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The map label for this gene is pcaB [H]

Identifier: 76819651

GI number: 76819651

Start: 1883311

End: 1884669

Strand: Direct

Name: pcaB [H]

Synonym: BURPS1710b_A1555

Alternate gene names: 76819651

Gene position: 1883311-1884669 (Clockwise)

Preceding gene: 76817759

Following gene: 76819655

Centisome position: 59.19

GC content: 72.04

Gene sequence:

>1359_bases
ATGCTCGAAGACAGTGCCCGTCTGACCGCTCTCGTTTGCGGAAGCGAACCGCTCAACCGGATCTGGTCGCCGCATGCGAC
GTTGCAGCGGATGCTCGACGTCGAAGCGGCGCTCGCGCGCGCGCTCGCCGCGAATGGCGTGATTCCGGCGAACGCGGTGC
GGCCGATCGAGGCGGCCTGCGCGGCGAGCGAGCTCGACACGCAGGCGCTGATGCGCGACGCGGCGCTCGGCGGCAATATC
GCGATTCCGCTCGTCAGGCAATTGACCGCACGCGTGAAGGCGCGCGACGCCGAAGCGGCGAAGTACGTGCACTGGGGCGC
GACGAGCCAGGACATCATCGATACCGCCGCCGTGCTGCAACTGCGCGACACGCTCGACTGGCTCGATCCGCTGCTGCGCG
ATACGTGCACGGCGCTCGCGACGCTCGCGGCCGCGCACCGCACGACACCGATGATCGGTCGCACGTGGCTGCAGCAGGCG
CTGCCGATCACGCTCGGATTGAAGTTCGCGCAATGGCTCGACGCGCTGCTGCGCCACCGCGAGCGTATCGTCGCGTTGCG
CGAGCGCGCGCTCGCATTGCAGTTCGGCGGCGCGGCGGGCACGCTCGCGAGCTTGCGCGACGCGGCGCCCGCGGTTGCGC
GCTCGCTCGCCGACGACCTTCGGCTCGCGCTGCCGGCCGTGCCGTGGCACACGCAGCGCGACCGGATCGCCGAGACGGCG
GCTTGCTTCGGGATGCTGATCGGCACGCTCGGCAAGATCGCGCGCGACATTTCGCTGTCGATGCAAACGGAGATCGGCGA
GCTCGCGGAGCCCGCCGCGGCGGGCAAGGGCGGCTCGTCGACGATGCCGCACAAGCGCAATCCGGTCGGCTGCGCGGCCG
TGCTGACCGCGGCCGTGCGCGCGCCCGGGCTCGTGTCGACCGTGCTCGCCGGCATGGTCCAGGAACACGAGCGCGCGCTC
GGCGGCTGGCAGGCCGAGTGGGACGCGCTGCCCGAACTCGCGCGGCTCGCGGGCGGCGCGCTCGCGCAGGTCGCGCAGAT
CGTCGAGGGATTGAACGTCGACGTCGCGCGCCTTGCCGACAATCTCGACGCGACGCGCGGCCTCGTGCTCGGCGAGGCGG
TGATGCTCGCGCTCGGCGACAGGATCGGCCGGCTCGACGCGCATCATCTCGTCGAGCGCGCCTCGAAGCAAGCCGTGCGC
AGCGGCCGCCCGCTGTACGACGTGCTGGCCGCGGACCCCGACGTCGCCGCGCACCTCACGCCGCAGGCGCTGCGGCAGTT
GCTCGATCCCGCTCATTACGTCGGCGAGGCGCACGCCTATGTCGACGCCGTGCTCGCGCTCCACGCGAGCCGCCATTAA

Upstream 100 bases:

>100_bases
GGCCGTGCGGCGCCCCGCCGCGATTGGCCGAACGCACGAGCGCGCAACGTCCGGCGCTTCGGTGGACAATAAGCGCAATT
CGTCTTTTTCGATCGACCCC

Downstream 100 bases:

>100_bases
GGAGAGATACCCATGCCTTTCGCTTCAGTCGATGGAGTGCGACTGCATTACCGGATCGATCGCGCGGCGCGCGCCGACGC
GCCGTGGCTCGTGTTCTCGA

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLEDSARLTALVCGSEPLNRIWSPHATLQRMLDVEAALARALAANGVIPANAVRPIEAACAASELDTQALMRDAALGGNI
AIPLVRQLTARVKARDAEAAKYVHWGATSQDIIDTAAVLQLRDTLDWLDPLLRDTCTALATLAAAHRTTPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRERIVALRERALALQFGGAAGTLASLRDAAPAVARSLADDLRLALPAVPWHTQRDRIAETA
ACFGMLIGTLGKIARDISLSMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVSTVLAGMVQEHERAL
GGWQAEWDALPELARLAGGALAQVAQIVEGLNVDVARLADNLDATRGLVLGEAVMLALGDRIGRLDAHHLVERASKQAVR
SGRPLYDVLAADPDVAAHLTPQALRQLLDPAHYVGEAHAYVDAVLALHASRH

Sequences:

>Translated_452_residues
MLEDSARLTALVCGSEPLNRIWSPHATLQRMLDVEAALARALAANGVIPANAVRPIEAACAASELDTQALMRDAALGGNI
AIPLVRQLTARVKARDAEAAKYVHWGATSQDIIDTAAVLQLRDTLDWLDPLLRDTCTALATLAAAHRTTPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRERIVALRERALALQFGGAAGTLASLRDAAPAVARSLADDLRLALPAVPWHTQRDRIAETA
ACFGMLIGTLGKIARDISLSMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVSTVLAGMVQEHERAL
GGWQAEWDALPELARLAGGALAQVAQIVEGLNVDVARLADNLDATRGLVLGEAVMLALGDRIGRLDAHHLVERASKQAVR
SGRPLYDVLAADPDVAAHLTPQALRQLLDPAHYVGEAHAYVDAVLALHASRH
>Mature_452_residues
MLEDSARLTALVCGSEPLNRIWSPHATLQRMLDVEAALARALAANGVIPANAVRPIEAACAASELDTQALMRDAALGGNI
AIPLVRQLTARVKARDAEAAKYVHWGATSQDIIDTAAVLQLRDTLDWLDPLLRDTCTALATLAAAHRTTPMIGRTWLQQA
LPITLGLKFAQWLDALLRHRERIVALRERALALQFGGAAGTLASLRDAAPAVARSLADDLRLALPAVPWHTQRDRIAETA
ACFGMLIGTLGKIARDISLSMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVRAPGLVSTVLAGMVQEHERAL
GGWQAEWDALPELARLAGGALAQVAQIVEGLNVDVARLADNLDATRGLVLGEAVMLALGDRIGRLDAHHLVERASKQAVR
SGRPLYDVLAADPDVAAHLTPQALRQLLDPAHYVGEAHAYVDAVLALHASRH

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=369, Percent_Identity=26.5582655826558, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787376, Length=288, Percent_Identity=25.3472222222222, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787896, Length=338, Percent_Identity=27.2189349112426, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=317, Percent_Identity=25.8675078864353, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32564234, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=368, Percent_Identity=26.6304347826087, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI78710009, Length=309, Percent_Identity=27.1844660194175, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640179, Length=350, Percent_Identity=25.1428571428571, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640177, Length=350, Percent_Identity=25.1428571428571, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24662684, Length=347, Percent_Identity=26.2247838616715, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47941; Mature: 47941

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDSARLTALVCGSEPLNRIWSPHATLQRMLDVEAALARALAANGVIPANAVRPIEAAC
CCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AASELDTQALMRDAALGGNIAIPLVRQLTARVKARDAEAAKYVHWGATSQDIIDTAAVLQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHH
LRDTLDWLDPLLRDTCTALATLAAAHRTTPMIGRTWLQQALPITLGLKFAQWLDALLRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ERIVALRERALALQFGGAAGTLASLRDAAPAVARSLADDLRLALPAVPWHTQRDRIAETA
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ACFGMLIGTLGKIARDISLSMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLVSTVLAGMVQEHERALGGWQAEWDALPELARLAGGALAQVAQIVEGLNVDVARLAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHH
NLDATRGLVLGEAVMLALGDRIGRLDAHHLVERASKQAVRSGRPLYDVLAADPDVAAHLT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCC
PQALRQLLDPAHYVGEAHAYVDAVLALHASRH
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEDSARLTALVCGSEPLNRIWSPHATLQRMLDVEAALARALAANGVIPANAVRPIEAAC
CCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
AASELDTQALMRDAALGGNIAIPLVRQLTARVKARDAEAAKYVHWGATSQDIIDTAAVLQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHH
LRDTLDWLDPLLRDTCTALATLAAAHRTTPMIGRTWLQQALPITLGLKFAQWLDALLRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ERIVALRERALALQFGGAAGTLASLRDAAPAVARSLADDLRLALPAVPWHTQRDRIAETA
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ACFGMLIGTLGKIARDISLSMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLTAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLVSTVLAGMVQEHERALGGWQAEWDALPELARLAGGALAQVAQIVEGLNVDVARLAD
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHH
NLDATRGLVLGEAVMLALGDRIGRLDAHHLVERASKQAVRSGRPLYDVLAADPDVAAHLT
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHCCC
PQALRQLLDPAHYVGEAHAYVDAVLALHASRH
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]