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The map label for this gene is gatA [H]

Identifier: 75909879

GI number: 75909879

Start: 4579353

End: 4580747

Strand: Direct

Name: gatA [H]

Synonym: Ava_3674

Alternate gene names: 75909879

Gene position: 4579353-4580747 (Clockwise)

Preceding gene: 75909878

Following gene: 75909880

Centisome position: 71.94

GC content: 47.89

Gene sequence:

>1395_bases
ATGAATGATGCTGTTTCCATCGCCAAGGCTATAGGTGAAGGTAAAGTTACCGCCGTAGAGGTAACCCAGGCTGCTTTAGC
GCGGATAGCAGCACAGGATGTAGAACTGAATTGTTTTACGGCTGTAACTAGAGAAACGGCTTTGGCGGATGCAGCACGTA
TTGATAGGGAAATAGCCGCAGGTAATGATTCTGGGTTACTGGCTGGTGTGCCTTTTGCTGTGAAAAACTTATTTGATCTT
GTCGGTTTGACGACTCTGGCGGGGGCAAAAATCAATGCCGAAAATCCGCCAGCTAGTCGAGATGCAACCGCAGTAGCCAA
GCTGAAACAAGCTGGTGCGGTGTTGGTAGGCGCTTTAAATATGGATGAGTACGCCTACGGATTTGTTACAGAAAATTCCC
ATTATGGTGTTACCCGCAACCCCCATGATTTACAGCGAGTGGCTGGGGGTTCCTCTGGCGGTTCGGCGGTGGCTGTGGCG
GCTGGGTTGGTTCCTTTTACCTTGGGTTCTGATACTAACGGTTCCATTCGTGTCCCGGCGGCTTTGTGTGGTGTGTTTGG
TTTCAAACCGACTTACGGGCGTTTGTCTCGTGGTGGGGTGACTTTATTTTCTAGTAGTTTTGACCACATCGGCCCCTTTG
CCCGTTCGGTTGGGGATATCGCCACAATATTTGATATTTTGCAAGGAGGAGATGATCGCGATCCTATCTGCACTCAACGA
CCTGCAAACCCCACTTTACCGCAACTGCATCAAGATATCTCTCATATTAGAATAGCGATCGCTGACGATTATTTCCAAAA
AGGGGCGACATCGGCAGCATTAGCAGCCGTACAGCAGGTAGCAGATGCTTTAAACGTAACCAATTACGTAACTTTACCAG
AAGCCCACCGCGCCAGGGCAGCCGCATTTGTCATTACCGCCAGCGAAGGCGCAAATTTACACTTAGATAAATTAAGAACA
CGTTCCCAAGACTTTGACCCAGCAACACGCGATCGCTTTTTGGCAGGGGCATTAATTCCCAGTAATTGGTATATACAAGC
ACAACGCTTTCGGCAATGGTACCGCGATCGCATCCGTGAAGTTTTTCAGAATGTTGATGTAATTCTTGCCCCTACCACAC
CCATTACAGCACCCCTAATCGGTCAACAGACAATGATTTTAGATGGGGAGGAAATTCTAGTGCGTCCCCACTTAGGATTG
TTCACTCAACCCTTATCTTTTATTGGTTTACCAGTTTTATCAGTACCAATTCAGCGTCCCAATACTTTACCTTTGGGTGT
GCAGTTAATCGCCGCCCCATACAATGAAGCCTTCATTTTACAAGTGGCGGCTGTATTAGAAGCCCAAGGTGTAGTTTCAG
CCGTGAGTCCGATAAATTATAACTCCTCTCCGTAG

Upstream 100 bases:

>100_bases
ATGGAGTGGTGGCAAATTTTGAGAGAATAAGAGCGATCGCTCAACTTGTTAACTCATTTCCACTACCAGAAGCAATAGAA
CCCGCCCCTGTCTTTGAACC

Downstream 100 bases:

>100_bases
CTTGCTTGTCGATGCAAGGAGAGGCTTAAAACTCTAATTCTTTGATACTCAGTTATATGCTTTTGCCCCTTCCCTTGTAG
GATACTGTTGGCGAATTATT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNDAVSIAKAIGEGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDREIAAGNDSGLLAGVPFAVKNLFDL
VGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGVTRNPHDLQRVAGGSSGGSAVAVA
AGLVPFTLGSDTNGSIRVPAALCGVFGFKPTYGRLSRGGVTLFSSSFDHIGPFARSVGDIATIFDILQGGDDRDPICTQR
PANPTLPQLHQDISHIRIAIADDYFQKGATSAALAAVQQVADALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RSQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQRPNTLPLGVQLIAAPYNEAFILQVAAVLEAQGVVSAVSPINYNSSP

Sequences:

>Translated_464_residues
MNDAVSIAKAIGEGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDREIAAGNDSGLLAGVPFAVKNLFDL
VGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGVTRNPHDLQRVAGGSSGGSAVAVA
AGLVPFTLGSDTNGSIRVPAALCGVFGFKPTYGRLSRGGVTLFSSSFDHIGPFARSVGDIATIFDILQGGDDRDPICTQR
PANPTLPQLHQDISHIRIAIADDYFQKGATSAALAAVQQVADALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RSQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQRPNTLPLGVQLIAAPYNEAFILQVAAVLEAQGVVSAVSPINYNSSP
>Mature_464_residues
MNDAVSIAKAIGEGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDREIAAGNDSGLLAGVPFAVKNLFDL
VGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGVTRNPHDLQRVAGGSSGGSAVAVA
AGLVPFTLGSDTNGSIRVPAALCGVFGFKPTYGRLSRGGVTLFSSSFDHIGPFARSVGDIATIFDILQGGDDRDPICTQR
PANPTLPQLHQDISHIRIAIADDYFQKGATSAALAAVQQVADALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RSQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQRPNTLPLGVQLIAAPYNEAFILQVAAVLEAQGVVSAVSPINYNSSP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=29.4, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=27.710843373494, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI166795287, Length=185, Percent_Identity=34.5945945945946, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=30.2884615384615, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17556264, Length=193, Percent_Identity=36.2694300518135, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=23.3870967741935, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=267, Percent_Identity=31.0861423220974, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=267, Percent_Identity=31.0861423220974, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=307, Percent_Identity=26.0586319218241, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=374, Percent_Identity=24.5989304812834, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=386, Percent_Identity=32.1243523316062, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323950, Length=398, Percent_Identity=28.1407035175879, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=27.3127753303965, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=33.265306122449, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24644968, Length=495, Percent_Identity=26.8686868686869, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=26.0606060606061, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=26.0606060606061, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=26.0606060606061, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=26.0606060606061, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=33.6170212765957, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=26.6802443991853, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=26.6802443991853, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=26.6802443991853, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078093, Length=482, Percent_Identity=27.1784232365145, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648441, Length=419, Percent_Identity=26.0143198090692, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648439, Length=419, Percent_Identity=26.0143198090692, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49155; Mature: 49155

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAVSIAKAIGEGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDREIAA
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
GNDSGLLAGVPFAVKNLFDLVGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALN
CCCCCEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTENSHYGVTRNPHDLQRVAGGSSGGSAVAVAAGLVPFTLGSDTNGSIRVPA
CCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCEECHH
ALCGVFGFKPTYGRLSRGGVTLFSSSFDHIGPFARSVGDIATIFDILQGGDDRDPICTQR
HHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PANPTLPQLHQDISHIRIAIADDYFQKGATSAALAAVQQVADALNVTNYVTLPEAHRARA
CCCCCHHHHHHHHHHEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHCCE
AAFVITASEGANLHLDKLRTRSQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIRE
EEEEEEECCCCCEEHHHHHHCCCCCCCHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHH
VFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGLFTQPLSFIGLPVLSVPIQRP
HHCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHHHCCCHHEECCCCC
NTLPLGVQLIAAPYNEAFILQVAAVLEAQGVVSAVSPINYNSSP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNDAVSIAKAIGEGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDREIAA
CCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
GNDSGLLAGVPFAVKNLFDLVGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALN
CCCCCEEEECCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTENSHYGVTRNPHDLQRVAGGSSGGSAVAVAAGLVPFTLGSDTNGSIRVPA
CCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCEECHH
ALCGVFGFKPTYGRLSRGGVTLFSSSFDHIGPFARSVGDIATIFDILQGGDDRDPICTQR
HHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PANPTLPQLHQDISHIRIAIADDYFQKGATSAALAAVQQVADALNVTNYVTLPEAHRARA
CCCCCHHHHHHHHHHEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHCCE
AAFVITASEGANLHLDKLRTRSQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIRE
EEEEEEECCCCCEEHHHHHHCCCCCCCHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHH
VFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGLFTQPLSFIGLPVLSVPIQRP
HHCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHHHCCCHHEECCCCC
NTLPLGVQLIAAPYNEAFILQVAAVLEAQGVVSAVSPINYNSSP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA