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The map label for this gene is purF [H]

Identifier: 75909842

GI number: 75909842

Start: 4535343

End: 4536842

Strand: Direct

Name: purF [H]

Synonym: Ava_3637

Alternate gene names: 75909842

Gene position: 4535343-4536842 (Clockwise)

Preceding gene: 75909841

Following gene: 75909848

Centisome position: 71.25

GC content: 45.13

Gene sequence:

>1500_bases
ATGATTCCCATTGATTCCGTCACTCCGGATGAGTACCCCAATCAGACTAACAACCCCATCGATAGTCAAGAAAATCGTCC
TGACAAGCCAGAAGAGGCTTGTGGAGTTTTCGGTATTTACGCCCCAGGAGAAAATGTTGCAAAACTAACCTACTTTGGAT
TGTATGCCCTGCAACATCGGGGTCAAGAATCAGCTGGTATTGCCACGTTTGAGGGGACAACAGTTCACCTACATAAAGAC
ATGGGTTTGGTTTCTCAAGTCTTTAATGAGTCGATTCTAGAAGAGTTGCCTGGTGATATCGGTGTTGGTCATACCCGTTA
CTCTACTACCGGTTCCAGCCGCAAAGTTAATGCCCAACCTGCTGTCCTGGATACTCGTTTAGGTAAGTTAGCTTTAGCAC
ACAATGGCAATTTAGTCAATACTGTGAAGTTGCGAGAAGAGCTAGTTAATAGTAACTGCAACCTCGTGACAACCACAGAC
TCAGAAATGATTGCCTTTGCGATCGCCCAAGCAGTCAACGCAGGTGCAGACTGGTTAGATGGGGCGATTCAAGCCTTTCA
TCGTTGCGAGGGAGCTTTTAGCTTAGTTATTGGCACTCCCGTTGGTGTGATGGGGGTGCGTGACACCAACGGCATTCGTC
CTCTGGTCATTGGGACTTTGCCTGGTAATCCAGTGCGTTATGTTTTATCTTCGGAAACCTGTGGTTTAGATATTATTGGC
GCTGATTATCTCCGCGACGTAGAACCAGGCGAAGTAGTTTGGATTACCGAAGAAGGTTTAGCTTCCTACCATTGGAGCCA
AAAACCAGAGCGAAGATTGTGTATTTTCGAGATGATTTATTTTGCTCGTCCCGATAGCCAAATGCACAACGAGACTTTAT
ACAGTTATCGGATGCGTTTAGGACGACAGCTAGCAGCCGAATCCTACGTAGATGCTGATATTGTCTTTGGTGTCCCTGAC
TCTGGAATTCCTGCTGCCATTGGATTTTCTCAGGCTTCTGGTGTAGCCTACGGTGAAGGGTTGATTAAAAATCGCTACGT
TGGTCGTACCTTTATTCAGCCTACCCAATCCATGCGCGAGTCAGGGATTAAAATGAAACTTAACCCCCTCAAAGATGTAC
TGGCGGGTAAAAGAGTAATTATTGTCGATGACTCCATTGTCAGGGGAACTACTAGCCGTAAACTAGTTAAAGCCTTACGT
GATGCTGGTGCGGCAGAAGTACATATGCGGATTTCTTCCCCACCAGTAACCCACCCTTGTTTCTACGGAATTGATACTGA
TACTCAGGATCAGTTAATTGCTGCTACTAAGTCAGTAGAAGAAATTGCCAAGCAATTAGAAGTAGAAAGCCTCGCCTATC
TCAGTTGGGATGGGATGTTAGAAGCCACAAGAGAAGATAAAAATAGTTTCTGTTCTGCCTGTTTTACTGGTGATTACCCA
GTTACTATTCCTGAGCAAGTGAAGCGGTCTAAGTTGATTTTAGAAAAGGTAGTAGTTTAG

Upstream 100 bases:

>100_bases
TATGTTAAGAAAATTCCGCAACTATCCCTAGACATAATCCCAGTGACCCAATAGTTACGAATTATTTTGACTTGACACCC
CAACCAGGAGCAAAGCTAGC

Downstream 100 bases:

>100_bases
GGATTAGAAACAAATCAGTGCTGGGTTTTGAGAATTGACTCAGCACTGTCTTCAGATTACCCAAGCTAAAATGGCTTATG
AACCAGCCTGTTACTTGGTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLREELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGTLPGNPVRYVLSSETCGLDIIG
ADYLRDVEPGEVVWITEEGLASYHWSQKPERRLCIFEMIYFARPDSQMHNETLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQSMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDTQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VTIPEQVKRSKLILEKVVV

Sequences:

>Translated_499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLREELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGTLPGNPVRYVLSSETCGLDIIG
ADYLRDVEPGEVVWITEEGLASYHWSQKPERRLCIFEMIYFARPDSQMHNETLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQSMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDTQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VTIPEQVKRSKLILEKVVV
>Mature_499_residues
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHRGQESAGIATFEGTTVHLHKD
MGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQPAVLDTRLGKLALAHNGNLVNTVKLREELVNSNCNLVTTTD
SEMIAFAIAQAVNAGADWLDGAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGTLPGNPVRYVLSSETCGLDIIG
ADYLRDVEPGEVVWITEEGLASYHWSQKPERRLCIFEMIYFARPDSQMHNETLYSYRMRLGRQLAAESYVDADIVFGVPD
SGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQSMRESGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALR
DAGAAEVHMRISSPPVTHPCFYGIDTDTQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
VTIPEQVKRSKLILEKVVV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=40.9090909090909, Blast_Score=367, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=38.3795309168444, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1790167, Length=190, Percent_Identity=32.1052631578947, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=42.1161825726141, Blast_Score=338, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6323958, Length=476, Percent_Identity=35.9243697478992, Blast_Score=259, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24659598, Length=520, Percent_Identity=40.1923076923077, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=42.7698574338086, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24659604, Length=467, Percent_Identity=42.6124197002141, Blast_Score=376, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54565; Mature: 54565

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GQESAGIATFEGTTVHLHKDMGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQP
CCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCC
AVLDTRLGKLALAHNGNLVNTVKLREELVNSNCNLVTTTDSEMIAFAIAQAVNAGADWLD
CHHHHHHCEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHH
GAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGTLPGNPVRYVLSSETCGLDIIG
HHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEC
ADYLRDVEPGEVVWITEEGLASYHWSQKPERRLCIFEMIYFARPDSQMHNETLYSYRMRL
HHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GRQLAAESYVDADIVFGVPDSGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQSMRE
HHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCHHHCCHHHHHH
SGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALRDAGAAEVHMRISSPPVTHPC
CCCEEEECHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
FYGIDTDTQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHEECCCCC
VTIPEQVKRSKLILEKVVV
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPIDSVTPDEYPNQTNNPIDSQENRPDKPEEACGVFGIYAPGENVAKLTYFGLYALQHR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GQESAGIATFEGTTVHLHKDMGLVSQVFNESILEELPGDIGVGHTRYSTTGSSRKVNAQP
CCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEECCCC
AVLDTRLGKLALAHNGNLVNTVKLREELVNSNCNLVTTTDSEMIAFAIAQAVNAGADWLD
CHHHHHHCEEEEECCCCEEEHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHHHH
GAIQAFHRCEGAFSLVIGTPVGVMGVRDTNGIRPLVIGTLPGNPVRYVLSSETCGLDIIG
HHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCEEEEC
ADYLRDVEPGEVVWITEEGLASYHWSQKPERRLCIFEMIYFARPDSQMHNETLYSYRMRL
HHHHCCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GRQLAAESYVDADIVFGVPDSGIPAAIGFSQASGVAYGEGLIKNRYVGRTFIQPTQSMRE
HHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCHHHHCCCCCCHHHCCHHHHHH
SGIKMKLNPLKDVLAGKRVIIVDDSIVRGTTSRKLVKALRDAGAAEVHMRISSPPVTHPC
CCCEEEECHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCE
FYGIDTDTQDQLIAATKSVEEIAKQLEVESLAYLSWDGMLEATREDKNSFCSACFTGDYP
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHEECCCCC
VTIPEQVKRSKLILEKVVV
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]