Click here to switch to the map view.

The map label for this gene is hemL [H]

Identifier: 76818100

GI number: 76818100

Start: 1600421

End: 1601779

Strand: Direct

Name: hemL [H]

Synonym: BURPS1710b_A1290

Alternate gene names: 76818100

Gene position: 1600421-1601779 (Clockwise)

Preceding gene: 76818917

Following gene: 76819838

Centisome position: 50.3

GC content: 71.16

Gene sequence:

>1359_bases
TTGGAGATTCGCCACGGAATCAACCTGGCGCGCGCCCGCGCGCTTCTCGCACGCGAGCGCGACGCGTTCGAACGGGCGAT
GCCGAAATCGCGCGCGCGCTCGGCCGAAGCCGCGCAACATCTGTTGTTCGGCGTGCCGCTGCACTGGATGCGCGACTGGT
CGACGCCGTTCTCGCTCTATGTCGATCACGCGCGCGGCGCGCATTTCACCGACATCGACGGCCATCGCTACGCCGACTTC
TGCCTGGGCGACACGGGCGCGATGTTCGGCCACGCGCCCGAGCCCGTCGCACGCGCGCTCGCCGAGCAGGCGGCGCGCGG
CTACACGACGATGCTGCCGAGCGAGGACGCCGCGTGGGTCGCGGCGGAGCTCGCGCGCCGCTTCCGGCTGCCGTACTGGC
AGTTCGCGCTGAGCGCGAGCGACGCGAACCGCTTCGTGCTGCGCTGGGCGCGCGCGGCCACGGGCCGCAAGCACATCGTC
GTGTTCAACGGCTGCTATCACGGCACCGTCGACGACGTGTTCGTCGATCTCGCAGACGGCCGGCCGGTGCAGCGCGACAG
CCTGCTCGGCCAGGCGCACGATCTGTCGGCATACACGCGCGTCGTCGAATTCAACGATCTCGCCGCGCTCGAAGCCGCGC
TGAAGGACGGCGAGGTCGCATGCGTGCTCGCCGAGCCGGCGCTGACGAACATCGGCATGGTGCTGCCCGACGCCGGCTTC
TGGCGCGAAGCGCGCGCGCTCACGCGCCGCCACGGCACGCTGCTCGCGATCGACGAAACCCATACGATCAGCAGCGGCCC
CGGCGGCTATGCGCTCGCGCACGGGCTCGAGCCGGATCTGCTCGTCGTCGGCAAGCCGATCGGCGGCGGCGTGCCGTGCG
CGGTGTACGGCTTCAGCGCGTCGTGCGCCGAGCGCGCGCAGCACGCGAAGGCGAGCGCGCCGCCCGGCCATTCGGGCATC
GGCACGACGCTGACCGCGAACATGCTGGCGATGCGCGCGATCCGCGCGACGCTCGCCGACGTGATGACCGATGCCGCGTA
CGCGCACATGTTCGACCTCGCCGCACGGCTCGCGGCCGGGCTCGAACAGACGATCGCGCGACGCGGGCTGCCATGGTGCG
TGACGCGGATCGGCGCGCGCACCGAGTTCCAGTTCGCGCCGGCGCCGCCGCGCAACGGCACGATCGCCGGCCTGCAGCTC
GACGGCGAGCTCGAGCACATCGTGCACCTGTATCTGCTGAACCGCGGCGTGCTGATCACGCCGTTCCACAACATGATGCT
CGTGTGTCCGCAGACGAGCGCGGAAGACGTCGATCGGCTGGTGGCCGCATTCGACGCGTGCCTCGGCGAGCTGCTCTGA

Upstream 100 bases:

>100_bases
CGCTCACGATCGCTCACGGCCGGATCGACCAATCCGAACGAATATGCGAAGCTTTCGCCGACGTTCCGCGATCCCGTTTC
ATCCGCACAAGGAGCCCGTC

Downstream 100 bases:

>100_bases
CCGACATTGCGGCGGCGGCGCGAGCACGAACCTGCGAAGCGAACGAAGGAGGCGAAGCAGGCGAACGAGCGGATGCCGCA
TACGCCGGCGCAGGCGATAC

Product: aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEIRHGINLARARALLARERDAFERAMPKSRARSAEAAQHLLFGVPLHWMRDWSTPFSLYVDHARGAHFTDIDGHRYADF
CLGDTGAMFGHAPEPVARALAEQAARGYTTMLPSEDAAWVAAELARRFRLPYWQFALSASDANRFVLRWARAATGRKHIV
VFNGCYHGTVDDVFVDLADGRPVQRDSLLGQAHDLSAYTRVVEFNDLAALEAALKDGEVACVLAEPALTNIGMVLPDAGF
WREARALTRRHGTLLAIDETHTISSGPGGYALAHGLEPDLLVVGKPIGGGVPCAVYGFSASCAERAQHAKASAPPGHSGI
GTTLTANMLAMRAIRATLADVMTDAAYAHMFDLAARLAAGLEQTIARRGLPWCVTRIGARTEFQFAPAPPRNGTIAGLQL
DGELEHIVHLYLLNRGVLITPFHNMMLVCPQTSAEDVDRLVAAFDACLGELL

Sequences:

>Translated_452_residues
MEIRHGINLARARALLARERDAFERAMPKSRARSAEAAQHLLFGVPLHWMRDWSTPFSLYVDHARGAHFTDIDGHRYADF
CLGDTGAMFGHAPEPVARALAEQAARGYTTMLPSEDAAWVAAELARRFRLPYWQFALSASDANRFVLRWARAATGRKHIV
VFNGCYHGTVDDVFVDLADGRPVQRDSLLGQAHDLSAYTRVVEFNDLAALEAALKDGEVACVLAEPALTNIGMVLPDAGF
WREARALTRRHGTLLAIDETHTISSGPGGYALAHGLEPDLLVVGKPIGGGVPCAVYGFSASCAERAQHAKASAPPGHSGI
GTTLTANMLAMRAIRATLADVMTDAAYAHMFDLAARLAAGLEQTIARRGLPWCVTRIGARTEFQFAPAPPRNGTIAGLQL
DGELEHIVHLYLLNRGVLITPFHNMMLVCPQTSAEDVDRLVAAFDACLGELL
>Mature_452_residues
MEIRHGINLARARALLARERDAFERAMPKSRARSAEAAQHLLFGVPLHWMRDWSTPFSLYVDHARGAHFTDIDGHRYADF
CLGDTGAMFGHAPEPVARALAEQAARGYTTMLPSEDAAWVAAELARRFRLPYWQFALSASDANRFVLRWARAATGRKHIV
VFNGCYHGTVDDVFVDLADGRPVQRDSLLGQAHDLSAYTRVVEFNDLAALEAALKDGEVACVLAEPALTNIGMVLPDAGF
WREARALTRRHGTLLAIDETHTISSGPGGYALAHGLEPDLLVVGKPIGGGVPCAVYGFSASCAERAQHAKASAPPGHSGI
GTTLTANMLAMRAIRATLADVMTDAAYAHMFDLAARLAAGLEQTIARRGLPWCVTRIGARTEFQFAPAPPRNGTIAGLQL
DGELEHIVHLYLLNRGVLITPFHNMMLVCPQTSAEDVDRLVAAFDACLGELL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1786349, Length=372, Percent_Identity=31.4516129032258, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788044, Length=269, Percent_Identity=29.368029739777, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789759, Length=276, Percent_Identity=26.8115942028986, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789016, Length=320, Percent_Identity=25, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144271, Length=304, Percent_Identity=25.3289473684211, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=32.3943661971831, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=432, Percent_Identity=23.1481481481481, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 49018; Mature: 49018

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRHGINLARARALLARERDAFERAMPKSRARSAEAAQHLLFGVPLHWMRDWSTPFSLY
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
VDHARGAHFTDIDGHRYADFCLGDTGAMFGHAPEPVARALAEQAARGYTTMLPSEDAAWV
EECCCCCCCCCCCCCEEEHEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHH
AAELARRFRLPYWQFALSASDANRFVLRWARAATGRKHIVVFNGCYHGTVDDVFVDLADG
HHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHEEECCCC
RPVQRDSLLGQAHDLSAYTRVVEFNDLAALEAALKDGEVACVLAEPALTNIGMVLPDAGF
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCEECCCCCH
WREARALTRRHGTLLAIDETHTISSGPGGYALAHGLEPDLLVVGKPIGGGVPCAVYGFSA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEEEECCCH
SCAERAQHAKASAPPGHSGIGTTLTANMLAMRAIRATLADVMTDAAYAHMFDLAARLAAG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQTIARRGLPWCVTRIGARTEFQFAPAPPRNGTIAGLQLDGELEHIVHLYLLNRGVLIT
HHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEE
PFHNMMLVCPQTSAEDVDRLVAAFDACLGELL
ECCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIRHGINLARARALLARERDAFERAMPKSRARSAEAAQHLLFGVPLHWMRDWSTPFSLY
CCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
VDHARGAHFTDIDGHRYADFCLGDTGAMFGHAPEPVARALAEQAARGYTTMLPSEDAAWV
EECCCCCCCCCCCCCEEEHEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCHHHH
AAELARRFRLPYWQFALSASDANRFVLRWARAATGRKHIVVFNGCYHGTVDDVFVDLADG
HHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHEEECCCC
RPVQRDSLLGQAHDLSAYTRVVEFNDLAALEAALKDGEVACVLAEPALTNIGMVLPDAGF
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCCEECCCCCH
WREARALTRRHGTLLAIDETHTISSGPGGYALAHGLEPDLLVVGKPIGGGVPCAVYGFSA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCEEEECCCH
SCAERAQHAKASAPPGHSGIGTTLTANMLAMRAIRATLADVMTDAAYAHMFDLAARLAAG
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEQTIARRGLPWCVTRIGARTEFQFAPAPPRNGTIAGLQLDGELEHIVHLYLLNRGVLIT
HHHHHHHCCCCHHHHHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEE
PFHNMMLVCPQTSAEDVDRLVAAFDACLGELL
ECCCEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]