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The map label for this gene is phnV [H]

Identifier: 76819192

GI number: 76819192

Start: 2319134

End: 2319988

Strand: Direct

Name: phnV [H]

Synonym: BURPS1710b_A1903

Alternate gene names: 76819192

Gene position: 2319134-2319988 (Clockwise)

Preceding gene: 76818633

Following gene: 76818160

Centisome position: 72.89

GC content: 76.02

Gene sequence:

>855_bases
ATGTCCGCTGATTTCCCGGCTCGCGCCGCGCCGGTGCCGCGCCGCGAGCTGCGCTGGACCGATGCGGCCTCGCGCGTCGC
CTCGCGTGCGCTCGTCGCGCTCGCGGCCGCCGCCTGCTGCTGGCTGTTCGTGCTGCCCGTCGTCGTCGTCGCGCTGTCGA
GCGTCGCCGGGCACTGGTCGGGCACGATCCTGCCGGCGGGCTACAGCCTGCGTTGGTTCGAGCGCCTCGGCCGGCCCGAA
TTCGACGCGCTCGCCGCGAGCCTCGAGATCGGGCTCGGCGTCGCCGCGCTCGGCACCGCGCTCGGCGTCGCGCTGGCGCT
CGCGCTCGAAGGCCGCACGCGGCGCGGCGTCGGTGCGCTCGTCGACGCGCTCGTGATGCTGCCCAACGGCGTGCCGAGCG
TCGTGCTCGGGCTCGCGGTGCTGATCGCGTATCACGCGAAGCCCGTCGACCTGTCGAGCTCGCCCGCGATCGTCGTGTTC
GTGCAGCTCGCGCTCGTGCTGCCGTTCTGCTACCGGTGCGCGGCCGCCGCGCTGCGCCCCGAGCTGACCGTGCTGCGCGA
GGCGGCCGCGAGCCTCGGCGCGCCGCCCGCGATGGTGCTGCGCCGCGTGCTGCTGCCGCAGCTCGTGCCGGCGATCCGCG
CGAGCCTCGCGCTCGGTTTCGCGCTGTCGCTCGGCGAGCTCGGCGCGACGCTGACCGTCTATCCGCCGGGCTTCGCGACC
GTGCCGATCGTCGTGATCGGCGCGGTCGAGCGCGGCTACTACCTGCCCGCGTCGGCGCTGTCGCTGCTGCTGCTCGGCGC
GTCGCTCGTCGCGCTCATCGCGATCGCGGCGCGCGCGCCGCGGCCGGCGCGATGA

Upstream 100 bases:

>100_bases
CCGGCCGCGGCCGCGGGCGCGGTCGTGATGCTCGCGATGTCGCTCACGCTCTATGCGCTGTACCGGCGGATGAGCCGCCG
CGCGAACGAAGGAGGGCGCG

Downstream 100 bases:

>100_bases
CGGAGACGATGACCGGCGCGGCGGCGCGCCGCTCGCGTGTAACATGAACGAAGGGCCCGAAGGGCGAAACACCATGAATC
GACACATCGAAGTCAACGGC

Product: putative ABC transporter membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSADFPARAAPVPRRELRWTDAASRVASRALVALAAAACCWLFVLPVVVVALSSVAGHWSGTILPAGYSLRWFERLGRPE
FDALAASLEIGLGVAALGTALGVALALALEGRTRRGVGALVDALVMLPNGVPSVVLGLAVLIAYHAKPVDLSSSPAIVVF
VQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPAMVLRRVLLPQLVPAIRASLALGFALSLGELGATLTVYPPGFAT
VPIVVIGAVERGYYLPASALSLLLLGASLVALIAIAARAPRPAR

Sequences:

>Translated_284_residues
MSADFPARAAPVPRRELRWTDAASRVASRALVALAAAACCWLFVLPVVVVALSSVAGHWSGTILPAGYSLRWFERLGRPE
FDALAASLEIGLGVAALGTALGVALALALEGRTRRGVGALVDALVMLPNGVPSVVLGLAVLIAYHAKPVDLSSSPAIVVF
VQLALVLPFCYRCAAAALRPELTVLREAAASLGAPPAMVLRRVLLPQLVPAIRASLALGFALSLGELGATLTVYPPGFAT
VPIVVIGAVERGYYLPASALSLLLLGASLVALIAIAARAPRPAR
>Mature_283_residues
SADFPARAAPVPRRELRWTDAASRVASRALVALAAAACCWLFVLPVVVVALSSVAGHWSGTILPAGYSLRWFERLGRPEF
DALAASLEIGLGVAALGTALGVALALALEGRTRRGVGALVDALVMLPNGVPSVVLGLAVLIAYHAKPVDLSSSPAIVVFV
QLALVLPFCYRCAAAALRPELTVLREAAASLGAPPAMVLRRVLLPQLVPAIRASLALGFALSLGELGATLTVYPPGFATV
PIVVIGAVERGYYLPASALSLLLLGASLVALIAIAARAPRPAR

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=210, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017661
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29269; Mature: 29138

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADFPARAAPVPRRELRWTDAASRVASRALVALAAAACCWLFVLPVVVVALSSVAGHWS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTILPAGYSLRWFERLGRPEFDALAASLEIGLGVAALGTALGVALALALEGRTRRGVGAL
CCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VDALVMLPNGVPSVVLGLAVLIAYHAKPVDLSSSPAIVVFVQLALVLPFCYRCAAAALRP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH
ELTVLREAAASLGAPPAMVLRRVLLPQLVPAIRASLALGFALSLGELGATLTVYPPGFAT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
VPIVVIGAVERGYYLPASALSLLLLGASLVALIAIAARAPRPAR
HHHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SADFPARAAPVPRRELRWTDAASRVASRALVALAAAACCWLFVLPVVVVALSSVAGHWS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTILPAGYSLRWFERLGRPEFDALAASLEIGLGVAALGTALGVALALALEGRTRRGVGAL
CCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VDALVMLPNGVPSVVLGLAVLIAYHAKPVDLSSSPAIVVFVQLALVLPFCYRCAAAALRP
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH
ELTVLREAAASLGAPPAMVLRRVLLPQLVPAIRASLALGFALSLGELGATLTVYPPGFAT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
VPIVVIGAVERGYYLPASALSLLLLGASLVALIAIAARAPRPAR
HHHHEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA