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The map label for this gene is fmt [H]

Identifier: 75909687

GI number: 75909687

Start: 4315748

End: 4316752

Strand: Direct

Name: fmt [H]

Synonym: Ava_3481

Alternate gene names: 75909687

Gene position: 4315748-4316752 (Clockwise)

Preceding gene: 75909685

Following gene: 75909689

Centisome position: 67.8

GC content: 43.58

Gene sequence:

>1005_bases
ATGAAAATTGTATTTTTTGGTACACCTGAATTTGCTGTTCCTACTTTAGAAAAACTGTTACTGAATCAAGAATTTGAGGT
TTTAGCCGTAGTTACGCAACCGGATAAACGTCGAGAGAGAGGGAATAAATTAACGCCTTCGCCTGTCAAAAATATGGCGA
TCGCTCATGATTTACCAGTATGGCAACCGGAAAGGATAAAAAAAGATACTGAAACTCTCAACAAACTTAAGCAATTAGAC
GCAGATGCGTTTGTTGTAGTTGCCTATGGGCAAATTTTGTCACAAAAAATCCTCGATATGCCCAAATTAGGTTGCGTGAA
CGTACATGGCTCAATTTTGCCCCAGTACCGAGGTGCTGCACCTATTCAATGGTGTTTATATAACGGCGAAACAGAAACGG
GGATCACGACAATGTTAATGGATGCCGGTATGGATACTGGGGCAATGCTACTGAAAGCAACCACACCCATAGGCTTGCTT
GATAATGCTGATGATGTAGCCCAGAGGCTATCTGTGATTGGTGGAGATTTATTAATAGAAACCTTGTACAAGCTACAGCA
ACAGGAAATTCAACCAATCCCGCAAGATAATGCTGCTGCCACTTACGCCTCTTTAATTCAAAAACAAGATTATGGTTTAG
ATTGGTCAAGGAGCGCCTTGCAACTGCACAATCAAATTAGGGGATTTTACCCAAATTGTACAACTACCTTCCGGAACCAA
CCTCTGAAGATTACAGCCTCCTTTCCCCTTGGTGCTGCATACAATGACGAGCTACCACCAGAATTACAAAAAATGTTGCA
AAAACTACCTGATTTATCACAGATATCGGGTAGCCCAGGAGAAGTAGTCAGCATAACCAAGGGAGTTGGTGCGATCGCTC
AAACTGGTGAGGGACTTTTACTTTTACGGGAGGTTCAGCTACCTGGCAAACGCCCCCAGTCGGGCTGGGATTTTGTGAAT
GGTACGCGGCTGACGGTGGGAGAAGTGTTAGGAAATGGCAGCTAG

Upstream 100 bases:

>100_bases
TACTGACAACTGTACGGGAGGGTTTACAGATATCCTCGCTCTCGGACGATGATCTTTATAAACCCGCCCCTACTGACAAC
TGACTATTGACTAATAACTA

Downstream 100 bases:

>100_bases
TGAGGTTTCATAAGAACGGCAGGACTCACAAGGGCCGTGGGGATTGACTGCACAGCGAATGATTTCTGAGTGGGCATTAA
ATCTACAGGTGGCATCACCT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKIVFFGTPEFAVPTLEKLLLNQEFEVLAVVTQPDKRRERGNKLTPSPVKNMAIAHDLPVWQPERIKKDTETLNKLKQLD
ADAFVVVAYGQILSQKILDMPKLGCVNVHGSILPQYRGAAPIQWCLYNGETETGITTMLMDAGMDTGAMLLKATTPIGLL
DNADDVAQRLSVIGGDLLIETLYKLQQQEIQPIPQDNAAATYASLIQKQDYGLDWSRSALQLHNQIRGFYPNCTTTFRNQ
PLKITASFPLGAAYNDELPPELQKMLQKLPDLSQISGSPGEVVSITKGVGAIAQTGEGLLLLREVQLPGKRPQSGWDFVN
GTRLTVGEVLGNGS

Sequences:

>Translated_334_residues
MKIVFFGTPEFAVPTLEKLLLNQEFEVLAVVTQPDKRRERGNKLTPSPVKNMAIAHDLPVWQPERIKKDTETLNKLKQLD
ADAFVVVAYGQILSQKILDMPKLGCVNVHGSILPQYRGAAPIQWCLYNGETETGITTMLMDAGMDTGAMLLKATTPIGLL
DNADDVAQRLSVIGGDLLIETLYKLQQQEIQPIPQDNAAATYASLIQKQDYGLDWSRSALQLHNQIRGFYPNCTTTFRNQ
PLKITASFPLGAAYNDELPPELQKMLQKLPDLSQISGSPGEVVSITKGVGAIAQTGEGLLLLREVQLPGKRPQSGWDFVN
GTRLTVGEVLGNGS
>Mature_334_residues
MKIVFFGTPEFAVPTLEKLLLNQEFEVLAVVTQPDKRRERGNKLTPSPVKNMAIAHDLPVWQPERIKKDTETLNKLKQLD
ADAFVVVAYGQILSQKILDMPKLGCVNVHGSILPQYRGAAPIQWCLYNGETETGITTMLMDAGMDTGAMLLKATTPIGLL
DNADDVAQRLSVIGGDLLIETLYKLQQQEIQPIPQDNAAATYASLIQKQDYGLDWSRSALQLHNQIRGFYPNCTTTFRNQ
PLKITASFPLGAAYNDELPPELQKMLQKLPDLSQISGSPGEVVSITKGVGAIAQTGEGLLLLREVQLPGKRPQSGWDFVN
GTRLTVGEVLGNGS

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=246, Percent_Identity=29.6747967479675, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI21614513, Length=267, Percent_Identity=26.2172284644195, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI164663775, Length=331, Percent_Identity=26.2839879154079, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789683, Length=325, Percent_Identity=39.3846153846154, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788589, Length=250, Percent_Identity=30, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI133930964, Length=241, Percent_Identity=29.4605809128631, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550868, Length=328, Percent_Identity=27.4390243902439, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571984, Length=248, Percent_Identity=27.8225806451613, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585660, Length=230, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 36623; Mature: 36623

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFFGTPEFAVPTLEKLLLNQEFEVLAVVTQPDKRRERGNKLTPSPVKNMAIAHDLPV
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCCC
WQPERIKKDTETLNKLKQLDADAFVVVAYGQILSQKILDMPKLGCVNVHGSILPQYRGAA
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PIQWCLYNGETETGITTMLMDAGMDTGAMLLKATTPIGLLDNADDVAQRLSVIGGDLLIE
CEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TLYKLQQQEIQPIPQDNAAATYASLIQKQDYGLDWSRSALQLHNQIRGFYPNCTTTFRNQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCC
PLKITASFPLGAAYNDELPPELQKMLQKLPDLSQISGSPGEVVSITKGVGAIAQTGEGLL
CEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCHHHHCCCCEE
LLREVQLPGKRPQSGWDFVNGTRLTVGEVLGNGS
EEEEECCCCCCCCCCCCCCCCCEEEHHHHHCCCC
>Mature Secondary Structure
MKIVFFGTPEFAVPTLEKLLLNQEFEVLAVVTQPDKRRERGNKLTPSPVKNMAIAHDLPV
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHHCCCCC
WQPERIKKDTETLNKLKQLDADAFVVVAYGQILSQKILDMPKLGCVNVHGSILPQYRGAA
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
PIQWCLYNGETETGITTMLMDAGMDTGAMLLKATTPIGLLDNADDVAQRLSVIGGDLLIE
CEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
TLYKLQQQEIQPIPQDNAAATYASLIQKQDYGLDWSRSALQLHNQIRGFYPNCTTTFRNQ
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCC
PLKITASFPLGAAYNDELPPELQKMLQKLPDLSQISGSPGEVVSITKGVGAIAQTGEGLL
CEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCHHHHCCCCEE
LLREVQLPGKRPQSGWDFVNGTRLTVGEVLGNGS
EEEEECCCCCCCCCCCCCCCCCEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]