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The map label for this gene is rsmF [H]

Identifier: 75909870

GI number: 75909870

Start: 4571951

End: 4572919

Strand: Direct

Name: rsmF [H]

Synonym: Ava_3665

Alternate gene names: 75909870

Gene position: 4571951-4572919 (Clockwise)

Preceding gene: 75909867

Following gene: 75909872

Centisome position: 71.82

GC content: 36.95

Gene sequence:

>969_bases
ATGGAAAAACCATCGAACTTACTGCTTAAACTTGCACGACGTTTGTTTGAAAATCCTGATAAACAAGAAAAATTTATCGA
TGCGTTAGTTGCTCCCCAGCCTTTTAATCCCTGTATTCTGTGGTGTCAAAATCAGCCTGATATTTCACCTTTTTCCGTAG
AATCTCCGCTAAGTTGGCAACCGAAATTTGTTGACCGTCTGCACCTGGGAGAAAAACCCGGACAGCATACCTTACATCAT
CAAGGATATTTCTATTGTTTAGATTTTTCATCTGTATTTGCAGCGTCTATTTTATTAACAATTCCTCAATCAGTAAGCTT
AGTTTTTGATATGTGCGCTGCTCCCGGCGGTAAAAGTGTTTTTGCCTGGCAAGCTTTACACCCAGATTTAATCATTTGTA
ATGAAGTGATTGGTAAACGTTTAGGAATGTTAATTTCTAATTTAAAACGTTGTCAAATTAGCCCTTCTATTATATTTAAT
AGAGATTCTAGTATTTTTGCGGAAAATATTCCTTTATCTAGTAATTTAGTTATAGTTGATGCCCCCTGTACTGGGCAATC
TCTATTAGCAAAAGGAGAGAAAGCACCCGGATGTTTTCATCCTACTGCTATTAACAAGAGTGCAAATCGCCAAAAAAGAA
TCATCGCTAATTCTGCACAAATTGTTGCTCCACAAGGTTATCTTGCTTATATGACTTGTACTTATTCCCCTGAAGAGAAT
GAGCAGGTGTGTGAATGGTTTCTGTCACGTTTTCCCCAGTTTCAACCAGTAAGAATTAACCATTTACAAGAGTGTCAGTC
CCACTTAACAAATATTCCTTGTTATCGGTTATTCCCCCAAGATAAACTAGGTGCTGGGGCGTTTACTGTACTTTTTCAAA
ATACTGAAGTTGGAGCAGTTAAAGAATTAGAGCCACAGATTTTAAATGATTTAGGTATTATTCAAAAATTCTCACCCCTT
GTATCCTAA

Upstream 100 bases:

>100_bases
AGTCAGCAATCAACAGTTCTTCCCCGTCTCTATTCCCCATTCCCTAGTATTATGAAGCCAAGCCACTTCTAAGCGTTGTT
CTCCTGTTACCGTTTTACTA

Downstream 100 bases:

>100_bases
TGAAGTCAGGATGATTAATGAATATACAAATTATCAAAGGACACAATATTTGCGTCCCTTGAGGTTGTATTAGAGGATAA
TTCGTCTATCGGTTATTATC

Product: Fmu (Sun)/nucleolar NOL1/Nop2p

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDISPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPDLIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPQFQPVRINHLQECQSHLTNIPCYRLFPQDKLGAGAFTVLFQNTEVGAVKELEPQILNDLGIIQKFSPL
VS

Sequences:

>Translated_322_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDISPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPDLIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPQFQPVRINHLQECQSHLTNIPCYRLFPQDKLGAGAFTVLFQNTEVGAVKELEPQILNDLGIIQKFSPL
VS
>Mature_322_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDISPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPDLIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPQFQPVRINHLQECQSHLTNIPCYRLFPQDKLGAGAFTVLFQNTEVGAVKELEPQILNDLGIIQKFSPL
VS

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=174, Percent_Identity=28.1609195402299, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI76150623, Length=174, Percent_Identity=28.1609195402299, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI11545785, Length=222, Percent_Identity=27.4774774774775, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87081985, Length=228, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=174, Percent_Identity=27.0114942528736, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319447, Length=160, Percent_Identity=27.5, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI22024126, Length=174, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355201, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19920494, Length=158, Percent_Identity=29.1139240506329, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 36013; Mature: 36013

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDISPFSVESPLSWQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKFVDRLHLGEKPGQHTLHHQGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSV
HHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
FAWQALHPDLIICNEVIGKRLGMLISNLKRCQISPSIIFNRDSSIFAENIPLSSNLVIVD
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEEE
APCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
CCCCCHHHHHCCCCCCCCCCCHHCCCCCCHHHHHEECCCEEECCCCEEEEEEEECCCCCC
EQVCEWFLSRFPQFQPVRINHLQECQSHLTNIPCYRLFPQDKLGAGAFTVLFQNTEVGAV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCH
KELEPQILNDLGIIQKFSPLVS
HHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDISPFSVESPLSWQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKFVDRLHLGEKPGQHTLHHQGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSV
HHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
FAWQALHPDLIICNEVIGKRLGMLISNLKRCQISPSIIFNRDSSIFAENIPLSSNLVIVD
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEEE
APCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
CCCCCHHHHHCCCCCCCCCCCHHCCCCCCHHHHHEECCCEEECCCCEEEEEEEECCCCCC
EQVCEWFLSRFPQFQPVRINHLQECQSHLTNIPCYRLFPQDKLGAGAFTVLFQNTEVGAV
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEECCCCCCH
KELEPQILNDLGIIQKFSPLVS
HHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA