Click here to switch to the map view.

The map label for this gene is mdlB [H]

Identifier: 76817657

GI number: 76817657

Start: 796378

End: 797703

Strand: Direct

Name: mdlB [H]

Synonym: BURPS1710b_A0604

Alternate gene names: 76817657

Gene position: 796378-797703 (Clockwise)

Preceding gene: 76818830

Following gene: 76818857

Centisome position: 25.03

GC content: 69.23

Gene sequence:

>1326_bases
ATGATTCTTCGCTTTTATCGAACGATTTCGTTTGAAGATTATCGCTTTTTATCGATGTGGCGGCTGCATAGAATGGCTGC
GTCGCGTCAGGAAAGCCCAACAATGAGCACTGTCACCTCTTCTCCCATCGTCGAAACGAGCCGGCCCGCGGCCAGCGCCG
CCGCCACGCCGCGCGCGCTGCGGGGCGTCCTGAGCCTGCACGACTTCGAAGCGAAGGCGCGCCGCGCGCTGCCGCGCCCG
ATCTTCGGCTACGTCGGCGGCGCGGCCGAAGACAACCGCACCCGCGACGACAACCGAGCCGCCTTCGACGAATACGGCTT
CGTCACGCGCGTGCTGCGCGACGTCTCGCAGCGGCAGCAGGGTGTCGAGCTGTTCGGCCGGCGCTACGCGTCGCCGTTCG
GCATCGCGCCGATGGGCATCAACGCGCTGTCGACGTATCGCGGCGACATCGTGCTCGCGCGCGCCGCGCAGCACGCGGGG
ATCGCGTCGATCATGAGCGGCTCGTCGCTGATTCCGCTCGAAGACGTGGCCGCCGCCGCGCCCGACACGTGGTTCCAGGC
CTACCTGCCGGGCGATGCCGGGCGCATCCGCGCGCTCGTCGAGCGCGTCGCGCGCGCCGGCTACCGGACGCTCGTCGTCA
CCGTCGACATTCCGGTGTCGGCGAACCGCGAAAACAACGTGCGCAGCGGCTTCTCCACGCCGCTGCGCCCGAGCCCGCGC
CTGTTCTGGGACGGGCTGACGCGGCCGCGCTGGCTGTTGGGCACGTTCGCGCGCACGTTGCTCGCGCACGGCATGCCGCA
TTTCGAGAATTCGTTCGCGACGCGCGGCGCGCCGATTCTTTCCGCCAACGTGCTGCGCGACTTTTCCGCGCGCGACCATC
TGAGCTGGGCGCACGTGCGGCAAATCCGCGAGCAATGGGCGGGCGAGCTCGTGATCAAGGGCGTGCTCAGCGTCGAGGAC
GCGCGGATCGCGCGCGAGGCGGGCGCCGACGGCATCATCCTGTCCAACCACGGCGGCCGCCAGCTGGACGGCGCCGTATC
GCCGATGCGGATCCTGCGCGACGTGGTGCAGGCTGTCGGCAACGGGTATCCGGTGATGATCGACAGCGGTTTTCGCCGGG
GCTCGGACGTGCTGAAGGCCGTGGCGCTCGGCGCGCGGATGGTGTTCGTCGGACGGCCGTTCAACTATGCGGCCGCGGTG
GCGGGCGAGGCCGGCGTCGCGCATGCGATCCGCCTGCTGCGCGAAGAGGTGGACCGGAATCTGGCGATGCTGGGCGCGAA
CGGCTGCGACGCGCTGACGCCCGACATGCTGATTCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
GGACGAGATCGACAGATGAAGCCGCTGCGCGGCGGTCGAGAAATTCGCGCAGGTCGCCGCCCAATAAAAGGCATCGAGTT
GCTTGAGCGTCGGAGTAGCC

Downstream 100 bases:

>100_bases
CGACGCCGGGCGGTTCGATCGCGAAGGGCCGCCGCGGCGGCGGTCATGCGCGCCCGACGACGCCTCGCCGCCGAGGGTCG
AGGCGTCGTCGGGCGCTTGC

Product: putative L(+)-mandelate dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MILRFYRTISFEDYRFLSMWRLHRMAASRQESPTMSTVTSSPIVETSRPAASAAATPRALRGVLSLHDFEAKARRALPRP
IFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAG
IASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPLRPSPR
LFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKGVLSVED
ARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAV
AGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR

Sequences:

>Translated_441_residues
MILRFYRTISFEDYRFLSMWRLHRMAASRQESPTMSTVTSSPIVETSRPAASAAATPRALRGVLSLHDFEAKARRALPRP
IFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAG
IASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPLRPSPR
LFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKGVLSVED
ARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAV
AGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR
>Mature_441_residues
MILRFYRTISFEDYRFLSMWRLHRMAASRQESPTMSTVTSSPIVETSRPAASAAATPRALRGVLSLHDFEAKARRALPRP
IFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQGVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAG
IASIMSGSSLIPLEDVAAAAPDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPLRPSPR
LFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVRQIREQWAGELVIKGVLSVED
ARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVGNGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAV
AGEAGVAHAIRLLREEVDRNLAMLGANGCDALTPDMLIRKR

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=366, Percent_Identity=34.4262295081967, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI7705393, Length=366, Percent_Identity=34.4262295081967, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI11068137, Length=380, Percent_Identity=31.5789473684211, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1790033, Length=380, Percent_Identity=36.8421052631579, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI193208036, Length=386, Percent_Identity=32.9015544041451, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32566217, Length=308, Percent_Identity=29.5454545454545, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323587, Length=390, Percent_Identity=29.2307692307692, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI78707188, Length=380, Percent_Identity=33.9473684210526, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI281363140, Length=380, Percent_Identity=33.9473684210526, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78707190, Length=380, Percent_Identity=33.9473684210526, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 48335; Mature: 48335

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILRFYRTISFEDYRFLSMWRLHRMAASRQESPTMSTVTSSPIVETSRPAASAAATPRAL
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHCCHHHH
RGVLSLHDFEAKARRALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQ
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAGIASIMSGSSLIPLEDVAAAA
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCHHHHHCCCCCCCHHHHHHCC
PDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPLRPSPR
CCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVR
HHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCHHCCCCCHHHHHHHHHCCHHCCCHHHHHH
QIREQWAGELVIKGVLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
NGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAVAGEAGVAHAIRLLREEVDRN
CCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCC
LAMLGANGCDALTPDMLIRKR
EEEECCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MILRFYRTISFEDYRFLSMWRLHRMAASRQESPTMSTVTSSPIVETSRPAASAAATPRAL
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHCCHHHH
RGVLSLHDFEAKARRALPRPIFGYVGGAAEDNRTRDDNRAAFDEYGFVTRVLRDVSQRQQ
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GVELFGRRYASPFGIAPMGINALSTYRGDIVLARAAQHAGIASIMSGSSLIPLEDVAAAA
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEHHHHCCHHHHHCCCCCCCHHHHHHCC
PDTWFQAYLPGDAGRIRALVERVARAGYRTLVVTVDIPVSANRENNVRSGFSTPLRPSPR
CCHHHEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LFWDGLTRPRWLLGTFARTLLAHGMPHFENSFATRGAPILSANVLRDFSARDHLSWAHVR
HHCCCCCCCHHHHHHHHHHHHHCCCCCHHCCHHCCCCCHHHHHHHHHCCHHCCCHHHHHH
QIREQWAGELVIKGVLSVEDARIAREAGADGIILSNHGGRQLDGAVSPMRILRDVVQAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHC
NGYPVMIDSGFRRGSDVLKAVALGARMVFVGRPFNYAAAVAGEAGVAHAIRLLREEVDRN
CCCEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCC
LAMLGANGCDALTPDMLIRKR
EEEECCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]