Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 76819159

GI number: 76819159

Start: 701603

End: 702457

Strand: Direct

Name: nadE [H]

Synonym: BURPS1710b_A0515

Alternate gene names: 76819159

Gene position: 701603-702457 (Clockwise)

Preceding gene: 76817493

Following gene: 76819317

Centisome position: 22.05

GC content: 70.29

Gene sequence:

>855_bases
ATGTCCCGTCCCGATCAGGCCGCCCGCCGGCGTGCGATCGCCGCCGAACTGCACGTGTCGCCCACGTTCGATGCGCGCGA
CGAAGCCGAGCGCCGCATCGGGTTCGTCGCCGATTATCTGCGCACGGCCGGATTGCGGGCCTGCGTGCTGGGCATCAGCG
GCGGCATCGATTCGTCGACGGCGGGCCGGCTCGCGCAACTGGCCGTCGAGCGGCTGCGCGCATCGGGCTACGACGCGCGC
TTCGTCGCGATGCGGCTGCCGTACGGCGCGCAGCACGACGAAGCGGACGCGCGGCGCGCGCTCGCCTTCGTGCGCGCGGA
CGAGACGCTCACGGTCGACGTGAAGCCGGCCGCCGATGCGATGCTCGCCGCGCTCGCGGCGGGCGGCCTGGCGTATCTCG
ACCACGCACAGCAGGATTTCGTGCTCGGCAACATCAAGGCGCGCGAGCGGATGATCGCGCAATACGCGGTGGCGGGCGCG
CGGAACGGCGTCGTGATCGGCACCGATCATGCGGCGGAATCGGTGATGGGCTTCTTCACGAAGTTCGGCGACGGCGGCGC
GGACGTGCTGCCGCTCGCGGGCCTCACGAAACGCCGCGTGCGCGCACTCGCGCGCATGCTGGGCGCCGACGAGCCGCTCG
TGCTGAAAACGCCGACCGCCGATCTCGAAACGTTGCGTCCGCAACGGCCCGACGAGCACGCGTACGGCATCACGTACGAG
CAGATCGACGATTTTCTCGAAGGCAAGCCGATGGACGACGCGGTCGCCGAGACGGTGCTGCGCTTCTATGACGCGACACG
CCACAAGCGCGCGCTGCCCTATACGATGTTCGACTGGCCGGGCCACCCGGCATGA

Upstream 100 bases:

>100_bases
CGACACCCGACGACCGGATCGACAGCCGAATCCGCCGCCGGCAAGCCCGCGCGAATCCGCCTATGATCGGTTCTTCGATT
CCGCCGCGAAGGAGCACGCG

Downstream 100 bases:

>100_bases
GCGAGGAGGAAGACGAAGCCCGCGGCGGCTCGGCGCCGCCGGTTCGGCGGTCGTTTCCGCCGCCGCACCGCTGCGGTCCA
CGATCGCGCACGCGCGCCGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDAR
FVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGA
RNGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
QIDDFLEGKPMDDAVAETVLRFYDATRHKRALPYTMFDWPGHPA

Sequences:

>Translated_284_residues
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDAR
FVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGA
RNGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
QIDDFLEGKPMDDAVAETVLRFYDATRHKRALPYTMFDWPGHPA
>Mature_283_residues
SRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSSTAGRLAQLAVERLRASGYDARF
VAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADAMLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGAR
NGVVIGTDHAAESVMGFFTKFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYEQ
IDDFLEGKPMDDAVAETVLRFYDATRHKRALPYTMFDWPGHPA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=272, Percent_Identity=48.1617647058824, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30882; Mature: 30751

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSST
CCCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHH
AGRLAQLAVERLRASGYDARFVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHH
MLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGARNGVVIGTDHAAESVMGFFT
HHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
HHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHH
QIDDFLEGKPMDDAVAETVLRFYDATRHKRALPYTMFDWPGHPA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SRPDQAARRRAIAAELHVSPTFDARDEAERRIGFVADYLRTAGLRACVLGISGGIDSST
CCCHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHH
AGRLAQLAVERLRASGYDARFVAMRLPYGAQHDEADARRALAFVRADETLTVDVKPAADA
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCEEEEECCCHHHH
MLAALAAGGLAYLDHAQQDFVLGNIKARERMIAQYAVAGARNGVVIGTDHAAESVMGFFT
HHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHH
KFGDGGADVLPLAGLTKRRVRALARMLGADEPLVLKTPTADLETLRPQRPDEHAYGITYE
HHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCCCCCCCCCCCHH
QIDDFLEGKPMDDAVAETVLRFYDATRHKRALPYTMFDWPGHPA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA