Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 75909959

GI number: 75909959

Start: 4675839

End: 4677002

Strand: Direct

Name: tgt [H]

Synonym: Ava_3755

Alternate gene names: 75909959

Gene position: 4675839-4677002 (Clockwise)

Preceding gene: 75909958

Following gene: 75909961

Centisome position: 73.45

GC content: 45.53

Gene sequence:

>1164_bases
TTGTCCATATACTTAAACAAGCTGAATCTTACCTTGAGTGCTATATTTTCTTTTCAATCCCTAGCTCGTTGTAGCCAAAC
AAAAGCCAGAGCAGGGGTATTTTTAACTCCCCACGGTGTTGTAGAAACTCCGAGATTTATGCCGGTGGGAACCCTGGCCA
ATGTAAAGACTGTCACCCCAGCCCAACTGAAGGAGACTGGGGCGCAGATGGTTCTTTCTAATACTTATCATCTCCATCTC
CAACCAGGGGAAGCCATTGTGGCTGGTGGTGGTGGATTGCATAAATTTATGGGCTGGAATGGGCCGATGCTCACCGATTC
TGGTGGGTTTCAGGTTTTCAGCTTGAGCGAAATGAGAAAAATTACGGAAGAGGGTGTAACTTTTCGCTCACCCCACGACG
GGCAAATTATTAAATTAACGCCGGAACGCTCTATAGAGATTCAAAATATTTTAGGGGCTGATGTTATCATGGCGTTCGAT
GAATGTCCCCCCTACCCAGCGACTCGCCAGGAAGTAGAAGCGGCGACTGAGCGGACTTATCGTTGGTTGGAACGTTGTAT
TACGGCGCATCAACGCCAGGATCAAGCGTTGTTTGGTATTGTTCAAGGTGGGGTATATCTGGATTTACGGGCTAAAGCTG
CCAATACTTTAACTGAGCTAGATTTACCTGGTTATGCCATTGGTGGGGTGAGTGTGGGGGAACCACCAGAAATGATGGCG
CAAATTGTCCAAGCTACAGCACCACTGTTACCAGCACACAAGCCCCGCTACTTGATGGGTGTGGGAACTTATCGAGAAAT
GGTAATTGCGATCGCCTCTGGTATAGACTTATTTGATTGCGTCATTCCCACCCGTTGGGCGAGACACGGTACAGCAATGG
TAAAAGGCGAACGTTGGAACTTAAAAAATGCTAAGTTCCGTGAAGATTTTGCCCCCATAGATGAAACTTGCCCTTGTTAT
GCTTGTCAAAATTTCAGCCGTGCCTATATTTCTCATTTAGTGCGATCGCAAGAAATTTTAGCCTACACTTTACTGAGCAT
TCACAATATTACCGAACTCATTCGCTTTACCCAAAAAATTCGTGAAGCAATATTAAGCGATCGTTTTTTGGAAGAATTTG
GTCACTGGTTAAACTCGCCGGAAACTGAAGTTGATAATGGGTAA

Upstream 100 bases:

>100_bases
TGATGACTAATCAGTTACTGTTTATTTATTACCAATTATTTTGGGTAAGTGCTGAGTCAGAATTTCTCCTTTATCTTCTT
CCCCTGCTCCCTACCTATTT

Downstream 100 bases:

>100_bases
TGACGCGATGTGCGACTTATTTTTGAAACCCCTCTCCAAACCTCTCCCCGAAGCGGAGAGAGGCTTTGAATCTTGCTCCC
CTTCCCTGCAAGGTAAGGGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSIYLNKLNLTLSAIFSFQSLARCSQTKARAGVFLTPHGVVETPRFMPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHL
QPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGVTFRSPHDGQIIKLTPERSIEIQNILGADVIMAFD
ECPPYPATRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMA
QIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWARHGTAMVKGERWNLKNAKFREDFAPIDETCPCY
ACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILSDRFLEEFGHWLNSPETEVDNG

Sequences:

>Translated_387_residues
MSIYLNKLNLTLSAIFSFQSLARCSQTKARAGVFLTPHGVVETPRFMPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHL
QPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGVTFRSPHDGQIIKLTPERSIEIQNILGADVIMAFD
ECPPYPATRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMA
QIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWARHGTAMVKGERWNLKNAKFREDFAPIDETCPCY
ACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILSDRFLEEFGHWLNSPETEVDNG
>Mature_386_residues
SIYLNKLNLTLSAIFSFQSLARCSQTKARAGVFLTPHGVVETPRFMPVGTLANVKTVTPAQLKETGAQMVLSNTYHLHLQ
PGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRKITEEGVTFRSPHDGQIIKLTPERSIEIQNILGADVIMAFDE
CPPYPATRQEVEAATERTYRWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMAQ
IVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWARHGTAMVKGERWNLKNAKFREDFAPIDETCPCYA
CQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKIREAILSDRFLEEFGHWLNSPETEVDNG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=47.1428571428571, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=25, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786607, Length=354, Percent_Identity=48.5875706214689, Blast_Score=345, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17544680, Length=349, Percent_Identity=46.1318051575931, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25146502, Length=234, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=47.0422535211268, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI21355311, Length=252, Percent_Identity=23.8095238095238, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43031; Mature: 42900

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYLNKLNLTLSAIFSFQSLARCSQTKARAGVFLTPHGVVETPRFMPVGTLANVKTVTP
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCH
AQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRK
HHHHHHHHHEEEECEEEEEECCCCEEEECCCCHHHHCCCCCCEEECCCCEEEEEHHHHHH
ITEEGVTFRSPHDGQIIKLTPERSIEIQNILGADVIMAFDECPPYPATRQEVEAATERTY
HHHCCCEECCCCCCEEEEECCCCCEEHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHHHH
RWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMA
HHHHHHHHHHHCCCHHHHEEECCCEEEEEEHHHHCHHHHCCCCCEEECCCCCCCCHHHHH
QIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWARHGTAMVKGERWN
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCHHHHHCCCEEEECCCCC
LKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKI
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAILSDRFLEEFGHWLNSPETEVDNG
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SIYLNKLNLTLSAIFSFQSLARCSQTKARAGVFLTPHGVVETPRFMPVGTLANVKTVTP
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCH
AQLKETGAQMVLSNTYHLHLQPGEAIVAGGGGLHKFMGWNGPMLTDSGGFQVFSLSEMRK
HHHHHHHHHEEEECEEEEEECCCCEEEECCCCHHHHCCCCCCEEECCCCEEEEEHHHHHH
ITEEGVTFRSPHDGQIIKLTPERSIEIQNILGADVIMAFDECPPYPATRQEVEAATERTY
HHHCCCEECCCCCCEEEEECCCCCEEHHHHHCHHHEEEECCCCCCCCCHHHHHHHHHHHH
RWLERCITAHQRQDQALFGIVQGGVYLDLRAKAANTLTELDLPGYAIGGVSVGEPPEMMA
HHHHHHHHHHHCCCHHHHEEECCCEEEEEEHHHHCHHHHCCCCCEEECCCCCCCCHHHHH
QIVQATAPLLPAHKPRYLMGVGTYREMVIAIASGIDLFDCVIPTRWARHGTAMVKGERWN
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCHHHHHCCCEEEECCCCC
LKNAKFREDFAPIDETCPCYACQNFSRAYISHLVRSQEILAYTLLSIHNITELIRFTQKI
CCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAILSDRFLEEFGHWLNSPETEVDNG
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]