Click here to switch to the map view.

The map label for this gene is potF [H]

Identifier: 76818255

GI number: 76818255

Start: 1924940

End: 1926298

Strand: Direct

Name: potF [H]

Synonym: BURPS1710b_A1581

Alternate gene names: 76818255

Gene position: 1924940-1926298 (Clockwise)

Preceding gene: 76817648

Following gene: 76819042

Centisome position: 60.5

GC content: 53.94

Gene sequence:

>1359_bases
ATGGGCGGCATTTCGTCAAATCACGGTTCCTTTGGCATTGCCCGGTATCGTCGCCGGTGCGCTGCTTGCGTTCGTCGCGT
CTATGAACGACTACGTCACCAGCTACTTCTTGGCTGGTGCCGGCATGACCACCTTGCCTATGTACATCTTCAGCGCATTG
AAGATCGGAATTACGCCAAAGGTAAATGCTATCTCGACAGCCGTCATTGGACTTTCGTCGATTCAACTACTGACGGTCTG
GGTTCTCGGCAGGAAGGGTGCAGGACAGACTGCTACGCCGGGGAGATCCGAGCTGGGTCACGCGAACATTCAAACGATAG
GAACCGTATGAAAAAAGTAGTCGGAGCGATGCTGCTGCTTGCTGTCGGTCATGCTGTCTCAGCCGAACTCCATATCGGAA
GCTGGCCGGATTACCTGCCCGATACGCTGATCAAGAAGTTCCAGGCGGAAACCGGCATCAAGACAACGCTCGACACCTAC
GCGAGCGATGCGGCCCTCACTCAAAAGTTGCAGTCGGGCGGAGGTGGCTACGACGTTGTGATCGCAGGGGACTATTACGT
TCCGGTGCTTGTGAAGTCTGGATTGCTGCAGAAGCTCGACAAGAACAAGCTGCCGAATATCGCCAACATCAAACCCGAGT
ATCGCCATCCGTCATTCGATCCGCAACGGGATTATGCGATGCCATACACCGTCGTGCTGACTGGTTTCGCGTACGATAGC
GCGCGCGTTTCCGGAGGGAAACTCGACGAGAGTTGGAAGTCGTTCTTCGATCCGCCAGCGCAGTTGCGCGGACAGATCGG
CGACCTGGATGTCGAAGAGGAGCTGTATATGGCCGCGAGTTGGTATCTGGGACAGGATGAATGCACAGAAAATCCTGCTG
ATGCGAAGCGGGTGCTCGACGTTTTGCAAAAACAGAAACCGTTCGTGAAAACGTACAGCAATGACGGAACCATAGATCGT
CTCGCGTCCAAGCAGATCGCGGTGCAGCATATTTGGAGCGGTGCCGCTGCGCGTGCACAGGACCGTCTTCCCAGCATCAC
CTTTGTATACCCGAGGGAAGGCGTCCGTCTGTTCATGGATAGCTTGCTTATTCCGGCAAAGGCACAAAACACCGCCTCGG
CATATCAATTCGTTAACTGGATGATGCGCCCGGAGAACATTGCGCAGGTTACAAATGCCGTTCGCTACAACAACGAGATC
ATCGGCTCCGAGCGGTATATCGATGCCGCACTGTTGAAAAACTCCGCGATAAAAACCCCGGAGCAATACAAAATAAGGCT
CCGTCCTTACAAAATATGTTCGCCGGCGGCGATTCAGTTACGAAACAAAGTCTGGCTAAAGTTGAAGGGAAATCGCTGA

Upstream 100 bases:

>100_bases
TGGTGTTTTGTGTCCCACTCGCGTATCTCCCGATCAACGCACGTCTTAAGACAATCGATCGGACATTACTTGAGGCGGCG
GCCGACTTGTCGGCTCCGCC

Downstream 100 bases:

>100_bases
CACACCACAGACTCAACCAGTTCCAAACCGGCGAATAGTCGAAGGAAAGAAAAATACAAGCTTAAGACGGCATGCCTGGT
TGTTTTGGAAAAGGCGGTCG

Product: putative ABC transporter exported substrate-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MGGISSNHGSFGIARYRRRCAACVRRVYERLRHQLLLGWCRHDHLAYVHLQRIEDRNYAKGKCYLDSRHWTFVDSTTDGL
GSRQEGCRTDCYAGEIRAGSREHSNDRNRMKKVVGAMLLLAVGHAVSAELHIGSWPDYLPDTLIKKFQAETGIKTTLDTY
ASDAALTQKLQSGGGGYDVVIAGDYYVPVLVKSGLLQKLDKNKLPNIANIKPEYRHPSFDPQRDYAMPYTVVLTGFAYDS
ARVSGGKLDESWKSFFDPPAQLRGQIGDLDVEEELYMAASWYLGQDECTENPADAKRVLDVLQKQKPFVKTYSNDGTIDR
LASKQIAVQHIWSGAAARAQDRLPSITFVYPREGVRLFMDSLLIPAKAQNTASAYQFVNWMMRPENIAQVTNAVRYNNEI
IGSERYIDAALLKNSAIKTPEQYKIRLRPYKICSPAAIQLRNKVWLKLKGNR

Sequences:

>Translated_452_residues
MGGISSNHGSFGIARYRRRCAACVRRVYERLRHQLLLGWCRHDHLAYVHLQRIEDRNYAKGKCYLDSRHWTFVDSTTDGL
GSRQEGCRTDCYAGEIRAGSREHSNDRNRMKKVVGAMLLLAVGHAVSAELHIGSWPDYLPDTLIKKFQAETGIKTTLDTY
ASDAALTQKLQSGGGGYDVVIAGDYYVPVLVKSGLLQKLDKNKLPNIANIKPEYRHPSFDPQRDYAMPYTVVLTGFAYDS
ARVSGGKLDESWKSFFDPPAQLRGQIGDLDVEEELYMAASWYLGQDECTENPADAKRVLDVLQKQKPFVKTYSNDGTIDR
LASKQIAVQHIWSGAAARAQDRLPSITFVYPREGVRLFMDSLLIPAKAQNTASAYQFVNWMMRPENIAQVTNAVRYNNEI
IGSERYIDAALLKNSAIKTPEQYKIRLRPYKICSPAAIQLRNKVWLKLKGNR
>Mature_451_residues
GGISSNHGSFGIARYRRRCAACVRRVYERLRHQLLLGWCRHDHLAYVHLQRIEDRNYAKGKCYLDSRHWTFVDSTTDGLG
SRQEGCRTDCYAGEIRAGSREHSNDRNRMKKVVGAMLLLAVGHAVSAELHIGSWPDYLPDTLIKKFQAETGIKTTLDTYA
SDAALTQKLQSGGGGYDVVIAGDYYVPVLVKSGLLQKLDKNKLPNIANIKPEYRHPSFDPQRDYAMPYTVVLTGFAYDSA
RVSGGKLDESWKSFFDPPAQLRGQIGDLDVEEELYMAASWYLGQDECTENPADAKRVLDVLQKQKPFVKTYSNDGTIDRL
ASKQIAVQHIWSGAAARAQDRLPSITFVYPREGVRLFMDSLLIPAKAQNTASAYQFVNWMMRPENIAQVTNAVRYNNEII
GSERYIDAALLKNSAIKTPEQYKIRLRPYKICSPAAIQLRNKVWLKLKGNR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=358, Percent_Identity=29.3296089385475, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787367, Length=329, Percent_Identity=28.8753799392097, Blast_Score=154, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 51065; Mature: 50934

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGISSNHGSFGIARYRRRCAACVRRVYERLRHQLLLGWCRHDHLAYVHLQRIEDRNYAK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCC
GKCYLDSRHWTFVDSTTDGLGSRQEGCRTDCYAGEIRAGSREHSNDRNRMKKVVGAMLLL
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVGHAVSAELHIGSWPDYLPDTLIKKFQAETGIKTTLDTYASDAALTQKLQSGGGGYDVV
HHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
IAGDYYVPVLVKSGLLQKLDKNKLPNIANIKPEYRHPSFDPQRDYAMPYTVVLTGFAYDS
EECCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ARVSGGKLDESWKSFFDPPAQLRGQIGDLDVEEELYMAASWYLGQDECTENPADAKRVLD
CCCCCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHH
VLQKQKPFVKTYSNDGTIDRLASKQIAVQHIWSGAAARAQDRLPSITFVYPREGVRLFMD
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCHHHHHHH
SLLIPAKAQNTASAYQFVNWMMRPENIAQVTNAVRYNNEIIGSERYIDAALLKNSAIKTP
HHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEECCCHHHHHHHHHHCCCCCCC
EQYKIRLRPYKICSPAAIQLRNKVWLKLKGNR
HHEEEEECCEECCCCHHHEECCEEEEEEECCC
>Mature Secondary Structure 
GGISSNHGSFGIARYRRRCAACVRRVYERLRHQLLLGWCRHDHLAYVHLQRIEDRNYAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCCC
GKCYLDSRHWTFVDSTTDGLGSRQEGCRTDCYAGEIRAGSREHSNDRNRMKKVVGAMLLL
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVGHAVSAELHIGSWPDYLPDTLIKKFQAETGIKTTLDTYASDAALTQKLQSGGGGYDVV
HHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
IAGDYYVPVLVKSGLLQKLDKNKLPNIANIKPEYRHPSFDPQRDYAMPYTVVLTGFAYDS
EECCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ARVSGGKLDESWKSFFDPPAQLRGQIGDLDVEEELYMAASWYLGQDECTENPADAKRVLD
CCCCCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHH
VLQKQKPFVKTYSNDGTIDRLASKQIAVQHIWSGAAARAQDRLPSITFVYPREGVRLFMD
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCHHHHHHH
SLLIPAKAQNTASAYQFVNWMMRPENIAQVTNAVRYNNEIIGSERYIDAALLKNSAIKTP
HHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEECCCHHHHHHHHHHCCCCCCC
EQYKIRLRPYKICSPAAIQLRNKVWLKLKGNR
HHEEEEECCEECCCCHHHEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]