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The map label for this gene is wtpB [H]

Identifier: 76819269

GI number: 76819269

Start: 2173427

End: 2174275

Strand: Direct

Name: wtpB [H]

Synonym: BURPS1710b_A1784

Alternate gene names: 76819269

Gene position: 2173427-2174275 (Clockwise)

Preceding gene: 76817565

Following gene: 76819274

Centisome position: 68.31

GC content: 67.49

Gene sequence:

>849_bases
ATGCGTTCATCCACCACCGCCGGGTTTGCCGGCGGTATTCCGTTTCGTCGCGGCAAGCGGCGCCCGTCGTTCGGCGCCAT
CGTCGTCAACGGCGTCGTCGCGCTGTTCGCGCTGTATCTACTGATGCCGATCGTGCTGCTGCTGATCGGATCGATCGGGC
AGAGTTGGACGAACACGCTGCTGCCCGCCGGCTTCACGTGGCGCTGGTTCGCCGAACTGGCCGCCGATCCGAGCTTGCGC
CGCGCGTTCGGCGTGAGCTTCGTCGTCGCGCTCGCGTGCTGCGCGCTCGACACGTTGGCCGGCGTGCCGCTCGCGTATGC
GCTGCATCATCGCGCGCTTCGCGGCCGCGGCGTTGCCGCACGCGTCGTGATGCTGACGCCGATCGGCGTGCCGGCGCTCA
CGCTCGGCTTCGGCTACATCGTGGTATTCGGCGGCGACGCGTTGCCGTGGCTCGGCACGCTGCCGCTGATGATCGCCGCG
CATGCGGTGCTGACGCTGCCGTATCTGCTGCAGACGTTGTTGAGCGATCTGCGCCACCTCGATCTCGCGAAGCTCGAGGC
CTGTGCGGCAACGCTCGGCGCGCCGCCGCTGCGGCAGTTTTTCACGATCGTGCTGCCGAACCTGCGGCAGAGCCTTTTTT
CCGGGCTCGTGATGGTCGCGGCGCTATCGATCGGCGAGTTCCAGGTATCGAACCTGATCGCCGGCTTTCGCTACCGCAAT
TTCCCCGTCGTGCTGCTGCAGGCGTTCTACGGCGCGTCCGGCATCGCCTGCGCGGCGAGTGTCGTGCTGCTGCTGCTCGC
CGTACTCGCGACGCTCGCGTCGACCCACACCGTGCAACGACTGAAGTGA

Upstream 100 bases:

>100_bases
GTCGCGCGCCCACACTGGCGGCCGTCGTCGCGATTCGCGCGACGACTGCTCCGGCGGGCGTTTCGCGTGATGCTTCCCTT
CGTCGCCCGTGAGTGCCGCC

Downstream 100 bases:

>100_bases
AGCCATGAGTCTCGAATTCGACAACGTCAGTTTCAGATATCCGGGCGGGGCGCACGGCGTCGACGCCGTCACGCTGCACG
CGCAGCCGGGCGAGCTGCTC

Product: putative ABC transporter membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRSSTTAGFAGGIPFRRGKRRPSFGAIVVNGVVALFALYLLMPIVLLLIGSIGQSWTNTLLPAGFTWRWFAELAADPSLR
RAFGVSFVVALACCALDTLAGVPLAYALHHRALRGRGVAARVVMLTPIGVPALTLGFGYIVVFGGDALPWLGTLPLMIAA
HAVLTLPYLLQTLLSDLRHLDLAKLEACAATLGAPPLRQFFTIVLPNLRQSLFSGLVMVAALSIGEFQVSNLIAGFRYRN
FPVVLLQAFYGASGIACAASVVLLLLAVLATLASTHTVQRLK

Sequences:

>Translated_282_residues
MRSSTTAGFAGGIPFRRGKRRPSFGAIVVNGVVALFALYLLMPIVLLLIGSIGQSWTNTLLPAGFTWRWFAELAADPSLR
RAFGVSFVVALACCALDTLAGVPLAYALHHRALRGRGVAARVVMLTPIGVPALTLGFGYIVVFGGDALPWLGTLPLMIAA
HAVLTLPYLLQTLLSDLRHLDLAKLEACAATLGAPPLRQFFTIVLPNLRQSLFSGLVMVAALSIGEFQVSNLIAGFRYRN
FPVVLLQAFYGASGIACAASVVLLLLAVLATLASTHTVQRLK
>Mature_282_residues
MRSSTTAGFAGGIPFRRGKRRPSFGAIVVNGVVALFALYLLMPIVLLLIGSIGQSWTNTLLPAGFTWRWFAELAADPSLR
RAFGVSFVVALACCALDTLAGVPLAYALHHRALRGRGVAARVVMLTPIGVPALTLGFGYIVVFGGDALPWLGTLPLMIAA
HAVLTLPYLLQTLLSDLRHLDLAKLEACAATLGAPPLRQFFTIVLPNLRQSLFSGLVMVAALSIGEFQVSNLIAGFRYRN
FPVVLLQAFYGASGIACAASVVLLLLAVLATLASTHTVQRLK

Specific function: Part of the ABC transporter complex wtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=215, Percent_Identity=29.7674418604651, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29981; Mature: 29981

Theoretical pI: Translated: 11.07; Mature: 11.07

Prosite motif: PS00435 PEROXIDASE_1 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSTTAGFAGGIPFRRGKRRPSFGAIVVNGVVALFALYLLMPIVLLLIGSIGQSWTNTL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LPAGFTWRWFAELAADPSLRRAFGVSFVVALACCALDTLAGVPLAYALHHRALRGRGVAA
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHE
RVVMLTPIGVPALTLGFGYIVVFGGDALPWLGTLPLMIAAHAVLTLPYLLQTLLSDLRHL
EEEEECCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAKLEACAATLGAPPLRQFFTIVLPNLRQSLFSGLVMVAALSIGEFQVSNLIAGFRYRN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FPVVLLQAFYGASGIACAASVVLLLLAVLATLASTHTVQRLK
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSSTTAGFAGGIPFRRGKRRPSFGAIVVNGVVALFALYLLMPIVLLLIGSIGQSWTNTL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LPAGFTWRWFAELAADPSLRRAFGVSFVVALACCALDTLAGVPLAYALHHRALRGRGVAA
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHE
RVVMLTPIGVPALTLGFGYIVVFGGDALPWLGTLPLMIAAHAVLTLPYLLQTLLSDLRHL
EEEEECCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAKLEACAATLGAPPLRQFFTIVLPNLRQSLFSGLVMVAALSIGEFQVSNLIAGFRYRN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FPVVLLQAFYGASGIACAASVVLLLLAVLATLASTHTVQRLK
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]