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The map label for this gene is tfdA [H]

Identifier: 76818747

GI number: 76818747

Start: 160763

End: 161746

Strand: Direct

Name: tfdA [H]

Synonym: BURPS1710b_A0131

Alternate gene names: 76818747

Gene position: 160763-161746 (Clockwise)

Preceding gene: 76817622

Following gene: 76819768

Centisome position: 5.05

GC content: 57.72

Gene sequence:

>984_bases
ATGTATTCCGGGCGATATTTTTTTAATGCCTTTAATTCAATCAGGAATCCGATTTTCGATCGAATATCGAAAATAACGCC
GCATTCGGATTCCGATCGAAGCGCATCGCGTTCAAACATCGTCACCACAGGAATTCGCATGATTTCACGCAAATTGTCCC
CTGCGCTCGGCGCAGAGATTCGAGGCATCGATTTTTCTAAACCGCTGTCGTCGCAAGCGCGCGACGACGTCATCGGTTTG
TTGTCCGAACATCAATTGCTCGTCTTTCCCGGCCAGTGCCTGTCGTGCGAACAGCAGATCGCCGCGTGCGGCGCGTTCGG
CGAGCTCGAGCCGCACCCGATGACGACCAATACGTCCTCGTTCCCGGAAATGACGATCGTGTCGAACGTGACGTCGGACG
GCAAGCCGGTCGGCTATCCGACGCCGCCGTTCGAGCTGTGGCATTCGGATCTGTGCTATCTCGAGCACCCGGCGAAAATG
ACGTTCTTCTATGCCGAATCCGTGCCCGACGCGCACGGCGATACCTGGTTCGCGAACATGTTCCGCGCATACGAGACGCT
GCCCGACGAACTGAAAGCGGCGATCGACGGCAAGCATGCGGTCTTCAGTCTCGACAGCAGCCTCGTGAAGCGATGCAGGA
AGATCGGCTTCGATCTCAATATCGCGGAAGACGATTTCAAGCCGACCGTCTCGCATCCGGCGGTGCGCACCCATCCGCAC
ACGCGCCAACGCTCGATCTTCGTCAACTGGGCGCACACCGACCGGATCGAGGGCTATTCGCCCGAGGAAAGCGACGAGAT
TCTCGATCGCATCTTCGCGCACTGCCGCAACGAGGATTTCATCTACCGTCATCGCTACGCGAACGAAGACCTCGTGATCT
GGGACAACGCGTCGCTGATCCACACCAATTCGCCGAACCCGCCCGTCGGCAATCGCATCATGCGGCGCGTGATGGTGTCC
GGGCCGAAGCCGTTCTATCAGTAA

Upstream 100 bases:

>100_bases
CCCTCGATTTCACACCGTTTCCGGCATCCATTCTTCACGCCCATTTTCGATGCCGCGCCGATTCCCGATAAAGACCTGTG
ATAATTGATAGCTAGACAGC

Downstream 100 bases:

>100_bases
GTACCGGTCAATCCCAAGCGCCGAACGCGGCCGCCGGCAATCGCCCGGCGAGGCGGCCCCCTGAAGCCGACGCTTCGCCG
CTTGCCGGCGGCGGCCCACG

Product: TauD/TfdA family dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MYSGRYFFNAFNSIRNPIFDRISKITPHSDSDRSASRSNIVTTGIRMISRKLSPALGAEIRGIDFSKPLSSQARDDVIGL
LSEHQLLVFPGQCLSCEQQIAACGAFGELEPHPMTTNTSSFPEMTIVSNVTSDGKPVGYPTPPFELWHSDLCYLEHPAKM
TFFYAESVPDAHGDTWFANMFRAYETLPDELKAAIDGKHAVFSLDSSLVKRCRKIGFDLNIAEDDFKPTVSHPAVRTHPH
TRQRSIFVNWAHTDRIEGYSPEESDEILDRIFAHCRNEDFIYRHRYANEDLVIWDNASLIHTNSPNPPVGNRIMRRVMVS
GPKPFYQ

Sequences:

>Translated_327_residues
MYSGRYFFNAFNSIRNPIFDRISKITPHSDSDRSASRSNIVTTGIRMISRKLSPALGAEIRGIDFSKPLSSQARDDVIGL
LSEHQLLVFPGQCLSCEQQIAACGAFGELEPHPMTTNTSSFPEMTIVSNVTSDGKPVGYPTPPFELWHSDLCYLEHPAKM
TFFYAESVPDAHGDTWFANMFRAYETLPDELKAAIDGKHAVFSLDSSLVKRCRKIGFDLNIAEDDFKPTVSHPAVRTHPH
TRQRSIFVNWAHTDRIEGYSPEESDEILDRIFAHCRNEDFIYRHRYANEDLVIWDNASLIHTNSPNPPVGNRIMRRVMVS
GPKPFYQ
>Mature_327_residues
MYSGRYFFNAFNSIRNPIFDRISKITPHSDSDRSASRSNIVTTGIRMISRKLSPALGAEIRGIDFSKPLSSQARDDVIGL
LSEHQLLVFPGQCLSCEQQIAACGAFGELEPHPMTTNTSSFPEMTIVSNVTSDGKPVGYPTPPFELWHSDLCYLEHPAKM
TFFYAESVPDAHGDTWFANMFRAYETLPDELKAAIDGKHAVFSLDSSLVKRCRKIGFDLNIAEDDFKPTVSHPAVRTHPH
TRQRSIFVNWAHTDRIEGYSPEESDEILDRIFAHCRNEDFIYRHRYANEDLVIWDNASLIHTNSPNPPVGNRIMRRVMVS
GPKPFYQ

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=285, Percent_Identity=29.8245614035088, Blast_Score=133, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322971, Length=270, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 37071; Mature: 37071

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSGRYFFNAFNSIRNPIFDRISKITPHSDSDRSASRSNIVTTGIRMISRKLSPALGAEI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCEE
RGIDFSKPLSSQARDDVIGLLSEHQLLVFPGQCLSCEQQIAACGAFGELEPHPMTTNTSS
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHCHHHHHHHHCCCCCCCCCCCCCCCCC
FPEMTIVSNVTSDGKPVGYPTPPFELWHSDLCYLEHPAKMTFFYAESVPDAHGDTWFANM
CCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHH
FRAYETLPDELKAAIDGKHAVFSLDSSLVKRCRKIGFDLNIAEDDFKPTVSHPAVRTHPH
HHHHHCCHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
TRQRSIFVNWAHTDRIEGYSPEESDEILDRIFAHCRNEDFIYRHRYANEDLVIWDNASLI
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEE
HTNSPNPPVGNRIMRRVMVSGPKPFYQ
ECCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYSGRYFFNAFNSIRNPIFDRISKITPHSDSDRSASRSNIVTTGIRMISRKLSPALGAEI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCEE
RGIDFSKPLSSQARDDVIGLLSEHQLLVFPGQCLSCEQQIAACGAFGELEPHPMTTNTSS
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHCHHHHHHHHCCCCCCCCCCCCCCCCC
FPEMTIVSNVTSDGKPVGYPTPPFELWHSDLCYLEHPAKMTFFYAESVPDAHGDTWFANM
CCCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCEEEEEEECCCCCCCCCHHHHHH
FRAYETLPDELKAAIDGKHAVFSLDSSLVKRCRKIGFDLNIAEDDFKPTVSHPAVRTHPH
HHHHHCCHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
TRQRSIFVNWAHTDRIEGYSPEESDEILDRIFAHCRNEDFIYRHRYANEDLVIWDNASLI
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCEEE
HTNSPNPPVGNRIMRRVMVSGPKPFYQ
ECCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]