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The map label for this gene is proB [H]

Identifier: 75910011

GI number: 75910011

Start: 4741088

End: 4742197

Strand: Direct

Name: proB [H]

Synonym: Ava_3807

Alternate gene names: 75910011

Gene position: 4741088-4742197 (Clockwise)

Preceding gene: 75910010

Following gene: 75910013

Centisome position: 74.48

GC content: 45.77

Gene sequence:

>1110_bases
ATGACCAAAACAATTGTCGTCAAAATCGGCACATCTAGCCTCACCCAACCAGAAACCGGACAACTAGCCCTCTCCACCAT
TGCAACTTTGGCGGAAACACTCTCTTATTTAAGACAGCAAGGTAATCAAGTGATTTTAGTCTCCTCTGGGGCTGTGGGAG
TCGGTTGTGCGCGGTTGGGTTTAACGGAACGTCCCAAAGCGATCGCTCTCAAACAAGCAGTAGCCGCAGTGGGTCAAGGT
AGATTAATGCGCGTATATGACGACTTATTTACCACCTTACAACAGCCGATCGCCCAAGTTTTGCTAACTCGCAGCGATTT
GGTACAACGTAGCCGTTATCTCAACGTATACAACACCTTTCGGGAATTACTTGCCTTGGGGGTAATTCCTGTAGTCAACG
AGAATGATACCGTTGCCGTGGATGAACTGAAATTTGGTGATAATGATACCCTTTCAGCTTTGGTTGCTAGTCTTGTAGAG
GCAGACTGGCTATTTTTACTCACTGACGTAGACCGATTATACTCAGCCGATCCCCGTTCTGTCCCCGATGCCCGACCGAT
ATCTTTGGTGACAAGCATCAAAGAATTAGCAGACTTACAAGTACAAACAGGTTCTCAAGGTTCTCAATGGGGTACTGGGG
GAATGATGACAAAAATTTCGGCGGCGAGAATTGCGATCGCGGCGGGAGTGCGTACTGTGATTACTCAAGGGCGATATCCT
CGCAATATCGAAAAAATTATCCAAGGGGAACTGATTGGAACTCATTTTCAACCCCAACCAGAACCAACCTCCGCCCGTAA
ACGTTGGATTGCTTACGGTTTAGTCCCGACAGGTAAATTATACTTAGATTCTGGTGCGATCGCCGCCATAGTTAAAGCAG
GTAAATCGTTATTGCCAGCAGGAGTTAAAACCGTTGCAGGTGAATTTGAGCCTCAAGATGCAGTACAGCTATTAGATCCT
CAAGGTAACGAAATTGCCAGAGGACTAGTTAACTATAGCAGCAAAGACTTACAGCAAATTTGTGGTCGTCACTCTAAAGA
AATTTCGACTATTTTGGGTTATGTAGGTGCAGAAACCGTGATCCATCGAGATAACTTAGTATTGACGTAG

Upstream 100 bases:

>100_bases
ATAGTAAAAACTGTTGACTGTTGACTGGAAGAGGCAGGGGGAAACCTTCTCCCCATCTCCCTGCCCCCCTGCCCCCTGCC
CCTTTTCCTCTTCTTGACCA

Downstream 100 bases:

>100_bases
GTGTGTACGGAAAAGAGATGTTCTCTTGATTAGCTGACTTAAAAGAGAAAAACCCCGATACCTAACGGGGTTAATGTTAC
TTTAGCCACAAAATTTACCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQG
RLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVE
ADWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLLDP
QGNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETVIHRDNLVLT

Sequences:

>Translated_369_residues
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQG
RLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVE
ADWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLLDP
QGNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETVIHRDNLVLT
>Mature_368_residues
TKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLGLTERPKAIALKQAVAAVGQGR
LMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTFRELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEA
DWLFLLTDVDRLYSADPRSVPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYPR
NIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPAGVKTVAGEFEPQDAVQLLDPQ
GNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETVIHRDNLVLT

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=32.6388888888889, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.5174825174825, Blast_Score=128, Evalue=9e-30,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.4426229508197, Blast_Score=290, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=35.9550561797753, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=35.9550561797753, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=41.1057692307692, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6321822, Length=422, Percent_Identity=38.8625592417062, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI21357643, Length=294, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39704; Mature: 39573

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLG
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LTERPKAIALKQAVAAVGQGRLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEADWLFLLTDVDRLYSADPRS
HHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEECCCCCEEEECCCHHHHHHHHCHHHCCC
GVKTVAGEFEPQDAVQLLDPQGNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETV
HHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHE
IHRDNLVLT
EECCCEEEC
>Mature Secondary Structure 
TKTIVVKIGTSSLTQPETGQLALSTIATLAETLSYLRQQGNQVILVSSGAVGVGCARLG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCC
LTERPKAIALKQAVAAVGQGRLMRVYDDLFTTLQQPIAQVLLTRSDLVQRSRYLNVYNTF
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELLALGVIPVVNENDTVAVDELKFGDNDTLSALVASLVEADWLFLLTDVDRLYSADPRS
HHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPDARPISLVTSIKELADLQVQTGSQGSQWGTGGMMTKISAARIAIAAGVRTVITQGRYP
CCCCCHHHHHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RNIEKIIQGELIGTHFQPQPEPTSARKRWIAYGLVPTGKLYLDSGAIAAIVKAGKSLLPA
HHHHHHHHCCCCCCCCCCCCCCCHHHHHEEEEECCCCCEEEECCCHHHHHHHHCHHHCCC
GVKTVAGEFEPQDAVQLLDPQGNEIARGLVNYSSKDLQQICGRHSKEISTILGYVGAETV
HHHHHCCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHE
IHRDNLVLT
EECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]