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The map label for this gene is potH [H]

Identifier: 76818369

GI number: 76818369

Start: 1923512

End: 1924429

Strand: Direct

Name: potH [H]

Synonym: BURPS1710b_A1579

Alternate gene names: 76818369

Gene position: 1923512-1924429 (Clockwise)

Preceding gene: 76819491

Following gene: 76817648

Centisome position: 60.45

GC content: 56.21

Gene sequence:

>918_bases
GTGATTTTTCGAGTTCCGACTCTAGAGAAGTTTAAATCATGGCGAGCGGTTTACGAACCGGCCTCGCTTGTCATGATCTT
AGGCGTGATATTCCCTCTCGCGGTTCTAGCGGTCATATCGTTGAGCGCACCCCTCGATTTCGGCGGAGTTTCGTGGGGGG
ATTTCACGATTCGCGCCTATGAGCGCGTAGTGTTTGATCATGATTGGGACGGCTCGCTGGTACTTCAACAAGGCTACGTG
AGCATCTTTCTACGATCTGCGACCATGGCGTTACTGGCGACTTCCGGGTGCGTCCTCCTCGGGTTTCCCGTCGCGCTATT
TATCGCAACCCGTGCACCGACTTGGAGGGCCGTCTTGTTGGTGCTCGTGACCATACCGTTCTGGACCTGCGTCGTGGTGC
AAATGTGCGGCTGGATTATCGTCATGGCGGACAATGGTTTACTTAACCGTGTCCTTGCCGTGTTAGGTATCCTTCGAGGA
CCGCTCGGATTACTTTACACCGACATCGCAACTCTGGTCGGCCTCGTCTATGCATTCACCCCCTTTATGGTTTTGCCGAT
TTTCGCGGCTCTTGACGAATTTGACTGGAGCCTGGTCGAGGCTGCTTACGATCTTGGCGCCAATAGGGGAGCGGCGCTTC
TGCAGGTGATCGTTCCTGGCTGTCGTTCCGGCATCATGGCTGGCATCGGGCTCGTTTTCGTTCCAGCGCTCGGTTCGTAT
GCGATCCCTAGCTTGCTTGGCGGCAACCACGCATTCATGGTCGGGAACCTGATCGATTTTCAATTCGCAGGGGGCCGCGA
TTGGCCGTTGGGCGCGGCTATGTCGTTTGTCCTACTCGCTGTCGTTCTTCTCGCCATGCTCGCGCTGCGTCTGACATCCA
TCCTCCAGCGACAGGAGCTCGCTCATGACGTCGCGTGA

Upstream 100 bases:

>100_bases
AGATAACAGATTGGCGCGTTCAGCACGCATGTGCTCTATAAATATGGCGGCCAATTTGTTACGCTTTCCCTCCGGTAAGC
TGATCGATTCGAGGTAATCG

Downstream 100 bases:

>100_bases
ACTGCGCCACTTTCCGCTTACGGCAGAAGTCGCGATTGCCGTCCTGTTGTTTCTCTATCTGCCGTTGGCGGTTGTCGCGT
GGATGAGTTTCAATCGAGGC

Product: spermidine/putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MIFRVPTLEKFKSWRAVYEPASLVMILGVIFPLAVLAVISLSAPLDFGGVSWGDFTIRAYERVVFDHDWDGSLVLQQGYV
SIFLRSATMALLATSGCVLLGFPVALFIATRAPTWRAVLLVLVTIPFWTCVVVQMCGWIIVMADNGLLNRVLAVLGILRG
PLGLLYTDIATLVGLVYAFTPFMVLPIFAALDEFDWSLVEAAYDLGANRGAALLQVIVPGCRSGIMAGIGLVFVPALGSY
AIPSLLGGNHAFMVGNLIDFQFAGGRDWPLGAAMSFVLLAVVLLAMLALRLTSILQRQELAHDVA

Sequences:

>Translated_305_residues
MIFRVPTLEKFKSWRAVYEPASLVMILGVIFPLAVLAVISLSAPLDFGGVSWGDFTIRAYERVVFDHDWDGSLVLQQGYV
SIFLRSATMALLATSGCVLLGFPVALFIATRAPTWRAVLLVLVTIPFWTCVVVQMCGWIIVMADNGLLNRVLAVLGILRG
PLGLLYTDIATLVGLVYAFTPFMVLPIFAALDEFDWSLVEAAYDLGANRGAALLQVIVPGCRSGIMAGIGLVFVPALGSY
AIPSLLGGNHAFMVGNLIDFQFAGGRDWPLGAAMSFVLLAVVLLAMLALRLTSILQRQELAHDVA
>Mature_305_residues
MIFRVPTLEKFKSWRAVYEPASLVMILGVIFPLAVLAVISLSAPLDFGGVSWGDFTIRAYERVVFDHDWDGSLVLQQGYV
SIFLRSATMALLATSGCVLLGFPVALFIATRAPTWRAVLLVLVTIPFWTCVVVQMCGWIIVMADNGLLNRVLAVLGILRG
PLGLLYTDIATLVGLVYAFTPFMVLPIFAALDEFDWSLVEAAYDLGANRGAALLQVIVPGCRSGIMAGIGLVFVPALGSY
AIPSLLGGNHAFMVGNLIDFQFAGGRDWPLGAAMSFVLLAVVLLAMLALRLTSILQRQELAHDVA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=209, Percent_Identity=41.1483253588517, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1787369, Length=241, Percent_Identity=36.9294605809129, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787713, Length=218, Percent_Identity=28.8990825688073, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32907; Mature: 32907

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFRVPTLEKFKSWRAVYEPASLVMILGVIFPLAVLAVISLSAPLDFGGVSWGDFTIRAY
CEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ERVVFDHDWDGSLVLQQGYVSIFLRSATMALLATSGCVLLGFPVALFIATRAPTWRAVLL
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
VLVTIPFWTCVVVQMCGWIIVMADNGLLNRVLAVLGILRGPLGLLYTDIATLVGLVYAFT
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFMVLPIFAALDEFDWSLVEAAYDLGANRGAALLQVIVPGCRSGIMAGIGLVFVPALGSY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AIPSLLGGNHAFMVGNLIDFQFAGGRDWPLGAAMSFVLLAVVLLAMLALRLTSILQRQEL
HHHHHHCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHDVA
HHCCC
>Mature Secondary Structure
MIFRVPTLEKFKSWRAVYEPASLVMILGVIFPLAVLAVISLSAPLDFGGVSWGDFTIRAY
CEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ERVVFDHDWDGSLVLQQGYVSIFLRSATMALLATSGCVLLGFPVALFIATRAPTWRAVLL
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHH
VLVTIPFWTCVVVQMCGWIIVMADNGLLNRVLAVLGILRGPLGLLYTDIATLVGLVYAFT
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFMVLPIFAALDEFDWSLVEAAYDLGANRGAALLQVIVPGCRSGIMAGIGLVFVPALGSY
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AIPSLLGGNHAFMVGNLIDFQFAGGRDWPLGAAMSFVLLAVVLLAMLALRLTSILQRQEL
HHHHHHCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHDVA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]