Click here to switch to the map view.

The map label for this gene is tms2 [H]

Identifier: 76818487

GI number: 76818487

Start: 1544646

End: 1546049

Strand: Direct

Name: tms2 [H]

Synonym: BURPS1710b_A1234

Alternate gene names: 76818487

Gene position: 1544646-1546049 (Clockwise)

Preceding gene: 76817726

Following gene: 76817734

Centisome position: 48.55

GC content: 73.15

Gene sequence:

>1404_bases
ATGACGTCACATCGGGATACCTACGTCCGGTCCGCCAGCGTGCGAGCCATCGCGGATGCCGTGAACGGGGGGCGGGTGCG
CGCGCTCGAGCAGGCCGAGGCGGCGCTCGAACGTCTCGCGCTCGTCGAGCCGCTCGCGGCGATGACGCACGTCGCGCCGG
AGATCGCGCGCCGGGCCGCCGCGAACGTCGACGCGCAGATCGCGAGCGGCCGCCGGCTGCCGCTCGCGGGCGTGCCGTTC
GTCATCAAGGACAACCTCTTCACCGCCGACATGCCGACGCGCGCGGCCAGCCCCGCGCTGCTCGGCTTCGACGCGACGCG
CAACGCGACGGCCGTCCAGCAGTTGCTCGACGCGGGCGCCGTGCCGCTCGGCAAGGCGAACATGCACGAGCTCGCGTTCG
GCATCACGAGCGCGAACGGCTACTTCGGCGCGGTGCGTAATCCGCACGACCCGGCGCGCATCGCGGGCGGCTCGAGCGGC
GGCACGGCGTGCGCGGTCGCGGCGGGCATCGCGTTCGGCCTCGGCACCGACACGGGCGGCTCGGTGCGCATCCCGGCCGC
GTTTTGCGGCGTCGCCGGATTGCGGCCGACGCCGCGCCGCTACTCGACGGAAGGCGTGCTCGCGCTATCGCCGACGCGCG
ATACGGTCGGCCCGATCGCGCACGGCATCGACGATTTGCTGCTGCTCGACGCCGTGCTGACGGGCGACGCCGGCGAGCCC
GCACCGGTCGCGCTCGCCGGCTTGCGCGTCGGCGTGCCGGATGCGCCCTACTTCGACGGGCTCGACCCGGCCGTCGCGGA
ACTGACCGAGCGCGCACTCGCCGCGCTGACGGACGCGGGCGCGCGACTGATCCCGCTCGCCGCCCACGCGCTGCACGAAG
CGGACGAAGCCTGCGGCTTCCCGATCGCCGGGTTCGAGGCGCTCGCGTACTGGCGCCGCTTCAGCGCCGAACGGCTCGAT
CTGCCGTTCGCGGAATTCGTCGCCCGGCTCGGCAGCGACGATGTGCGCCACCTCTTCCGCATGCTGATCGACGCACCGCC
GTCGAGCGATGCGTATGCGGCCGCGATCGACGTGCTGCAAAGCACCGTGCGCTGGTACGACCAGGCGTTCCATCGGACGG
GCGTCGATTGCATCGCGCTGCCGACCGTTCAATGCGACGCGCCGCTCGTCACCGACAGCGGGCACGATCTTCCTTCCGAC
GCCGCGTACGCGCTGTTCACGCGCGTGATCCGCCAGACCGGGCCGGCGACGCTCGGCGGCGTGCCCTCGGTATCGATTCC
GGCGGGATTGTCCAAGCACACCGGCATGCCCGTGGGCCTGATGCTCGAAGGGCCGGCAGGCAACGACCGGCGCCTGCTCG
CGATCGCGCGCGAGGTGGAGCGGCTGCTCGCGCGGGCGCCGTGA

Upstream 100 bases:

>100_bases
ATCCATTCGTCGGGATGGCCGCCGGAAGGGCCGATCGAAGCGACAACGCATGCCGCACGCCGCGCGTCGCGGCGCGCCAC
ACACATCAGGAGACAAGTCA

Downstream 100 bases:

>100_bases
AGCGCGCGTGCTTGCCTGGCGCTCGTGTGACGCTCATCCGAGGTTCATTTGACGCTCGTGTGACGCGCCTGTGGTGCTCG
CCCCGCGCGGGTCTCGCGCA

Product: indole acetimide hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTSHRDTYVRSASVRAIADAVNGGRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPF
VIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSG
GTACAVAAGIAFGLGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEP
APVALAGLRVGVPDAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLD
LPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSD
AAYALFTRVIRQTGPATLGGVPSVSIPAGLSKHTGMPVGLMLEGPAGNDRRLLAIAREVERLLARAP

Sequences:

>Translated_467_residues
MTSHRDTYVRSASVRAIADAVNGGRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPF
VIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSG
GTACAVAAGIAFGLGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEP
APVALAGLRVGVPDAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLD
LPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSD
AAYALFTRVIRQTGPATLGGVPSVSIPAGLSKHTGMPVGLMLEGPAGNDRRLLAIAREVERLLARAP
>Mature_466_residues
TSHRDTYVRSASVRAIADAVNGGRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAAANVDAQIASGRRLPLAGVPFV
IKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGAVPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGG
TACAVAAGIAFGLGTDTGGSVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEPA
PVALAGLRVGVPDAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGFPIAGFEALAYWRRFSAERLDL
PFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQSTVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDA
AYALFTRVIRQTGPATLGGVPSVSIPAGLSKHTGMPVGLMLEGPAGNDRRLLAIAREVERLLARAP

Specific function: Hydrolyzes both the R- and the S-enantiomers of mandelamide, and phenylacetamide. Has lower activity on 3- phenylpropionaide and lactamide. Does not hydrolyze benzamide. Hydrolyzes esters and amides with little steric bulk. Preferentially hydrolyzes aroma

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=29.3650793650794, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI195972892, Length=233, Percent_Identity=30.4721030042918, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=470, Percent_Identity=27.2340425531915, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=235, Percent_Identity=31.063829787234, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=451, Percent_Identity=29.0465631929047, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=231, Percent_Identity=29.4372294372294, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=157, Percent_Identity=37.5796178343949, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=157, Percent_Identity=37.5796178343949, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=228, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=439, Percent_Identity=27.5626423690205, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319685, Length=236, Percent_Identity=31.7796610169492, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=30.8467741935484, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21356731, Length=495, Percent_Identity=28.4848484848485, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI45550774, Length=480, Percent_Identity=25.8333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=480, Percent_Identity=25.8333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648437, Length=480, Percent_Identity=25.8333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=26.0956175298805, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=26.0956175298805, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=26.0956175298805, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=26.0956175298805, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644968, Length=488, Percent_Identity=23.7704918032787, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648441, Length=414, Percent_Identity=25.8454106280193, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648439, Length=414, Percent_Identity=25.8454106280193, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI161078093, Length=503, Percent_Identity=25.2485089463221, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.86 [H]

Molecular weight: Translated: 48509; Mature: 48377

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHRDTYVRSASVRAIADAVNGGRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANVDAQIASGRRLPLAGVPFVIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGA
CCCCHHHHCCCCCCCCCCCEEEECCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCC
VPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFGLGTDTGG
CCCCCCCHHHHHEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
SVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEP
CEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
APVALAGLRVGVPDAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGF
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PIAGFEALAYWRRFSAERLDLPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQ
CCHHHHHHHHHHHHCHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
STVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFTRVIRQTGPATLGG
HHHHHHHHHHHHCCCCEEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPSVSIPAGLSKHTGMPVGLMLEGPAGNDRRLLAIAREVERLLARAP
CCCCCCCCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSHRDTYVRSASVRAIADAVNGGRVRALEQAEAALERLALVEPLAAMTHVAPEIARRAA
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANVDAQIASGRRLPLAGVPFVIKDNLFTADMPTRAASPALLGFDATRNATAVQQLLDAGA
CCCCHHHHCCCCCCCCCCCEEEECCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCC
VPLGKANMHELAFGITSANGYFGAVRNPHDPARIAGGSSGGTACAVAAGIAFGLGTDTGG
CCCCCCCHHHHHEEEECCCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
SVRIPAAFCGVAGLRPTPRRYSTEGVLALSPTRDTVGPIAHGIDDLLLLDAVLTGDAGEP
CEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
APVALAGLRVGVPDAPYFDGLDPAVAELTERALAALTDAGARLIPLAAHALHEADEACGF
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PIAGFEALAYWRRFSAERLDLPFAEFVARLGSDDVRHLFRMLIDAPPSSDAYAAAIDVLQ
CCHHHHHHHHHHHHCHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
STVRWYDQAFHRTGVDCIALPTVQCDAPLVTDSGHDLPSDAAYALFTRVIRQTGPATLGG
HHHHHHHHHHHHCCCCEEEECCEECCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPSVSIPAGLSKHTGMPVGLMLEGPAGNDRRLLAIAREVERLLARAP
CCCCCCCCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12670968 [H]