTGAAGGCGAAGTACGCGGACGGCCTGGGCGATGCGGTCGACGTCGT
GATTCCGCAGGACGGCAGCGTGTGCGCGCCGTATGCGATGAGCCTCGTGAAGAACGGGCCGAATCCTGCTGCCGCGCAGC
TATGGTTGAACTTCGTGATGAGCGACGCCGGCCAGGCGCTGTTCGCGCACGGCTACGTGCGGCCCGCAGTGCCGGGCGTC
GCGCTCGCGCCCGACGTCGCGGCGAAGATGCCGAACGCGCCACAGGTGCGTGCGCTCGACGTCGCGAAGGCCGCCGCGCG
CAAGGCCGAAGTCGACCGGCTATGGTCGCAGGCGGCGCTTGGCCAGTAA

Upstream 100 bases:

>100_bases
TGCTCGCCGCGCGCGGCGTCGCGATCGAGCCGCGCCATCTGCTCGTGCTGCCCGCTTCGGGCGGCGCGCGATGATTGTTT
CGTTTAACGGGAGATCCACG

Downstream 100 bases:

>100_bases
GGAGCGCGACGTGCAACGATCGTTCCTCGAACGCAGCCTGTTGCGCCGCTTCGGCGCGGCGCCGGCGGGCGGGCTGCTCG
CGCTTTTCTTCGTGCTGCCG

Product: ABC transporter periplasmic substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSENRPLLTALRRAALAFGMCATLVANGASAEPLYAGEDALYAKAADEGLVVSFDTGPEWANWKALFAAFRKRYPKVELT
YNDIGSAATVVALDKSRRRPQADTAYYFAASALDAAGKDVVAPFKPVNFDKLPPVFREADGRWFTVHSLNVAFLVNRKLV
KNVPRRWSDLLKPEYRNAVVYLDPRSTGQGQVVVFAAASALGGGVDDPKPGAEFFGKLKHAGNVLRIEGTTPYAKFVKGE
IPILIGYENDGLKAKYADGLGDAVDVVIPQDGSVCAPYAMSLVKNGPNPAAAQLWLNFVMSDAGQALFAHGYVRPAVPGV
ALAPDVAAKMPNAPQVRALDVAKAAARKAEVDRLWSQAALGQ

Sequences:

>Translated_362_residues
MSENRPLLTALRRAALAFGMCATLVANGASAEPLYAGEDALYAKAADEGLVVSFDTGPEWANWKALFAAFRKRYPKVELT
YNDIGSAATVVALDKSRRRPQADTAYYFAASALDAAGKDVVAPFKPVNFDKLPPVFREADGRWFTVHSLNVAFLVNRKLV
KNVPRRWSDLLKPEYRNAVVYLDPRSTGQGQVVVFAAASALGGGVDDPKPGAEFFGKLKHAGNVLRIEGTTPYAKFVKGE
IPILIGYENDGLKAKYADGLGDAVDVVIPQDGSVCAPYAMSLVKNGPNPAAAQLWLNFVMSDAGQALFAHGYVRPAVPGV
ALAPDVAAKMPNAPQVRALDVAKAAARKAEVDRLWSQAALGQ
>Mature_361_residues
SENRPLLTALRRAALAFGMCATLVANGASAEPLYAGEDALYAKAADEGLVVSFDTGPEWANWKALFAAFRKRYPKVELTY
NDIGSAATVVALDKSRRRPQADTAYYFAASALDAAGKDVVAPFKPVNFDKLPPVFREADGRWFTVHSLNVAFLVNRKLVK
NVPRRWSDLLKPEYRNAVVYLDPRSTGQGQVVVFAAASALGGGVDDPKPGAEFFGKLKHAGNVLRIEGTTPYAKFVKGEI
PILIGYENDGLKAKYADGLGDAVDVVIPQDGSVCAPYAMSLVKNGPNPAAAQLWLNFVMSDAGQALFAHGYVRPAVPGVA
LAPDVAAKMPNAPQVRALDVAKAAARKAEVDRLWSQAALGQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38735; Mature: 38603

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENRPLLTALRRAALAFGMCATLVANGASAEPLYAGEDALYAKAADEGLVVSFDTGPEW
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
ANWKALFAAFRKRYPKVELTYNDIGSAATVVALDKSRRRPQADTAYYFAASALDAAGKDV
CHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHEEHHHHHCCCCCCC
VAPFKPVNFDKLPPVFREADGRWFTVHSLNVAFLVNRKLVKNVPRRWSDLLKPEYRNAVV
CCCCCCCCCCCCCCHHHCCCCCEEEEEECEEEEEECCHHHHHHHHHHHHHCCCCCCCEEE
YLDPRSTGQGQVVVFAAASALGGGVDDPKPGAEFFGKLKHAGNVLRIEGTTPYAKFVKGE
EECCCCCCCCCEEEEEECHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCC
IPILIGYENDGLKAKYADGLGDAVDVVIPQDGSVCAPYAMSLVKNGPNPAAAQLWLNFVM
CCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SDAGQALFAHGYVRPAVPGVALAPDVAAKMPNAPQVRALDVAKAAARKAEVDRLWSQAAL
HHCCCEEHHCCCCCCCCCCCEECCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
GQ
CC
>Mature Secondary Structure 
SENRPLLTALRRAALAFGMCATLVANGASAEPLYAGEDALYAKAADEGLVVSFDTGPEW
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCC
ANWKALFAAFRKRYPKVELTYNDIGSAATVVALDKSRRRPQADTAYYFAASALDAAGKDV
CHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHEEHHHHHCCCCCCC
VAPFKPVNFDKLPPVFREADGRWFTVHSLNVAFLVNRKLVKNVPRRWSDLLKPEYRNAVV
CCCCCCCCCCCCCCHHHCCCCCEEEEEECEEEEEECCHHHHHHHHHHHHHCCCCCCCEEE
YLDPRSTGQGQVVVFAAASALGGGVDDPKPGAEFFGKLKHAGNVLRIEGTTPYAKFVKGE
EECCCCCCCCCEEEEEECHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHCCC
IPILIGYENDGLKAKYADGLGDAVDVVIPQDGSVCAPYAMSLVKNGPNPAAAQLWLNFVM
CCEEEEECCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SDAGQALFAHGYVRPAVPGVALAPDVAAKMPNAPQVRALDVAKAAARKAEVDRLWSQAAL
HHCCCEEHHCCCCCCCCCCCEECCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
GQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]