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The map label for this gene is plcB [H]

Identifier: 76817913

GI number: 76817913

Start: 477693

End: 479153

Strand: Direct

Name: plcB [H]

Synonym: BURPS1710b_A0331

Alternate gene names: 76817913

Gene position: 477693-479153 (Clockwise)

Preceding gene: 76819739

Following gene: 76819152

Centisome position: 15.01

GC content: 68.79

Gene sequence:

>1461_bases
ATGCCGCGCCGCCCGGGCGCCCGGACGGGCGACGCAGCCGCTCGCGCACCGCATGAAAAGGAGAACGCCGTGGCGAAGAT
CCTCGAAAACGTCGAGCACATCGTCGTCGTGATGTTCGAGAACCGCTCGTTCGACACGATGCTCGGCTGGCTCTATTCGC
CCGACGCGCCGCCCGCGCACGTGCTGCCGCCGCAGAGCTCGCCGCAATTCGACGGCCTGAGGCCCGAGATGTCGAATCCG
TCGAAGGCGGGCAAGACCATCGCCGTCACGCGCGAGGCGCTCAACAGCGTGATCCCCGATCCGCAGGAGACATTCGTCAA
CGTGACGAGCCAGATCCTCGGCCCGAGCGATGCAGGCAACCCGTGGCCGCCGATGCAGGGCTTCGTCGTCAATTACGAAA
CGACGCAGACGACCGATCCGGGCCACGTGATGCAATGCCACAGCGTCGCGCAACTGCCGACGCTCACGGCGCTCGCGCGC
GCGTACGCGGTGTCCGACGCGTGGTTCGCGTCGGTGCCGAGCCAGACCTGGCCGAACCGCGCGTTCGCGCACGCCGGCAC
GTCGAACGGGCACGTGAACAACGGCGCGATCCCCGATCCGCTCGAATGGGACGTGCCGACGATCTTCGACGTGCTGTCGT
CGATCGGCGCGAGCTGGGCGGTGTACAGCGACACGACCGTCACGCCGTCGCTCACGCACACGATGTTCCCGTCGCTGTGG
AAGGATGCATACGCCGAGCGCTTCAGGCGCTTCCCGGATTTCGTCGCCGCGTGCGGAAACGACGCGCTGCCGCAATACGC
GTTCATCGAGCCGAGCTTCCTGTTCGAGCCGAACGACCAGCATCCGCCGCACGACGTGCGCGCGGGCGACAAGTTCCTGC
ACGACATCTGGACGGCGATCCGCACGTCGCCCGGCTGGCCGCGCACGCTGCTCGTCGTGACCTATGACGAGCACGGCGGC
TGCTTCGACCACGTGCTGCCGCCCGCGAACGCGACCCCGCCGGACAACGCGAGCAACCCCGGCGATGAGGCGTTCGGCTT
CTATCGCTTCGGCGTGCGGGTGCCGGCCGTCGTCGTGTCGCCGTACATCGAGGCGGGCACCGTGTTCCGCTCGCCGACGA
ACGTCCCGTACGATCACACGTCGATCCTCGCGACGCTGCGCGACTGGCTCGATATTCCGCCCGACCGGATGCTCGGCAGC
GCGCGGATCGCGAACGCGCCGACGCTCGCGCAACTCGTCACACGCGACACGCCGCGCACCGATCTGCCGACGATCGCCGC
GCCGTCGACCGACTTCGTCCATCCGCCGCCGGATGCGCCGCTCAACGACGTGCAAAAGAGCCTCGTGACGGGCACGTCGC
GGCGCGACGGGCACGATCCGGCCGCCGGCGTCGCACCGATCGCGACGCGCCAGCACGCGCTCGATTTCTTCAAGCGCCGG
GCGGCGGCAAAAACGGCTTAG

Upstream 100 bases:

>100_bases
GCTGCGGCGCGGGCGCCCGTAGGCGCGCGCCGCCTCGTCTTGTTTTTCCCGAAATATCGGATATACCTAATCCATCCGAT
TGCGTGAGGACGCGGGGGGC

Downstream 100 bases:

>100_bases
CGGCTTGATCGGGCAACGTGGCGGCTGAGCGGTGCAGCGACTCGACGATGGGTCGACTTGCCGCCCCGACGACCCGACGA
CCCGACGACCTGGCGACCTG

Product: phosphoesterase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MPRRPGARTGDAAARAPHEKENAVAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNP
SKAGKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALAR
AYAVSDAWFASVPSQTWPNRAFAHAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLW
KDAYAERFRRFPDFVAACGNDALPQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGG
CFDHVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGS
ARIANAPTLAQLVTRDTPRTDLPTIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRR
AAAKTA

Sequences:

>Translated_486_residues
MPRRPGARTGDAAARAPHEKENAVAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNP
SKAGKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALAR
AYAVSDAWFASVPSQTWPNRAFAHAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLW
KDAYAERFRRFPDFVAACGNDALPQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGG
CFDHVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGS
ARIANAPTLAQLVTRDTPRTDLPTIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRR
AAAKTA
>Mature_485_residues
PRRPGARTGDAAARAPHEKENAVAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPS
KAGKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARA
YAVSDAWFASVPSQTWPNRAFAHAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWK
DAYAERFRRFPDFVAACGNDALPQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGC
FDHVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSA
RIANAPTLAQLVTRDTPRTDLPTIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRA
AAKTA

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 52949; Mature: 52817

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRPGARTGDAAARAPHEKENAVAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAH
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCC
VLPPQSSPQFDGLRPEMSNPSKAGKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGN
CCCCCCCCCCCCCCCCCCCCHHCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
PWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
AFAHAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLW
CEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
KDAYAERFRRFPDFVAACGNDALPQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAI
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
RTSPGWPRTLLVVTYDEHGGCFDHVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVS
HCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCC
PYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRT
CCHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHCCCCHHHHHHHCCCCCC
DLPTIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AAAKTA
HHHCCC
>Mature Secondary Structure 
PRRPGARTGDAAARAPHEKENAVAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAH
CCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCC
VLPPQSSPQFDGLRPEMSNPSKAGKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGN
CCCCCCCCCCCCCCCCCCCCHHCCCEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
PWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNR
CCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
AFAHAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLW
CEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
KDAYAERFRRFPDFVAACGNDALPQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAI
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
RTSPGWPRTLLVVTYDEHGGCFDHVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVS
HCCCCCCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCC
PYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRT
CCHHCCCEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHCCCCHHHHHHHCCCCCC
DLPTIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AAAKTA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8757862; 9634230; 12218036 [H]