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The map label for this gene is hpcB [H]

Identifier: 76819023

GI number: 76819023

Start: 2056502

End: 2057428

Strand: Direct

Name: hpcB [H]

Synonym: BURPS1710b_A1686

Alternate gene names: 76819023

Gene position: 2056502-2057428 (Clockwise)

Preceding gene: 76819510

Following gene: 76817475

Centisome position: 64.63

GC content: 70.98

Gene sequence:

>927_bases
ATGGGAAAGATCGTCGGCGCCGGGCTCATTTCGCACGCGCCCGTCGTGATGATGCCGCAGGCCGTGCGGCTGCGCGAAAA
CGGCGGCCGGGACTTCACGCTCGCAACCGGCCTCGCCCGGTTGCGGCGCGACGTGTTCGACGCGCACGATTACGATACGG
TGCTCGTTTTCGACAGCCATTGGCTCACGACGACCGAAGCGGTGGTCACCGCGCATGCGCGGCGCGCGGGCCGCTTCACG
TCGGACGAGATGCCGAACGCGATCCGGCAACTGCCATACGATCTGGCGGGCGACCCGGCGCTCGCGCATGCGATCGCCGA
GCAGGCGCGGCGGCGTGCGTCGTGGATCGACGCGGTGGACGACCCGTGCCTGCCGCTGCACTACGCGACGCTCAATCCGT
GGACCTATCTCGGCCGCCCGGACAAGCGCTGGCTTTCCGTCTCCGTGTGCCAGACCGCGACGACCGAGGATTTCCTGCGG
ATGGGCGAAACCGTCGCGCAGGCGATCGCGCAGCTCGAGCGCAAGGTGCTGCTCGTCGCGTCGGGCGGCCTGTCGCATGC
GTTCTGGCCGCTCGCCGAGCTGCGCGGGCGGATGGCGGGCGCGGCGTCGAACATCGTCACGCCCGCCGCGCGCGCGGCGG
ACGAGCGGCGGATCGCGTGGCTCGAGCAAGGGCGGCACGACCGGGTGATCGACGCGATGCCGGAATTCCTGCGCTTCGCG
CCGGAGGCGAACTTCGGCCACTACCTGATGATGGCGGGCGCGCTCGGCGCGCGCGCGTGCGCGGCGCGTGCACGCCGCTT
CAGCGAGTACGAGAACGGCATCGGCACCGGGCACGTCCATCTGTGGTTCAGTCCCGCCGACGGCGGATGGGCGCGGGCCG
AAACGCACAACGAACGGCGCACCGAAACGGACGCGGCGAGCGCTTGA

Upstream 100 bases:

>100_bases
TCTCGCCGCGCGCCGCACGACCGGCAAGCTGCTGCTCGTGCCGTGAGCCCCCACCGCTCGCCCGCTCATTCACTCGTTCA
TCGAACAGGAGGCCTAATCC

Downstream 100 bases:

>100_bases
GCGGCGCGATGCGACGTGCAGGCGGGCGACGGCGCTCATGCCGGCGCCCGCGCGGCCAGCTGTTTGTGACGCCGGCCGAT
TGTGCCGCCGTGCGCGCGAC

Product: putative dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MGKIVGAGLISHAPVVMMPQAVRLRENGGRDFTLATGLARLRRDVFDAHDYDTVLVFDSHWLTTTEAVVTAHARRAGRFT
SDEMPNAIRQLPYDLAGDPALAHAIAEQARRRASWIDAVDDPCLPLHYATLNPWTYLGRPDKRWLSVSVCQTATTEDFLR
MGETVAQAIAQLERKVLLVASGGLSHAFWPLAELRGRMAGAASNIVTPAARAADERRIAWLEQGRHDRVIDAMPEFLRFA
PEANFGHYLMMAGALGARACAARARRFSEYENGIGTGHVHLWFSPADGGWARAETHNERRTETDAASA

Sequences:

>Translated_308_residues
MGKIVGAGLISHAPVVMMPQAVRLRENGGRDFTLATGLARLRRDVFDAHDYDTVLVFDSHWLTTTEAVVTAHARRAGRFT
SDEMPNAIRQLPYDLAGDPALAHAIAEQARRRASWIDAVDDPCLPLHYATLNPWTYLGRPDKRWLSVSVCQTATTEDFLR
MGETVAQAIAQLERKVLLVASGGLSHAFWPLAELRGRMAGAASNIVTPAARAADERRIAWLEQGRHDRVIDAMPEFLRFA
PEANFGHYLMMAGALGARACAARARRFSEYENGIGTGHVHLWFSPADGGWARAETHNERRTETDAASA
>Mature_307_residues
GKIVGAGLISHAPVVMMPQAVRLRENGGRDFTLATGLARLRRDVFDAHDYDTVLVFDSHWLTTTEAVVTAHARRAGRFTS
DEMPNAIRQLPYDLAGDPALAHAIAEQARRRASWIDAVDDPCLPLHYATLNPWTYLGRPDKRWLSVSVCQTATTEDFLRM
GETVAQAIAQLERKVLLVASGGLSHAFWPLAELRGRMAGAASNIVTPAARAADERRIAWLEQGRHDRVIDAMPEFLRFAP
EANFGHYLMMAGALGARACAARARRFSEYENGIGTGHVHLWFSPADGGWARAETHNERRTETDAASA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 33936; Mature: 33805

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIVGAGLISHAPVVMMPQAVRLRENGGRDFTLATGLARLRRDVFDAHDYDTVLVFDSH
CCCCCCCHHHHCCCEEECCHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEECC
WLTTTEAVVTAHARRAGRFTSDEMPNAIRQLPYDLAGDPALAHAIAEQARRRASWIDAVD
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DPCLPLHYATLNPWTYLGRPDKRWLSVSVCQTATTEDFLRMGETVAQAIAQLERKVLLVA
CCCCEEEEEECCCCEECCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEE
SGGLSHAFWPLAELRGRMAGAASNIVTPAARAADERRIAWLEQGRHDRVIDAMPEFLRFA
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PEANFGHYLMMAGALGARACAARARRFSEYENGIGTGHVHLWFSPADGGWARAETHNERR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHH
TETDAASA
CCCCCCCC
>Mature Secondary Structure 
GKIVGAGLISHAPVVMMPQAVRLRENGGRDFTLATGLARLRRDVFDAHDYDTVLVFDSH
CCCCCCHHHHCCCEEECCHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEECC
WLTTTEAVVTAHARRAGRFTSDEMPNAIRQLPYDLAGDPALAHAIAEQARRRASWIDAVD
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DPCLPLHYATLNPWTYLGRPDKRWLSVSVCQTATTEDFLRMGETVAQAIAQLERKVLLVA
CCCCEEEEEECCCCEECCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEE
SGGLSHAFWPLAELRGRMAGAASNIVTPAARAADERRIAWLEQGRHDRVIDAMPEFLRFA
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
PEANFGHYLMMAGALGARACAARARRFSEYENGIGTGHVHLWFSPADGGWARAETHNERR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHH
TETDAASA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]