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The map label for this gene is paaF [C]

Identifier: 76819358

GI number: 76819358

Start: 1435749

End: 1436591

Strand: Direct

Name: paaF [C]

Synonym: BURPS1710b_A1151

Alternate gene names: 76819358

Gene position: 1435749-1436591 (Clockwise)

Preceding gene: 76817519

Following gene: 76817578

Centisome position: 45.12

GC content: 77.11

Gene sequence:

>843_bases
ATGACCGGGACGACTTTCTCGACGCCCGCGCCGCTGCATGTCGCGCAAGCCGACGGCGTGCGGTTCGCGACGCTGAACCG
GCCGGCCAGGCGCAATGCGCTGAGCGACGATCTCGTCGCCGCGCTCGACGCCGAATGCGAGCGCGCCGCGGGCGACGCCG
CCGTGCGCGCGCTCGTGCTGCGCGGCGCGGGCGGCATGTTCTGCGCGGGCGGCGATTTCGGCGGCTTCAGCGCGATGATG
CGCGAGCCGGCGCCCGCGGGCGAACCCGATCCGATCGCGGCGGCGAACCGGCGGTTCGGCGCGCTGCTCGAAAAGCTCGC
GGCGTTGCCGGTGCCGACGCTCGCCGTCGTCGAGGGCGCGGCGGCGGGCGGCGGCTGCGGGCTCGCGGCCGCGTGCGATC
GCGTGCTGATCGCGAGCGACGCGCAGCTTTCGTTACCCGAAACGTCGCTCGGGCTGCCGCCCGCGCAGATCGCGCCGTTC
ATCGTCGCGCGCGCGGGCGCCGCGCGTGGCTGCTGGCTGATGCTGACGGCCGGCCGGCTGTCGGCGGCCGACGCGCTGGA
TGCGGGCCTCGCCGACGAAATCGCGGGGGCCGACGGCATCGACGCGCTGCTGCGCCGCGCGCTCGCGCGCGTGCTCGCAT
GCGAGCCGGCCGCGCTGCGCGCGACGAAGCGCATCGTCGCCGATGCGCTGCGCCGCGAGCGCGGCGCGGCCCTCGATGCG
GCCTCCGGCGAGTTTGCGGCTGCACTGCGCTCGGGGGCCGTCGCCGAAGGGCTCGCCGCGTTGTCCGGCAAGCGCGCGCC
GCGCTGGGCGAGCGACGCCCCCTTGCCGCCGGAGGCGCGATGA

Upstream 100 bases:

>100_bases
CCGCGACACGCGGCTCTTCTCGATCGGCGGCGGCGCGGACGAAGTGATGCTCGGCATCATCTGCAAGAAGCTCGGCACGC
TGCCGCGGGAGTGACGCGCG

Downstream 100 bases:

>100_bases
GCACGCGCCGCTTCACCCGGCTTCTGATCGCCAACCGCGGCGAGATCGCGGTGCGCATCGCGCGCACCGCGCGGCGGCTC
GGCATCGCGACGATCGCCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTGTTFSTPAPLHVAQADGVRFATLNRPARRNALSDDLVAALDAECERAAGDAAVRALVLRGAGGMFCAGGDFGGFSAMM
REPAPAGEPDPIAAANRRFGALLEKLAALPVPTLAVVEGAAAGGGCGLAAACDRVLIASDAQLSLPETSLGLPPAQIAPF
IVARAGAARGCWLMLTAGRLSAADALDAGLADEIAGADGIDALLRRALARVLACEPAALRATKRIVADALRRERGAALDA
ASGEFAAALRSGAVAEGLAALSGKRAPRWASDAPLPPEAR

Sequences:

>Translated_280_residues
MTGTTFSTPAPLHVAQADGVRFATLNRPARRNALSDDLVAALDAECERAAGDAAVRALVLRGAGGMFCAGGDFGGFSAMM
REPAPAGEPDPIAAANRRFGALLEKLAALPVPTLAVVEGAAAGGGCGLAAACDRVLIASDAQLSLPETSLGLPPAQIAPF
IVARAGAARGCWLMLTAGRLSAADALDAGLADEIAGADGIDALLRRALARVLACEPAALRATKRIVADALRRERGAALDA
ASGEFAAALRSGAVAEGLAALSGKRAPRWASDAPLPPEAR
>Mature_279_residues
TGTTFSTPAPLHVAQADGVRFATLNRPARRNALSDDLVAALDAECERAAGDAAVRALVLRGAGGMFCAGGDFGGFSAMMR
EPAPAGEPDPIAAANRRFGALLEKLAALPVPTLAVVEGAAAGGGCGLAAACDRVLIASDAQLSLPETSLGLPPAQIAPFI
VARAGAARGCWLMLTAGRLSAADALDAGLADEIAGADGIDALLRRALARVLACEPAALRATKRIVADALRRERGAALDAA
SGEFAAALRSGAVAEGLAALSGKRAPRWASDAPLPPEAR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=277, Percent_Identity=27.7978339350181, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI31542718, Length=275, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=30.5084745762712, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=29.4776119402985, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=28.1914893617021, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=229, Percent_Identity=24.4541484716157, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=28.1914893617021, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=229, Percent_Identity=24.4541484716157, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI70995211, Length=199, Percent_Identity=29.1457286432161, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=30.188679245283, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=34.3511450381679, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=29.5918367346939, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=262, Percent_Identity=28.6259541984733, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=28.6458333333333, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=280, Percent_Identity=26.4285714285714, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=24.6445497630332, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=30.3703703703704, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=24.4274809160305, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI20129971, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24653477, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28571730, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571729, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28103; Mature: 27972

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTTFSTPAPLHVAQADGVRFATLNRPARRNALSDDLVAALDAECERAAGDAAVRALVL
CCCCCCCCCCCEEEECCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RGAGGMFCAGGDFGGFSAMMREPAPAGEPDPIAAANRRFGALLEKLAALPVPTLAVVEGA
HCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCC
AAGGGCGLAAACDRVLIASDAQLSLPETSLGLPPAQIAPFIVARAGAARGCWLMLTAGRL
CCCCCCCHHHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCC
SAADALDAGLADEIAGADGIDALLRRALARVLACEPAALRATKRIVADALRRERGAALDA
CHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
ASGEFAAALRSGAVAEGLAALSGKRAPRWASDAPLPPEAR
CCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGTTFSTPAPLHVAQADGVRFATLNRPARRNALSDDLVAALDAECERAAGDAAVRALVL
CCCCCCCCCCEEEECCCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RGAGGMFCAGGDFGGFSAMMREPAPAGEPDPIAAANRRFGALLEKLAALPVPTLAVVEGA
HCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCC
AAGGGCGLAAACDRVLIASDAQLSLPETSLGLPPAQIAPFIVARAGAARGCWLMLTAGRL
CCCCCCCHHHHHCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCC
SAADALDAGLADEIAGADGIDALLRRALARVLACEPAALRATKRIVADALRRERGAALDA
CHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
ASGEFAAALRSGAVAEGLAALSGKRAPRWASDAPLPPEAR
CCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA