Click here to switch to the map view.

The map label for this gene is dnaE-N [H]

Identifier: 75909952

GI number: 75909952

Start: 4665452

End: 4666804

Strand: Direct

Name: dnaE-N [H]

Synonym: Ava_3748

Alternate gene names: 75909952

Gene position: 4665452-4666804 (Clockwise)

Preceding gene: 75909950

Following gene: 75909953

Centisome position: 73.29

GC content: 38.88

Gene sequence:

>1353_bases
ATGATAAAAATAGCAAGCCGTAAGTTTTTGGGTGTAGAAAATGTCTATGACATTGGGGTTGGACGTGATCACAATTTTTT
CGTCAAAAATGGATTAATAGCTTCTAATTGTTTCAATAAATCCCATTCCACAGCTTATGGATATGTTACTTATCAAACTG
CATATCTAAAAGCTAATTATCCATTGGAATATATGGCGGCGTTGCTGACGGCTAACAGCAACGATACAGACAAGGTGCAG
AAATATATTTCTACCTGTCTAAGCATGAATATTCAGATAGAACCGCCGGACATCAATCGCTCAGGTGTAGACTTTACCCC
AGTCGCGGGTAAGATATTATTTGGATTTTCGGCTGTGAGAAACGTAGGACAGAATGCGATCGCCTCAGTTTTAGAAGCAA
GAGAGACAACAGGAGAGTTTAAATCATTAGCTGATTTCTGCGATCGCGTAGACTTGCGTGCTGTTAATCGCCGCACCCTT
GAATCACTAATTTACTGTGGAGCCTTTGACAAAATTGAAGCGAATCGTCACCAATTAATCCAAGATTTAGAATTAGTCTA
TGATTGGGCCCAATCCCGTGCTAGAGATCGTGCTAGTGGTCAAGGAAACCTGTTTGATTTATTAGGTGGATATTCTTCTA
CTAGTACCAAAACAGCTAATAATGCCTTTGAAACTGCTCCTAAAGCTAAACCTGTACTAGATTATCCCCCACAGGAAAAA
TTACGCTTAGAAAAAGAATTATTAGGATTTTATGTCTCCGACCATCCCTTAAAATCTTTAAGGTCAGTAGCACCACTTTT
AACACCAATTAATCTTTCACAACTAGGAGAACAACGAGAAGACACAAGATTGTGTGTGATTGTCATGCTTAACGGTGTAA
AAAAAGTCATGACAAAGAAAGGCGATGCAATGGCAATTCTGCAACTTGAAGACTTAACATCTCAATCAGAAGCAGTAGTT
TTTCCGAAAACTTATGAGCGCATTAGTTTCTTACTTCAAGTAGATGCCAGATTAATTATTTGGGGTAAAGTAGACCGACG
AGACGAACAAACTCAATTAATTGTAGAAGATGCAGAGCCAGTAGAAACTGTACAAATGGTGATGGTAGAACTTCAGCCTC
AACAAGCAGCCAATATTGAAGAACTACACCGCTTAAAAACAATTTTGCAAGAACAATGTGGAGATAAAGAAAAAGCCAAA
ATGCCCATAATTGGAATTGTGCAATCTGAAAATTCTCGGCGATTAGTGCGCTTAGGTTGGCAATTTTGTGTTCAAGATGC
TCGAATTACCGTGCAAGCATTACAAAATGCGAGATTTCCAGCACAACTTAAACCTCTGATTGGTGGTTCATGA

Upstream 100 bases:

>100_bases
CGCCAATAAATTATATAAGATGGATTTTAGTAAAAGTTAAATTTTAGCTAAATATAGAAATTAATTGCTTAACTAGCAAA
TTATTTTGAGGTATAAATAG

Downstream 100 bases:

>100_bases
GGAGATGAAATAGGAAGTGAGAAGAGGCAATACTTCTCTGCGTTCTTGAGTGTGTCGTAGGCTCAGTAACAAGGTGTAGG
GGAAAGAATATCTTACTCAG

Product: DNA polymerase III subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MIKIASRKFLGVENVYDIGVGRDHNFFVKNGLIASNCFNKSHSTAYGYVTYQTAYLKANYPLEYMAALLTANSNDTDKVQ
KYISTCLSMNIQIEPPDINRSGVDFTPVAGKILFGFSAVRNVGQNAIASVLEARETTGEFKSLADFCDRVDLRAVNRRTL
ESLIYCGAFDKIEANRHQLIQDLELVYDWAQSRARDRASGQGNLFDLLGGYSSTSTKTANNAFETAPKAKPVLDYPPQEK
LRLEKELLGFYVSDHPLKSLRSVAPLLTPINLSQLGEQREDTRLCVIVMLNGVKKVMTKKGDAMAILQLEDLTSQSEAVV
FPKTYERISFLLQVDARLIIWGKVDRRDEQTQLIVEDAEPVETVQMVMVELQPQQAANIEELHRLKTILQEQCGDKEKAK
MPIIGIVQSENSRRLVRLGWQFCVQDARITVQALQNARFPAQLKPLIGGS

Sequences:

>Translated_450_residues
MIKIASRKFLGVENVYDIGVGRDHNFFVKNGLIASNCFNKSHSTAYGYVTYQTAYLKANYPLEYMAALLTANSNDTDKVQ
KYISTCLSMNIQIEPPDINRSGVDFTPVAGKILFGFSAVRNVGQNAIASVLEARETTGEFKSLADFCDRVDLRAVNRRTL
ESLIYCGAFDKIEANRHQLIQDLELVYDWAQSRARDRASGQGNLFDLLGGYSSTSTKTANNAFETAPKAKPVLDYPPQEK
LRLEKELLGFYVSDHPLKSLRSVAPLLTPINLSQLGEQREDTRLCVIVMLNGVKKVMTKKGDAMAILQLEDLTSQSEAVV
FPKTYERISFLLQVDARLIIWGKVDRRDEQTQLIVEDAEPVETVQMVMVELQPQQAANIEELHRLKTILQEQCGDKEKAK
MPIIGIVQSENSRRLVRLGWQFCVQDARITVQALQNARFPAQLKPLIGGS
>Mature_450_residues
MIKIASRKFLGVENVYDIGVGRDHNFFVKNGLIASNCFNKSHSTAYGYVTYQTAYLKANYPLEYMAALLTANSNDTDKVQ
KYISTCLSMNIQIEPPDINRSGVDFTPVAGKILFGFSAVRNVGQNAIASVLEARETTGEFKSLADFCDRVDLRAVNRRTL
ESLIYCGAFDKIEANRHQLIQDLELVYDWAQSRARDRASGQGNLFDLLGGYSSTSTKTANNAFETAPKAKPVLDYPPQEK
LRLEKELLGFYVSDHPLKSLRSVAPLLTPINLSQLGEQREDTRLCVIVMLNGVKKVMTKKGDAMAILQLEDLTSQSEAVV
FPKTYERISFLLQVDARLIIWGKVDRRDEQTQLIVEDAEPVETVQMVMVELQPQQAANIEELHRLKTILQEQCGDKEKAK
MPIIGIVQSENSRRLVRLGWQFCVQDARITVQALQNARFPAQLKPLIGGS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=319, Percent_Identity=32.9153605015674, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR003587
- InterPro:   IPR006141
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 50462; Mature: 50462

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50818 INTEIN_C_TER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIASRKFLGVENVYDIGVGRDHNFFVKNGLIASNCFNKSHSTAYGYVTYQTAYLKANY
CEEECCCCCCCCCCCEECCCCCCCCEEEECCEEEHHCCCCCCCCEEEEEEEEEEEEEECC
PLEYMAALLTANSNDTDKVQKYISTCLSMNIQIEPPDINRSGVDFTPVAGKILFGFSAVR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
NVGQNAIASVLEARETTGEFKSLADFCDRVDLRAVNRRTLESLIYCGAFDKIEANRHQLI
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHH
QDLELVYDWAQSRARDRASGQGNLFDLLGGYSSTSTKTANNAFETAPKAKPVLDYPPQEK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHH
LRLEKELLGFYVSDHPLKSLRSVAPLLTPINLSQLGEQREDTRLCVIVMLNGVKKVMTKK
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEHHHHHHHHHCC
GDAMAILQLEDLTSQSEAVVFPKTYERISFLLQVDARLIIWGKVDRRDEQTQLIVEDAEP
CCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCH
VETVQMVMVELQPQQAANIEELHRLKTILQEQCGDKEKAKMPIIGIVQSENSRRLVRLGW
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
QFCVQDARITVQALQNARFPAQLKPLIGGS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure
MIKIASRKFLGVENVYDIGVGRDHNFFVKNGLIASNCFNKSHSTAYGYVTYQTAYLKANY
CEEECCCCCCCCCCCEECCCCCCCCEEEECCEEEHHCCCCCCCCEEEEEEEEEEEEEECC
PLEYMAALLTANSNDTDKVQKYISTCLSMNIQIEPPDINRSGVDFTPVAGKILFGFSAVR
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
NVGQNAIASVLEARETTGEFKSLADFCDRVDLRAVNRRTLESLIYCGAFDKIEANRHQLI
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCHHHHHH
QDLELVYDWAQSRARDRASGQGNLFDLLGGYSSTSTKTANNAFETAPKAKPVLDYPPQEK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHH
LRLEKELLGFYVSDHPLKSLRSVAPLLTPINLSQLGEQREDTRLCVIVMLNGVKKVMTKK
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEEHHHHHHHHHCC
GDAMAILQLEDLTSQSEAVVFPKTYERISFLLQVDARLIIWGKVDRRDEQTQLIVEDAEP
CCEEEEEEECCCCCCCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCH
VETVQMVMVELQPQQAANIEELHRLKTILQEQCGDKEKAKMPIIGIVQSENSRRLVRLGW
HHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHH
QFCVQDARITVQALQNARFPAQLKPLIGGS
HHHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231; 9689062; 10734038; 11170467 [H]