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The map label for this gene is tolC [H]

Identifier: 76818773

GI number: 76818773

Start: 439125

End: 440555

Strand: Direct

Name: tolC [H]

Synonym: BURPS1710b_A0296

Alternate gene names: 76818773

Gene position: 439125-440555 (Clockwise)

Preceding gene: 76818020

Following gene: 76819335

Centisome position: 13.8

GC content: 65.62

Gene sequence:

>1431_bases
ATGTTTGAAACAGAGAATTTGGGTGCCTCGTCTAAATTTCGGCATGCGGGTTTTCACTTAACGACAAAATCTTTTCGGCT
TCGCGCCAATGTGGCCTACGTTACCGTTTGCGCGGTGCTTGCCGCACTCGGCACCGGTTCCGAATCCGCACGTGCGTTCT
GTCTCGACGAGGCTTATCAGCACGCAATATCGAACGATCCGAAATTTCTCCAGGCGCGGGCGGAATACGACGCCGCGCGG
CAGAAGTTTCCGCAGGCGCGCGCGCAGATGCTGCCGCAGGTGAGCGCGCAGCTCGAATGGGGCCGCTACGGCACGCACGC
GAACCTGTTCGGCATCGACGTGAGCGGCAACAGCACCGCCGCGTACGGCGCCGCGCAGCTCTCGCAGGCGCTGTTCAACA
TGCCGTATTTGTACGACATGAGCCGCGCGAAGGAATTCGAGGAATCCGCGCGGCAGCAGCTCGAAGTCGCGAAGCAGGAG
CTGATCATGCGCGTCGCGAACGCGTGCTTCGATCTGCTGTCCGCGCGCGAGAAGCTGCAGCTCGCCGACGACGAGGTCGG
CGCGCTCACGCGCCTGGAGAGCGATACCCGCCGCATGGCGCAGCTCGGCATGAAGACCATCGGCGACACGGCCGAGATCG
AGGCGCGCAGGAGCCTCGCGCAGTCGGACGAGGCGCTCGCGCGCACCGACGTCGAGGCGCGGCGCGCCCGCTACGAGACG
CTGCTCGGCTCCGCGATCGACTTCACGCGCTGGCCGCGGCTCGCGATGCACGGCACGTCGCCGCGCATTCCGACGGGCGA
CTACCAGCCGCAGGACAACCCGTCGTACCAGCAGGCGTATCGCGACCTGCGCGTCGCGCGGCTCGCGTCCAAGCGCATCA
ACGCGGAGCACCTGCCGAGCGTCGACCTGTTCGCGACGTACTCGCGCGGCCTCAATCCGAACCTGCGCGGCCTCACCGAC
AAGAACGACTTCCACCAGAGCGCGGTCGGCGTGCAGGTGACGATTCCGATCTTCTCGGGCGGCAGCGTGCACTACCGGAA
GATCGAGGCCGATCACGTCGCGACGCAGTACCAGAACCGGCTGCGCGAGGTCGAGCAGCAACTGAGCACCGATCATCGCG
AGACGCTCGCGGCGCTGCAGTCGATCGGCACGCGGATCCGCGCGCTGCAGCAATCGCTGCAGGCGGCGCGGCTCGCGTAC
GATTCGTCGATGAAGGCGCACCAGGTCGGCTACAGCACGACGTACGAGACGCTGAACCTGCGCACCGACATCTCGAACAT
CCGCCAGAAGCTGTTCGAGAGCTACCTCGACGCGCTGAAGCTCCAGCTGAAGCTCAAGGGCATTCTCGGCACGCTGGACG
AGCAGTCGCTCGTCGCGGTCGACAGCTTCCTCGCGAGCAACGCGGCGCCCGCCGATCAGAAGAGCGAATGA

Upstream 100 bases:

>100_bases
CCTGAATTCACCAAAAAGATGCATGCGTTTCAACTCGATATTTAAAACGTTTAATTTGTGTCCCGGACCACTTGACACAA
GGCAGGAGATGGAGACGGAG

Downstream 100 bases:

>100_bases
GCGTGTGACGGTGCGGCCGCACGGCGGCGTGGGCGTGACGCGCGCGAGCGCGTGGGAGAAGCCGGGCCGGCGCTCAAACC
GCAAGCGCGCCGAAAGCACG

Product: outer membrane protein TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MFETENLGASSKFRHAGFHLTTKSFRLRANVAYVTVCAVLAALGTGSESARAFCLDEAYQHAISNDPKFLQARAEYDAAR
QKFPQARAQMLPQVSAQLEWGRYGTHANLFGIDVSGNSTAAYGAAQLSQALFNMPYLYDMSRAKEFEESARQQLEVAKQE
LIMRVANACFDLLSAREKLQLADDEVGALTRLESDTRRMAQLGMKTIGDTAEIEARRSLAQSDEALARTDVEARRARYET
LLGSAIDFTRWPRLAMHGTSPRIPTGDYQPQDNPSYQQAYRDLRVARLASKRINAEHLPSVDLFATYSRGLNPNLRGLTD
KNDFHQSAVGVQVTIPIFSGGSVHYRKIEADHVATQYQNRLREVEQQLSTDHRETLAALQSIGTRIRALQQSLQAARLAY
DSSMKAHQVGYSTTYETLNLRTDISNIRQKLFESYLDALKLQLKLKGILGTLDEQSLVAVDSFLASNAAPADQKSE

Sequences:

>Translated_476_residues
MFETENLGASSKFRHAGFHLTTKSFRLRANVAYVTVCAVLAALGTGSESARAFCLDEAYQHAISNDPKFLQARAEYDAAR
QKFPQARAQMLPQVSAQLEWGRYGTHANLFGIDVSGNSTAAYGAAQLSQALFNMPYLYDMSRAKEFEESARQQLEVAKQE
LIMRVANACFDLLSAREKLQLADDEVGALTRLESDTRRMAQLGMKTIGDTAEIEARRSLAQSDEALARTDVEARRARYET
LLGSAIDFTRWPRLAMHGTSPRIPTGDYQPQDNPSYQQAYRDLRVARLASKRINAEHLPSVDLFATYSRGLNPNLRGLTD
KNDFHQSAVGVQVTIPIFSGGSVHYRKIEADHVATQYQNRLREVEQQLSTDHRETLAALQSIGTRIRALQQSLQAARLAY
DSSMKAHQVGYSTTYETLNLRTDISNIRQKLFESYLDALKLQLKLKGILGTLDEQSLVAVDSFLASNAAPADQKSE
>Mature_476_residues
MFETENLGASSKFRHAGFHLTTKSFRLRANVAYVTVCAVLAALGTGSESARAFCLDEAYQHAISNDPKFLQARAEYDAAR
QKFPQARAQMLPQVSAQLEWGRYGTHANLFGIDVSGNSTAAYGAAQLSQALFNMPYLYDMSRAKEFEESARQQLEVAKQE
LIMRVANACFDLLSAREKLQLADDEVGALTRLESDTRRMAQLGMKTIGDTAEIEARRSLAQSDEALARTDVEARRARYET
LLGSAIDFTRWPRLAMHGTSPRIPTGDYQPQDNPSYQQAYRDLRVARLASKRINAEHLPSVDLFATYSRGLNPNLRGLTD
KNDFHQSAVGVQVTIPIFSGGSVHYRKIEADHVATQYQNRLREVEQQLSTDHRETLAALQSIGTRIRALQQSLQAARLAY
DSSMKAHQVGYSTTYETLNLRTDISNIRQKLFESYLDALKLQLKLKGILGTLDEQSLVAVDSFLASNAAPADQKSE

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=446, Percent_Identity=27.5784753363229, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53056; Mature: 53056

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFETENLGASSKFRHAGFHLTTKSFRLRANVAYVTVCAVLAALGTGSESARAFCLDEAYQ
CCCCCCCCCCCCCCCCCEEEEECCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HAISNDPKFLQARAEYDAARQKFPQARAQMLPQVSAQLEWGRYGTHANLFGIDVSGNSTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCH
AYGAAQLSQALFNMPYLYDMSRAKEFEESARQQLEVAKQELIMRVANACFDLLSAREKLQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADDEVGALTRLESDTRRMAQLGMKTIGDTAEIEARRSLAQSDEALARTDVEARRARYET
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGSAIDFTRWPRLAMHGTSPRIPTGDYQPQDNPSYQQAYRDLRVARLASKRINAEHLPS
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
VDLFATYSRGLNPNLRGLTDKNDFHQSAVGVQVTIPIFSGGSVHYRKIEADHVATQYQNR
CHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCEEEEEECHHHHHHHHHHH
LREVEQQLSTDHRETLAALQSIGTRIRALQQSLQAARLAYDSSMKAHQVGYSTTYETLNL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEH
RTDISNIRQKLFESYLDALKLQLKLKGILGTLDEQSLVAVDSFLASNAAPADQKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFETENLGASSKFRHAGFHLTTKSFRLRANVAYVTVCAVLAALGTGSESARAFCLDEAYQ
CCCCCCCCCCCCCCCCCEEEEECCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
HAISNDPKFLQARAEYDAARQKFPQARAQMLPQVSAQLEWGRYGTHANLFGIDVSGNSTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCH
AYGAAQLSQALFNMPYLYDMSRAKEFEESARQQLEVAKQELIMRVANACFDLLSAREKLQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADDEVGALTRLESDTRRMAQLGMKTIGDTAEIEARRSLAQSDEALARTDVEARRARYET
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGSAIDFTRWPRLAMHGTSPRIPTGDYQPQDNPSYQQAYRDLRVARLASKRINAEHLPS
HHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
VDLFATYSRGLNPNLRGLTDKNDFHQSAVGVQVTIPIFSGGSVHYRKIEADHVATQYQNR
CHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCEEEEEECHHHHHHHHHHH
LREVEQQLSTDHRETLAALQSIGTRIRALQQSLQAARLAYDSSMKAHQVGYSTTYETLNL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHEEH
RTDISNIRQKLFESYLDALKLQLKLKGILGTLDEQSLVAVDSFLASNAAPADQKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]