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The map label for this gene is vacJ [H]

Identifier: 76819566

GI number: 76819566

Start: 1812576

End: 1813550

Strand: Direct

Name: vacJ [H]

Synonym: BURPS1710b_A1481

Alternate gene names: 76819566

Gene position: 1812576-1813550 (Clockwise)

Preceding gene: 76817859

Following gene: 76819457

Centisome position: 56.97

GC content: 69.74

Gene sequence:

>975_bases
ATGCGAACCACCGCGTCGGCGCTCGCCGTCTGCCTGCTTGCGAGCGGCTGCGCGACCGGCCCCAACCGGCACCCGGGCGA
TCCGCTCGAGCCGATGAACCGCGCCGTTTTCCAGTTCAACGACGCGGTCGACTCCGCGATCGCCGTGCCGATCGCGAAGG
GCTATCAAAAAGTAACGCCCACGCCGCTGCGCACGGCGATCAGCAATTTCTTCTCGAATCTCGGCGATCTCGGCAACATC
GCGAACAACCTGCTGCAGCTCCGGATCACCGATGCGACACAGGACGTGATGCGCGTCGCGATGAACTCGGTGTTCGGCGT
CGCCGGGCTGATCGATGTCGCGTCTCTCGCCGGGCTGCCGAAGCACCACCAGGACTTCGGCCTCACGCTCGCGCGCTGGG
GCGTGCCGGCCGGCCCGTATCTGGTGCTGCCGGTCTTCGGGCCGAGCTCGATTCGCGACGGTGTCGGCCGCGCGGTCGAC
GTCCGCTTCAATCTGCTGAATTACATCGAGCCTGCCGCGCGCAATCCGATGTACATCGCGCAGTTCATCAGCGCGCGCTC
GGATCTGCTCGGGGCGACCGATCTGCTGCAGCAGGCGGCGCTCGACAAGTACTCGTTCGTGCGCGACGCATACGTGCAGC
AGCGCCGCTCGCTCACGTATCACGGCAGAAGCGACGACGAATCGCTGCCGCACTACGACGAGCCCGGCGGCGAGCCGGCG
GGCGGCCCGGGCGGCGGCGCGCCGGGCGGCTTGCCGCAATACGAGGATCCGGGCGAGGGCGCGGGCGGCATGCCGCCGGG
CGGCGCGCGCGAGCCCGCCGGCCTGCCGCAGTATCAGGACCCGGGCGACGAAGCGGCGGCGTCGGCAGCGGCATCCGCCG
CTTCATCCGCTTCATCCGCTTCATCCGCGTCGCCGGCGTCGTCAGTCGCGCCGGCTTCCGCGTCGTCCGCACAGTCTCCG
TCGTCGCCGAACTGA

Upstream 100 bases:

>100_bases
TCGTGCACGCTGATCGGCGCCGTCGTGTTCCAGCCCGTGCTGATGGGCAAGCCGCGCACGAAGCGCGTGACGAATCAATC
GCAAGGAATCGATGAATAAG

Downstream 100 bases:

>100_bases
CTGCTGCCCCACTCATCGACACGGCGGCCGTTTCGCATCCGAAACGGCCGCCGTCGCGTTTTCCGGGCAGCGCGCGCGTC
GCGCGACGCCGGCCGTGCTC

Product: lipoprotein VacJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRTTASALAVCLLASGCATGPNRHPGDPLEPMNRAVFQFNDAVDSAIAVPIAKGYQKVTPTPLRTAISNFFSNLGDLGNI
ANNLLQLRITDATQDVMRVAMNSVFGVAGLIDVASLAGLPKHHQDFGLTLARWGVPAGPYLVLPVFGPSSIRDGVGRAVD
VRFNLLNYIEPAARNPMYIAQFISARSDLLGATDLLQQAALDKYSFVRDAYVQQRRSLTYHGRSDDESLPHYDEPGGEPA
GGPGGGAPGGLPQYEDPGEGAGGMPPGGAREPAGLPQYQDPGDEAAASAAASAASSASSASSASPASSVAPASASSAQSP
SSPN

Sequences:

>Translated_324_residues
MRTTASALAVCLLASGCATGPNRHPGDPLEPMNRAVFQFNDAVDSAIAVPIAKGYQKVTPTPLRTAISNFFSNLGDLGNI
ANNLLQLRITDATQDVMRVAMNSVFGVAGLIDVASLAGLPKHHQDFGLTLARWGVPAGPYLVLPVFGPSSIRDGVGRAVD
VRFNLLNYIEPAARNPMYIAQFISARSDLLGATDLLQQAALDKYSFVRDAYVQQRRSLTYHGRSDDESLPHYDEPGGEPA
GGPGGGAPGGLPQYEDPGEGAGGMPPGGAREPAGLPQYQDPGDEAAASAAASAASSASSASSASPASSVAPASASSAQSP
SSPN
>Mature_324_residues
MRTTASALAVCLLASGCATGPNRHPGDPLEPMNRAVFQFNDAVDSAIAVPIAKGYQKVTPTPLRTAISNFFSNLGDLGNI
ANNLLQLRITDATQDVMRVAMNSVFGVAGLIDVASLAGLPKHHQDFGLTLARWGVPAGPYLVLPVFGPSSIRDGVGRAVD
VRFNLLNYIEPAARNPMYIAQFISARSDLLGATDLLQQAALDKYSFVRDAYVQQRRSLTYHGRSDDESLPHYDEPGGEPA
GGPGGGAPGGLPQYEDPGEGAGGMPPGGAREPAGLPQYQDPGDEAAASAAASAASSASSASSASPASSVAPASASSAQSP
SSPN

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=222, Percent_Identity=33.7837837837838, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 33314; Mature: 33314

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTASALAVCLLASGCATGPNRHPGDPLEPMNRAVFQFNDAVDSAIAVPIAKGYQKVTP
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
TPLRTAISNFFSNLGDLGNIANNLLQLRITDATQDVMRVAMNSVFGVAGLIDVASLAGLP
CHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KHHQDFGLTLARWGVPAGPYLVLPVFGPSSIRDGVGRAVDVRFNLLNYIEPAARNPMYIA
CCHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHCCHHHHH
QFISARSDLLGATDLLQQAALDKYSFVRDAYVQQRRSLTYHGRSDDESLPHYDEPGGEPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GGPGGGAPGGLPQYEDPGEGAGGMPPGGAREPAGLPQYQDPGDEAAASAAASAASSASSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SSASPASSVAPASASSAQSPSSPN
CCCCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTTASALAVCLLASGCATGPNRHPGDPLEPMNRAVFQFNDAVDSAIAVPIAKGYQKVTP
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
TPLRTAISNFFSNLGDLGNIANNLLQLRITDATQDVMRVAMNSVFGVAGLIDVASLAGLP
CHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KHHQDFGLTLARWGVPAGPYLVLPVFGPSSIRDGVGRAVDVRFNLLNYIEPAARNPMYIA
CCHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHCCHHHHH
QFISARSDLLGATDLLQQAALDKYSFVRDAYVQQRRSLTYHGRSDDESLPHYDEPGGEPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GGPGGGAPGGLPQYEDPGEGAGGMPPGGAREPAGLPQYQDPGDEAAASAAASAASSASSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SSASPASSVAPASASSAQSPSSPN
CCCCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]