Click here to switch to the map view.

The map label for this gene is oprB [H]

Identifier: 76818680

GI number: 76818680

Start: 108412

End: 110067

Strand: Direct

Name: oprB [H]

Synonym: BURPS1710b_A0080

Alternate gene names: 76818680

Gene position: 108412-110067 (Clockwise)

Preceding gene: 76817978

Following gene: 76819231

Centisome position: 3.41

GC content: 72.22

Gene sequence:

>1656_bases
ATGGATCGCTTTCCTCACGATACGGCGGCGGCCTCGCGCCGTTGCCGCCGCCGCGCGGGCGGCGCCGCGCGCGCTTCGTG
CGCTTCGTGCGCCGCTTCGCGCGTCGCGTGCGCGTCGCTCGTGCGCACGGCGGTTCGCGCCGCCGCCGTCGCGGCCGCCT
TCTTCGCCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCGTCCGGCCGCGCCGGTGTCGGGCGCGTTCCCCGCCGACGGC
GTCTACGCCGCGCAGCCGGGCGCCGCGCCCGGCGCGCGCAGCGCGAACGGCCAGGCGGCCGTCGATATCGGCTGGCGCGA
GTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATCGCGCTGAAGAACAACCGCGACCTGCGCGTGTCGGTGCTCA
ACGTCGAGGCGTCGCGCGCGCAGTATCAGATCACGCGCGCGGGGCTCTTCCCGACGTTGAGCGGCACCGGCACGGGCACG
ATCCAGCGCACGCCGGCCGGCGTGTCGATCACCGGCCAGCCGCTCATCTCGCGGACCTACAACGTCGGCGTCTCCGCGTC
GTGGGAGCTCGACCTGTTCGGCCGCGTGCAGAGCCTGAAGGACCAGGCGCTCGCGCAATACTTCGCGACCGCGCAGGCGC
GCAAGGCCGCGGAGATCTCGCTCGTCGCGAGCGTCGCCGATCAGTACCTGACGCTGCTGTCGACCGACGATCTGCTGCAG
GTCACGCAGAACACGCTGAAGTCGGCGCGCGCGTCCTACGATCTGACGAAGCTGCAGTTCGACAACGGCACCGGCTCGGA
GCTCGACCTGCGCCAGGCGCAGACGGTGGTCGAGACCGCGCTCGCGAGCCAGCAGGCGCAGGCGCGCGCCCGCGCGCAGG
CGCTCAACGCGCTCGTGCTGCTGATCGGCGAGCCGCTGCCCGACGATCTGCCGGCCGGCCTGCCGCTCAATGCGCAGAAC
CTGCTCACCGACATTCCGGCCGGGCTGCCGTCCGATCTGCTCACGCGGCGGCCCGACATCATGCAGGCCGAGGAGACGCT
GCGCGCGGCGAACGCGAACATCGGCGCGGCGCGCGCGGCGTTCTTCCCGAAGATCTCGCTCACCAGCGCGTTCGGCACCG
CGAGCCCGACGCTCGGCGGCCTGTTCAAGGCGGGCACGGCGGCGTGGTCGTTCGCGCCGAACATCGCGCTGCCGATCTTC
GAGGGCGGGCAGAACATCGCGAACCTCGATCTCGCGCACGTGCAGAAGCGCATCGAGATCGCGAACTACGAGAAGGCGAT
CCAGAGCGCGTTTCGCGAGGTGTCGGACGGGCTTGCCGCGCGCGGCACGTACGATCAGCAGATCGCGGCGCTCGAGCGCA
ACGAGCACGCGCAGCAGCGCCGCTACGATCTGTCGGACCTGCGCTACAGGAACGGCGTCGACAGCTATCTGTCGGTGCTG
ACCGCGCAGACGGACCTGTATTCGGCGCAGCAGCAGTTGATCAGCGCGCGGCTCGCGCGCTGGACGAACCTCGTGGACCT
GTATCGCGCGCTGGGCGGCGGGTGGCTCGAGCGCGCGGGCGAGACGCCGCGCCCGGCGGACGCGCCCGTCGATTACGGCA
AGGCGGCGCCCGCGCCGGCGTCGGCATCGGCGCGAACAGCGGCCGTGACTGGTTGA

Upstream 100 bases:

>100_bases
TGCCGACCGTCTATACGTTCGTCGCGAAGGATCACGGCGCGGCGATGCGCAGCGAGCGCGCGCAACTGCTCGCCGCCACT
CGGTAACCAGGAACGACATG

Downstream 100 bases:

>100_bases
GCGCGCAACCCGACATGCGGCAACCCAACGCGCGGCAGCACAGCGCGCGGCGCCCGAGCGCAGCGCGCGCCGGCCGAGAG
CGAATCGGGCGCACCTGGCG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MDRFPHDTAAASRRCRRRAGGAARASCASCAASRVACASLVRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADG
VYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQ
VTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETALASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQN
LLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAAFFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIF
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVL
TAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAGETPRPADAPVDYGKAAPAPASASARTAAVTG

Sequences:

>Translated_551_residues
MDRFPHDTAAASRRCRRRAGGAARASCASCAASRVACASLVRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADG
VYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQ
VTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETALASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQN
LLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAAFFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIF
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVL
TAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAGETPRPADAPVDYGKAAPAPASASARTAAVTG
>Mature_551_residues
MDRFPHDTAAASRRCRRRAGGAARASCASCAASRVACASLVRTAVRAAAVAAAFFAAGCTLAPRYERPAAPVSGAFPADG
VYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQ
VTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETALASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQN
LLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAAFFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIF
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVL
TAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAGETPRPADAPVDYGKAAPAPASASARTAAVTG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=41.703056768559, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI2367348, Length=425, Percent_Identity=24.7058823529412, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87082199, Length=315, Percent_Identity=23.4920634920635, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 58453; Mature: 58453

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFPHDTAAASRRCRRRAGGAARASCASCAASRVACASLVRTAVRAAAVAAAFFAAGCT
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHH
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTY
HHCCCCCEEEEEEEEECCHHHEEEEHHCCCCCCCCCCCCEEEECCCCCEECCCHHHHHHE
NVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQ
ECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETALASQQAQARARAQALNALVL
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
EECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHH
FFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEI
HCCCEEHHHHCCCCCCCHHHHHHHCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
ANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVL
HHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHH
TAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAGETPRPADAPVDYGKAAPAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
SASARTAAVTG
CCCCCCEECCC
>Mature Secondary Structure
MDRFPHDTAAASRRCRRRAGGAARASCASCAASRVACASLVRTAVRAAAVAAAFFAAGCT
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCHHHHHCCHHHHHHHHH
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTY
HHCCCCCEEEEEEEEECCHHHEEEEHHCCCCCCCCCCCCEEEECCCCCEECCCHHHHHHE
NVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQ
ECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETALASQQAQARARAQALNALVL
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
EECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHH
FFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEI
HCCCEEHHHHCCCCCCCHHHHHHHCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
ANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVL
HHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHH
TAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAGETPRPADAPVDYGKAAPAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
SASARTAAVTG
CCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]