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The map label for this gene is ptrB [C]

Identifier: 76817831

GI number: 76817831

Start: 516368

End: 518716

Strand: Direct

Name: ptrB [C]

Synonym: BURPS1710b_A0367

Alternate gene names: 76817831

Gene position: 516368-518716 (Clockwise)

Preceding gene: 76817677

Following gene: 76818139

Centisome position: 16.23

GC content: 68.45

Gene sequence:

>2349_bases
ATGACGCGGCGCGCGTGGGTGATAGTCGGAATCGCGCATGGATACGGGCGAAGCGGAAAACGGCACGGCGTCGGCACCGG
CTTCGGACAGCCCCGACACGACGAAGGCACGCTTGCGACGTGTACGCGGCGCGCCACGCGCGCGCCGCGAATGCCTGCGG
CGGACGCGTCGCAATCGACGAGCCGGCAGCCAAGCGCGAGCGCCGCTCGTCTTACGCTACCATTACACGCCATGCCCCTT
GCAAAGAACGCCATGCCTCATGCTTCCTGGCCCGAACAGGCCGATCCGCATCAATTCCTCGAAGAACTGGACAGCGCCGC
GAGCGTCGGCTGGGTCGACGCGCAAAACGCCCGCACGCACGATGCGCCCTGGCTCGACGAAGCGCACTATCGCGCGCTGG
TCGAGCGCTTCACCCGGGCGCTGCTGCCGCGCGAGCGCCCGGTGATTCCGCAGCGCTGGCAGGACTGGGCGTACGACGTC
TGGCAGGACGAACAGCATCCGAAGGGCCTGTGGCGGCGCACGCGATGGACGAGCTGGCGCAGCGGCCACGCGGACTGGCA
GACGTTGATCGACCTCGATGCGCTTGGTGAAGCGCAAGGCGTGCAGTGGGTGTTCGACGATCAGCTCATCCTCGAGCCGG
ACGGCGATCGTGCGCTGATCGTGCTGTCCGACGGCGGCGCCGACGCGGTCGTCGTCCGCGAGTTCGACATCGCGCAATGC
CGGTTCGTCGACGACGGCTTCTCGATCGAAGCGGCCGGCAAGCATTCGGTCGAATGGATCGATCGCGACACGATCTACGT
CGGCTGGGACGACGGCGGCGCCACCGTCACGCGCTCCGGCTATCCGCGCGAAGTGCGGCGCTGGACGCGCGGCACGCCGC
TGTCCAGCGCGCCCGTGGTGTTTCGCGGCGCGCGCGGCGACATCTCGGTCGATGCGCAATACGATCCGCTCGACCGGCAT
CACGCGATCGAGCAGGCGATCAATTTCTACGACGCGAACACGTATCGCCTCGCCGAGGACGGCGCGTGGGCGCGCTACGA
CGTGCCACCGCACGTCGAAGTCGGTTACTGGAGCGGGTGGCTGCTGCTCCAGCCGCGGCTCGACTGGACTTGCGGCGGCG
CGCGCTACGCGGGCGGCAGCCTGCTCGCGATCCGCGAGGACGCGTTCGTCGCCGGTGAGCGCGCGTTCGCCGCGCTGTTC
GAGCCGAACGAGCGCACGTCCGCATGCGGCTGGACGCACACGCGCCGCTACGTGCTGGTGTCGTGGCTCGACGACGTGCT
CACGCGCACGATGCTCTGGCTTCCCGAACGTCAGGATGACGGAGCATGGCGCTGGCATGCTCGTCCGTTCCCCGCGCGAG
GGCTCGCGCAAGTGGACGTGTCGCCCGTCGAGCCCACGTTCGACGACGAGGTGTACGTGAGCGTCGACGATTACCTGAAG
CCGCCCGAGTATTCGCTCGCGAATCTCGCCAGCGACGACCTGTCCGCCTGGACGCTGCTCGACCGCTGGCCGACGCAGTT
CGACGCGTCCGAACTGACGGTGCGGCGCGAACACGCGCGCTCGCGCGACGGCACGCTCGTGCCTTATACGCTGGTCGGGC
CGCGCGACGTGCTGGACAATGCGGCGCGCGCGCCGCGCCCCTGCCTGTTGAACGGCTACGGCGGCTTCGCGATTGCGCTC
ACGCCCGATTACGATCCGTTGCTCGGCATCGGCTGGCTCGAGAAAGGCGGCATCGCGGTGTTCGCCCATATTCGCGGCGG
CGGCGAGTTCGGCACGCAGTGGCACGAATCGGCGCGGCAAACGCAACGGCAGCGATCGTTCGACGATTTCATCGCGGTCG
CCGAAAAACTCGTCGCGGACGGCGTGACGAGCGCCGCGCAACTGGGTATTCGCGGCGGCAGCAACGGCGGGCTGCTGGTC
GCGGCATGCATGATTCAGCGCCCGGACCTGTTCGGCGCGGTGGTGAGCGACGTGCCGCTTCTCGACATGCAGCGCTATGC
GCTGCTGCACGCGGGCGCATCGTGGCTGGACGAATTCGGCGATCCCGACGATCCGGCGCATGCGTCGGCGCTCGCGGCCT
ACTCGCCGTATCACCGGGTCGCGCGCGACATCGCGTATCCGCCCGCGCTGTTCACGACATCGACGAGCGACGACCGCGTG
CATCCCGCCCATGCGAGAAAAATGGTCGCGCGCATGCAGGCGCAAGGGCACCGAAACGTATGGCTGATCGAGAAAACCGA
TGGCGGCCACGGCAGCGCGGACGCGATCGATACCGCCGAGCACGAAGCGATCGGCTATGTGTTTCTGTGGACTCACTTGT
CCCGCGGCGCGCATGACGCGCGCGAGTGA

Upstream 100 bases:

>100_bases
GACATGGGCCAGGCTGGACGGCGAATGCCGTCTATAGCCATCGGGAGTTCACTATGAAAGAGCCGCCGCAAAAGCCGAAC
CAATTATTTTTCATTTCGAA

Downstream 100 bases:

>100_bases
ATCGCCGTGACGGTGCGTGCGGTCGCTCGCCCCGTCTTTCCTGCATGCGGGGCGGATCGACGTTGCCGCCACGTCGGATC
GCAGCAGGCGCGGCGGCGCG

Product: prolyl oligopeptidase family protein

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 782; Mature: 781

Protein sequence:

>782_residues
MTRRAWVIVGIAHGYGRSGKRHGVGTGFGQPRHDEGTLATCTRRATRAPRMPAADASQSTSRQPSASAARLTLPLHAMPL
AKNAMPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTRALLPRERPVIPQRWQDWAYDV
WQDEQHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQC
RFVDDGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRH
HAIEQAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALF
EPNERTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLK
PPEYSLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIAL
TPDYDPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLV
AACMIQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRV
HPAHARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE

Sequences:

>Translated_782_residues
MTRRAWVIVGIAHGYGRSGKRHGVGTGFGQPRHDEGTLATCTRRATRAPRMPAADASQSTSRQPSASAARLTLPLHAMPL
AKNAMPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTRALLPRERPVIPQRWQDWAYDV
WQDEQHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQC
RFVDDGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRH
HAIEQAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALF
EPNERTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLK
PPEYSLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIAL
TPDYDPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLV
AACMIQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRV
HPAHARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE
>Mature_781_residues
TRRAWVIVGIAHGYGRSGKRHGVGTGFGQPRHDEGTLATCTRRATRAPRMPAADASQSTSRQPSASAARLTLPLHAMPLA
KNAMPHASWPEQADPHQFLEELDSAASVGWVDAQNARTHDAPWLDEAHYRALVERFTRALLPRERPVIPQRWQDWAYDVW
QDEQHPKGLWRRTRWTSWRSGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQCR
FVDDGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVVFRGARGDISVDAQYDPLDRHH
AIEQAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGWLLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFE
PNERTSACGWTHTRRYVLVSWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLKP
PEYSLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDNAARAPRPCLLNGYGGFAIALT
PDYDPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQTQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVA
ACMIQRPDLFGAVVSDVPLLDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRVH
PAHARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDARE

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=297, Percent_Identity=37.7104377104377, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI108860686, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI284172420, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI284172413, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI70778815, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI284172438, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI284172431, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI108860692, Length=286, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=7e-17,
Organism=Escherichia coli, GI1788150, Length=263, Percent_Identity=31.1787072243346, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24583414, Length=300, Percent_Identity=36.3333333333333, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI221510989, Length=233, Percent_Identity=39.9141630901288, Blast_Score=180, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 87069; Mature: 86937

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRAWVIVGIAHGYGRSGKRHGVGTGFGQPRHDEGTLATCTRRATRAPRMPAADASQST
CCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
SRQPSASAARLTLPLHAMPLAKNAMPHASWPEQADPHQFLEELDSAASVGWVDAQNARTH
CCCCCCCCEEEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCC
DAPWLDEAHYRALVERFTRALLPRERPVIPQRWQDWAYDVWQDEQHPKGLWRRTRWTSWR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
SGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQC
CCCCCHHHHHHHHHCCCCCCCEEEECCEEEECCCCCEEEEEEECCCCCEEEEEECCCEEE
RFVDDGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVV
EEECCCCEEEECCCCCEEEECCCEEEEEECCCCCEEECCCCCHHHHHHHCCCCCCCCCEE
FRGARGDISVDAQYDPLDRHHAIEQAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGW
EECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEEEECCE
LLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFEPNERTSACGWTHTRRYVLV
EEECCCCCEEECCCEECCCEEEEEECCCEECCCHHEEEEECCCCCCCCCCCCCCCCEEHH
SWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLK
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCC
PPEYSLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDN
CCCCHHHHHCCCCCHHEEHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
AARAPRPCLLNGYGGFAIALTPDYDPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQ
HHCCCCCEEEECCCCEEEEECCCCCCCEECCEECCCCEEEEEEEECCCCCCHHHHHHHHH
TQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVAACMIQRPDLFGAVVSDVPL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHCCCC
LDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRV
HHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCC
HPAHARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCC
RE
CC
>Mature Secondary Structure 
TRRAWVIVGIAHGYGRSGKRHGVGTGFGQPRHDEGTLATCTRRATRAPRMPAADASQST
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
SRQPSASAARLTLPLHAMPLAKNAMPHASWPEQADPHQFLEELDSAASVGWVDAQNARTH
CCCCCCCCEEEEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCC
DAPWLDEAHYRALVERFTRALLPRERPVIPQRWQDWAYDVWQDEQHPKGLWRRTRWTSWR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
SGHADWQTLIDLDALGEAQGVQWVFDDQLILEPDGDRALIVLSDGGADAVVVREFDIAQC
CCCCCHHHHHHHHHCCCCCCCEEEECCEEEECCCCCEEEEEEECCCCCEEEEEECCCEEE
RFVDDGFSIEAAGKHSVEWIDRDTIYVGWDDGGATVTRSGYPREVRRWTRGTPLSSAPVV
EEECCCCEEEECCCCCEEEECCCEEEEEECCCCCEEECCCCCHHHHHHHCCCCCCCCCEE
FRGARGDISVDAQYDPLDRHHAIEQAINFYDANTYRLAEDGAWARYDVPPHVEVGYWSGW
EECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCEEEEEECCE
LLLQPRLDWTCGGARYAGGSLLAIREDAFVAGERAFAALFEPNERTSACGWTHTRRYVLV
EEECCCCCEEECCCEECCCEEEEEECCCEECCCHHEEEEECCCCCCCCCCCCCCCCEEHH
SWLDDVLTRTMLWLPERQDDGAWRWHARPFPARGLAQVDVSPVEPTFDDEVYVSVDDYLK
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEECCCCCCCCCCCEEEEEHHHCCC
PPEYSLANLASDDLSAWTLLDRWPTQFDASELTVRREHARSRDGTLVPYTLVGPRDVLDN
CCCCHHHHHCCCCCHHEEHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHH
AARAPRPCLLNGYGGFAIALTPDYDPLLGIGWLEKGGIAVFAHIRGGGEFGTQWHESARQ
HHCCCCCEEEECCCCEEEEECCCCCCCEECCEECCCCEEEEEEEECCCCCCHHHHHHHHH
TQRQRSFDDFIAVAEKLVADGVTSAAQLGIRGGSNGGLLVAACMIQRPDLFGAVVSDVPL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEEHHCCCCHHHHHHHCCCC
LDMQRYALLHAGASWLDEFGDPDDPAHASALAAYSPYHRVARDIAYPPALFTTSTSDDRV
HHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCCCC
HPAHARKMVARMQAQGHRNVWLIEKTDGGHGSADAIDTAEHEAIGYVFLWTHLSRGAHDA
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCC
RE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]