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The map label for this gene is lcfB [H]

Identifier: 76817551

GI number: 76817551

Start: 807410

End: 809236

Strand: Direct

Name: lcfB [H]

Synonym: BURPS1710b_A0616

Alternate gene names: 76817551

Gene position: 807410-809236 (Clockwise)

Preceding gene: 76818003

Following gene: 76818021

Centisome position: 25.38

GC content: 69.29

Gene sequence:

>1827_bases
ATGTCGAATGTGCTAGCGCGGCGCGCGGCCGCATCCGTCAATGTTCGGCACCACCCCGTTCGGCCATTTACATCGATGCG
CCGCGACCGCGCGCCGTGTCCATTCGCTACTCTTTCGGCAGCCAGGATCGGCCGCGTTCGCGCGACGGTCGTTAGCCATC
GCGAGCTTCGCCGCATCGCTCGACGTGCGCCGCCCGAGTTTCAAAACGGAGGTGGAATCATGCGTAATTTCTTCGATCTG
GTCGAACAGGCGGCATGCCGCACGCCCGATGCCGAGGCGCTCGTCGCCGGCGACGCTCGGCTCACCTATCGCGCACTCGC
GGCGCTCGGCCTGGCGTTCGCCGAGCGGCTGCAAGCGCTCGAGATCGCCCAGGGCGAGCGGCTCGCGATCTTTCTCGACA
AGCGCATCGAGACGGTCGTCGCCATGCTCGGCGCCGCGGCGGCCGGCGTCGTGTTCGTGCCGATCAACCCGGTCCTCAAG
CCCGAACAGGTTCATCACATCCTCGTCGACAGCGGCGCGCGCTGTCTCGTCACGTCGTCGCTGCGCGCGCGCATACTCGG
CGACGGCGTGCTCGCGGGCATGCCGTACGTGATCCTGACCGATGCGAAGACGCTTGCACCGGCGCCGGCTTCGCGCACCA
CGTTTCTGCGATGGATGCAGCCCGATGCGCCGCCCGAGTCCGTCGCGAACCGGCACGCGAGCGCCGCGCCCGAGCCGCGA
GCGGACACGATCGACGCCGATCTCGCCGCACTCCTTTACACGTCCGGCTCGACGGGCCGCCCCAAGGGCGTGATGCTCAG
CCACCGCAATCTGCTCGAGGGCGCGTGGAGCGTCGCGCAATACCTGCGCCATACCGCGCAGGATCGCATTCTCGCCGCGC
TGCCGCTCAGCTTCGACGCCGGCTTGAGCCAGTTGACGACCGCATGGGCCGCGGGCGCGAGCGCCGTGCTCGTCAACTAC
CTGATGCCCGCGGACGTCGTCGAGATCTGCGTGCGCGAACGCATCACCGGCTTCGCGGGCGTACCGCCGCTCTGGATTCA
GCTCGCGCGCGCGGCGTGGCCCGGCGAAGCGCGCGCGCGGCTGCGCTATTTCGCGAACACCGGCGGCCATCTGCCGCGGC
CGGTGCTGCACGCGCTGCGCGAACTGTTTCCGAGCGCGTCGCCGTATCTGATGTACGGGCTGACCGAAGCGTTCCGCTCG
ACCTACCTCGATCCCGCCGAAGTCGACCGTCGCCCCGATTCGATCGGCAAGGCGGTGCCGAACGCCAGAATTCTCGTCGT
GCGCGAGGACGGCGCGCCGTGCGCGCCGAACGAGGTCGGCGAGCTGGTCCACGTCGGCGCGTGCGTGACGCTCGGCTACT
GGAACGATCCGGCGCGCACCGCGCTGCGCTACCGGCCTTCGCCCGAGGCGAAGCCGGGCGGCGCGCCACGTGAGACGGCC
GTCTGGTCCGGTGATCTCGTGCGCCGCGACGACGACGGATTCCTCTATTTCGTCGCCCGCAACGATGCGCAGATCAAGAG
TTCCGGCTACCGGATCAGCCCGGAAGAGATCGAGGAGGTCGCGCACGCGAGCGGGCTCGTGGCCGAAGCGGTCGCGCTCG
GCGTGCCGCACGACGAACTCGGCGAATCGATCACGCTCGTCGTCGTACCGCTCGACGCCGACACGTTCAGGCCCGACGCG
CTGCGCGCGCGATGCGCACAGCAACTTCCCCCGTACATGGTGCCGCATACGATCGCCACGCGCACGTCGCTGCCGCGCAA
TCCGAACGGGAAATTCGATCGCGTCGCGCTGCGCGCCGACGCGGCGAACCTCGTCGAAACGCTCTGA

Upstream 100 bases:

>100_bases
AACCGAATTGAAAGACCGTGTCCGAGCGCACCGGCGAAATTCATCAGGACAATTGACGATCACGTCGAGCAGACGATCTC
CTCCATGCGCGCGCGTGTGC

Downstream 100 bases:

>100_bases
AGACGCGCCGGATCGCCGCGCGAGACGCTCGTCTACGCGCGACGTCCGCCGTCCGCCTCCGTTGCCTACGCCGTACCCGG
CGCCCCGGGCGGCGCGACGT

Product: AMP-binding domain-containing protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MSNVLARRAAASVNVRHHPVRPFTSMRRDRAPCPFATLSAARIGRVRATVVSHRELRRIARRAPPEFQNGGGIMRNFFDL
VEQAACRTPDAEALVAGDARLTYRALAALGLAFAERLQALEIAQGERLAIFLDKRIETVVAMLGAAAAGVVFVPINPVLK
PEQVHHILVDSGARCLVTSSLRARILGDGVLAGMPYVILTDAKTLAPAPASRTTFLRWMQPDAPPESVANRHASAAPEPR
ADTIDADLAALLYTSGSTGRPKGVMLSHRNLLEGAWSVAQYLRHTAQDRILAALPLSFDAGLSQLTTAWAAGASAVLVNY
LMPADVVEICVRERITGFAGVPPLWIQLARAAWPGEARARLRYFANTGGHLPRPVLHALRELFPSASPYLMYGLTEAFRS
TYLDPAEVDRRPDSIGKAVPNARILVVREDGAPCAPNEVGELVHVGACVTLGYWNDPARTALRYRPSPEAKPGGAPRETA
VWSGDLVRRDDDGFLYFVARNDAQIKSSGYRISPEEIEEVAHASGLVAEAVALGVPHDELGESITLVVVPLDADTFRPDA
LRARCAQQLPPYMVPHTIATRTSLPRNPNGKFDRVALRADAANLVETL

Sequences:

>Translated_608_residues
MSNVLARRAAASVNVRHHPVRPFTSMRRDRAPCPFATLSAARIGRVRATVVSHRELRRIARRAPPEFQNGGGIMRNFFDL
VEQAACRTPDAEALVAGDARLTYRALAALGLAFAERLQALEIAQGERLAIFLDKRIETVVAMLGAAAAGVVFVPINPVLK
PEQVHHILVDSGARCLVTSSLRARILGDGVLAGMPYVILTDAKTLAPAPASRTTFLRWMQPDAPPESVANRHASAAPEPR
ADTIDADLAALLYTSGSTGRPKGVMLSHRNLLEGAWSVAQYLRHTAQDRILAALPLSFDAGLSQLTTAWAAGASAVLVNY
LMPADVVEICVRERITGFAGVPPLWIQLARAAWPGEARARLRYFANTGGHLPRPVLHALRELFPSASPYLMYGLTEAFRS
TYLDPAEVDRRPDSIGKAVPNARILVVREDGAPCAPNEVGELVHVGACVTLGYWNDPARTALRYRPSPEAKPGGAPRETA
VWSGDLVRRDDDGFLYFVARNDAQIKSSGYRISPEEIEEVAHASGLVAEAVALGVPHDELGESITLVVVPLDADTFRPDA
LRARCAQQLPPYMVPHTIATRTSLPRNPNGKFDRVALRADAANLVETL
>Mature_607_residues
SNVLARRAAASVNVRHHPVRPFTSMRRDRAPCPFATLSAARIGRVRATVVSHRELRRIARRAPPEFQNGGGIMRNFFDLV
EQAACRTPDAEALVAGDARLTYRALAALGLAFAERLQALEIAQGERLAIFLDKRIETVVAMLGAAAAGVVFVPINPVLKP
EQVHHILVDSGARCLVTSSLRARILGDGVLAGMPYVILTDAKTLAPAPASRTTFLRWMQPDAPPESVANRHASAAPEPRA
DTIDADLAALLYTSGSTGRPKGVMLSHRNLLEGAWSVAQYLRHTAQDRILAALPLSFDAGLSQLTTAWAAGASAVLVNYL
MPADVVEICVRERITGFAGVPPLWIQLARAAWPGEARARLRYFANTGGHLPRPVLHALRELFPSASPYLMYGLTEAFRST
YLDPAEVDRRPDSIGKAVPNARILVVREDGAPCAPNEVGELVHVGACVTLGYWNDPARTALRYRPSPEAKPGGAPRETAV
WSGDLVRRDDDGFLYFVARNDAQIKSSGYRISPEEIEEVAHASGLVAEAVALGVPHDELGESITLVVVPLDADTFRPDAL
RARCAQQLPPYMVPHTIATRTSLPRNPNGKFDRVALRADAANLVETL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=543, Percent_Identity=25.5985267034991, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761345, Length=552, Percent_Identity=25.3623188405797, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI187761343, Length=552, Percent_Identity=25.3623188405797, Blast_Score=113, Evalue=7e-25,
Organism=Homo sapiens, GI115511026, Length=561, Percent_Identity=23.7076648841355, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI38505220, Length=571, Percent_Identity=24.6935201401051, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI42544132, Length=521, Percent_Identity=23.6084452975048, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=23.3590733590734, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI40807357, Length=550, Percent_Identity=25.2727272727273, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI58082049, Length=583, Percent_Identity=22.6415094339623, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI38524616, Length=543, Percent_Identity=25.9668508287293, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI8923896, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI253970489, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI195947378, Length=402, Percent_Identity=23.3830845771144, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=558, Percent_Identity=30.1075268817204, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI145693145, Length=563, Percent_Identity=26.6429840142096, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI221142682, Length=499, Percent_Identity=26.8537074148297, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1786801, Length=541, Percent_Identity=25.8780036968577, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=25.092936802974, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789201, Length=511, Percent_Identity=24.4618395303327, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786810, Length=384, Percent_Identity=25, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=544, Percent_Identity=24.8161764705882, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=544, Percent_Identity=23.1617647058824, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=561, Percent_Identity=24.777183600713, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=365, Percent_Identity=24.9315068493151, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=385, Percent_Identity=23.6363636363636, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=386, Percent_Identity=24.3523316062176, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=392, Percent_Identity=22.4489795918367, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=514, Percent_Identity=23.3463035019455, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=25.2354048964218, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581924, Length=567, Percent_Identity=24.1622574955908, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356441, Length=365, Percent_Identity=26.3013698630137, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648676, Length=501, Percent_Identity=25.1497005988024, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648260, Length=502, Percent_Identity=23.3067729083665, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355181, Length=491, Percent_Identity=23.4215885947047, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24656500, Length=363, Percent_Identity=26.1707988980716, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356947, Length=531, Percent_Identity=21.8455743879473, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI161076582, Length=367, Percent_Identity=26.158038147139, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=358, Percent_Identity=26.536312849162, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648257, Length=360, Percent_Identity=26.1111111111111, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358303, Length=295, Percent_Identity=24.406779661017, Blast_Score=76, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 65799; Mature: 65668

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVLARRAAASVNVRHHPVRPFTSMRRDRAPCPFATLSAARIGRVRATVVSHRELRRIA
CCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAPPEFQNGGGIMRNFFDLVEQAACRTPDAEALVAGDARLTYRALAALGLAFAERLQAL
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
EIAQGERLAIFLDKRIETVVAMLGAAAAGVVFVPINPVLKPEQVHHILVDSGARCLVTSS
HHCCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHEEEECCCCEEEEEHH
LRARILGDGVLAGMPYVILTDAKTLAPAPASRTTFLRWMQPDAPPESVANRHASAAPEPR
HHHHHHCCHHHHCCCEEEEECCHHHCCCCCCCHHEEEECCCCCCHHHHHHCCCCCCCCCC
ADTIDADLAALLYTSGSTGRPKGVMLSHRNLLEGAWSVAQYLRHTAQDRILAALPLSFDA
CCCCHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHC
GLSQLTTAWAAGASAVLVNYLMPADVVEICVRERITGFAGVPPLWIQLARAAWPGEARAR
CHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LRYFANTGGHLPRPVLHALRELFPSASPYLMYGLTEAFRSTYLDPAEVDRRPDSIGKAVP
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCC
NARILVVREDGAPCAPNEVGELVHVGACVTLGYWNDPARTALRYRPSPEAKPGGAPRETA
CCEEEEEECCCCCCCCHHHHHHEEECEEEEEECCCCHHHHHEEECCCCCCCCCCCCCCCE
VWSGDLVRRDDDGFLYFVARNDAQIKSSGYRISPEEIEEVAHASGLVAEAVALGVPHDEL
EECCCCEEECCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHH
GESITLVVVPLDADTFRPDALRARCAQQLPPYMVPHTIATRTSLPRNPNGKFDRVALRAD
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEHH
AANLVETL
HHHHHHCC
>Mature Secondary Structure 
SNVLARRAAASVNVRHHPVRPFTSMRRDRAPCPFATLSAARIGRVRATVVSHRELRRIA
CCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAPPEFQNGGGIMRNFFDLVEQAACRTPDAEALVAGDARLTYRALAALGLAFAERLQAL
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
EIAQGERLAIFLDKRIETVVAMLGAAAAGVVFVPINPVLKPEQVHHILVDSGARCLVTSS
HHCCCCEEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHEEEECCCCEEEEEHH
LRARILGDGVLAGMPYVILTDAKTLAPAPASRTTFLRWMQPDAPPESVANRHASAAPEPR
HHHHHHCCHHHHCCCEEEEECCHHHCCCCCCCHHEEEECCCCCCHHHHHHCCCCCCCCCC
ADTIDADLAALLYTSGSTGRPKGVMLSHRNLLEGAWSVAQYLRHTAQDRILAALPLSFDA
CCCCHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHC
GLSQLTTAWAAGASAVLVNYLMPADVVEICVRERITGFAGVPPLWIQLARAAWPGEARAR
CHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LRYFANTGGHLPRPVLHALRELFPSASPYLMYGLTEAFRSTYLDPAEVDRRPDSIGKAVP
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCC
NARILVVREDGAPCAPNEVGELVHVGACVTLGYWNDPARTALRYRPSPEAKPGGAPRETA
CCEEEEEECCCCCCCCHHHHHHEEECEEEEEECCCCHHHHHEEECCCCCCCCCCCCCCCE
VWSGDLVRRDDDGFLYFVARNDAQIKSSGYRISPEEIEEVAHASGLVAEAVALGVPHDEL
EECCCCEEECCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHH
GESITLVVVPLDADTFRPDALRARCAQQLPPYMVPHTIATRTSLPRNPNGKFDRVALRAD
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEHH
AANLVETL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]