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The map label for this gene is cobA [H]

Identifier: 75909680

GI number: 75909680

Start: 4307191

End: 4308744

Strand: Direct

Name: cobA [H]

Synonym: Ava_3474

Alternate gene names: 75909680

Gene position: 4307191-4308744 (Clockwise)

Preceding gene: 75909679

Following gene: 75909681

Centisome position: 67.66

GC content: 42.86

Gene sequence:

>1554_bases
ATGACTCAACAAAATGGCAAAGTCTACCTTGTGGGTGCGGGGCCGGGAGATGTGGCATACCTCACAGTGAAAGCTTATAA
TCTACTGGCTACGGCTCAAGTGTTGGTTTATGATGCCCTAGTTGATGAGCAATTGTTGCAGTGTGTATCACCCGATTGTC
TTAAACTAGATGTAGGTAAGCGTGGCGGTAAACCCAGCACACCACAAGCCGATATCAATCATTTACTAGTCAAATACTGC
CAAGAAGGTGCATTAGTCGTTAGGTTGAAATCAGGCGACCCGTTTATTTTTGGACGCTGTACCTCTGAAATTGAGGCTTT
GAAAGCAGCAGGCTGTAAATTTGAGGTAGTACCAGGAATTTCCTCATCCATAGCCGCGCCTTTATTAGCAGGAATCCCTC
TCACTGACCCGGTGATGAGTCGTTGCTTTGCAGTGTTGACAGCCCACGAACCAGAAGTTTTAGACTGGGAGGCGCTGTCA
AGGTTAGACACCCTGGTCATACTAATGGGTGGGAAAAACTTAGCAGATATAATTAATGAACTTTTAAGAAGAGGCCAATC
GCATCTCACACCCATAGCTATTATTCGCTGGGCAGGAACCCCCAGTCAACAAATCTGGACTGGTCAACTGGGCGATATCC
TTGAACAAACAAGGGGTTTATCTCTTTCCCCAGCAGTCATCGTCATTGGTGAAGTTGTCGGACTACGCAAGTACCTACAA
CCTGAGAAAATATTTCCAGAGAACTCAACTACACCTATGTCCAGCAACCAACCTCTCACTGGAAAAACCATCCTCGTCAC
CCGTTCATCTGGTCAATCGAGTCAATTTAGCGATCGCCTAACTACACTTGGCGCTACAGTAATAGAAATGCCAGCATTAG
AAATAGGCTCCCCTTCTAGTTGGGAGGAATTAGATCAGGCGATCGCTAATTTATCTCAATTCGAGTGGTTAATTCTCACT
TCTACCAACGGTGTAGATTATTTCTTTGAAAGACTCAACCTCCAAGGTAAAGATACTCGTGCTTTAGCTGGGGTAAAAAT
TGCCGTTGTTGGGGAAAAAACCTCCCAAAGCCTCAAACAAAGAGGAATCCAAGCAGATTTTATCCCCCCTAACTTTGTAG
CTGATTCTTTAGTAGAGCATTTCCCTGAATCACTACTAAATAAAAAGATTTTATTTCCCAGAGTTGAAAGCGGTGGTAGA
GAAATTTTAGTCAAAGAACTATCAGCAAAAGGCGCAGAAGTAATTGAAGTAGCTGCTTATCAATCTTGTTGTCCTAGTAG
TATTCCCCCAGCAGCAGAACTAGCCTTAAAAAATCACACAATAGATATCATCACCTTTGCCAGTTCTAAAACTGTGCAAT
TTTTCCATCAACTTGTAGACAACATATTTCCTCACAATATCCCCAACACTTTAGCAGGAATATGTATTGCTTCCATCGGC
CCCCAAACCTCGAAAACTTGTCATACCCTATTAGGTCGAGTAGATGTAGAAGCTGAAGAATATACTTTAGATGGATTAAC
CCAATCCATCATAAATTGGGCATTAAAAGTTTAA

Upstream 100 bases:

>100_bases
AACGGGTTGCTAAATTATTAGAGCAAATGAATACTAAGACAATCTGATGAATGTGACTGATGTCTACGACGATGCTACAC
CTATGCACTAATCTAAAATC

Downstream 100 bases:

>100_bases
AATCCTCACCAATGACCTAAATATCCTCCGCGTTACTCCGCGCCTACCTCCCATCCCTCCGCGTTTAAAAAAGTTACGAA
TTGTCACTCCAAACCACATT

Product: uroporphyrinogen-III synthase / uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSPDCLKLDVGKRGGKPSTPQADINHLLVKYC
QEGALVVRLKSGDPFIFGRCTSEIEALKAAGCKFEVVPGISSSIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALS
RLDTLVILMGGKNLADIINELLRRGQSHLTPIAIIRWAGTPSQQIWTGQLGDILEQTRGLSLSPAVIVIGEVVGLRKYLQ
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGSPSSWEELDQAIANLSQFEWLILT
STNGVDYFFERLNLQGKDTRALAGVKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGR
EILVKELSAKGAEVIEVAAYQSCCPSSIPPAAELALKNHTIDIITFASSKTVQFFHQLVDNIFPHNIPNTLAGICIASIG
PQTSKTCHTLLGRVDVEAEEYTLDGLTQSIINWALKV

Sequences:

>Translated_517_residues
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSPDCLKLDVGKRGGKPSTPQADINHLLVKYC
QEGALVVRLKSGDPFIFGRCTSEIEALKAAGCKFEVVPGISSSIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALS
RLDTLVILMGGKNLADIINELLRRGQSHLTPIAIIRWAGTPSQQIWTGQLGDILEQTRGLSLSPAVIVIGEVVGLRKYLQ
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGSPSSWEELDQAIANLSQFEWLILT
STNGVDYFFERLNLQGKDTRALAGVKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGR
EILVKELSAKGAEVIEVAAYQSCCPSSIPPAAELALKNHTIDIITFASSKTVQFFHQLVDNIFPHNIPNTLAGICIASIG
PQTSKTCHTLLGRVDVEAEEYTLDGLTQSIINWALKV
>Mature_516_residues
TQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSPDCLKLDVGKRGGKPSTPQADINHLLVKYCQ
EGALVVRLKSGDPFIFGRCTSEIEALKAAGCKFEVVPGISSSIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSR
LDTLVILMGGKNLADIINELLRRGQSHLTPIAIIRWAGTPSQQIWTGQLGDILEQTRGLSLSPAVIVIGEVVGLRKYLQP
EKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGSPSSWEELDQAIANLSQFEWLILTS
TNGVDYFFERLNLQGKDTRALAGVKIAVVGEKTSQSLKQRGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGRE
ILVKELSAKGAEVIEVAAYQSCCPSSIPPAAELALKNHTIDIITFASSKTVQFFHQLVDNIFPHNIPNTLAGICIASIGP
QTSKTCHTLLGRVDVEAEEYTLDGLTQSIINWALKV

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=40.9090909090909, Blast_Score=165, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6322922, Length=237, Percent_Identity=32.0675105485232, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 55925; Mature: 55794

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSPDCLKLDVGK
CCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
RGGKPSTPQADINHLLVKYCQEGALVVRLKSGDPFIFGRCTSEIEALKAAGCKFEVVPGI
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHCCCEEEECCCC
SSSIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSRLDTLVILMGGKNLADIINE
CHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHH
LLRRGQSHLTPIAIIRWAGTPSQQIWTGQLGDILEQTRGLSLSPAVIVIGEVVGLRKYLQ
HHHCCCCCCCEEEEEEECCCCCCCEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGSPSS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
WEELDQAIANLSQFEWLILTSTNGVDYFFERLNLQGKDTRALAGVKIAVVGEKTSQSLKQ
HHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCCEECCEEEEEECCHHHHHHHH
RGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGREILVKELSAKGAEVIEVAAY
CCCEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHCCCCCHHHHHHHH
QSCCPSSIPPAAELALKNHTIDIITFASSKTVQFFHQLVDNIFPHNIPNTLAGICIASIG
HHCCCCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
PQTSKTCHTLLGRVDVEAEEYTLDGLTQSIINWALKV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQNGKVYLVGAGPGDVAYLTVKAYNLLATAQVLVYDALVDEQLLQCVSPDCLKLDVGK
CCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
RGGKPSTPQADINHLLVKYCQEGALVVRLKSGDPFIFGRCTSEIEALKAAGCKFEVVPGI
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHCCCEEEECCCC
SSSIAAPLLAGIPLTDPVMSRCFAVLTAHEPEVLDWEALSRLDTLVILMGGKNLADIINE
CHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCEEEEEECCCCHHHHHHH
LLRRGQSHLTPIAIIRWAGTPSQQIWTGQLGDILEQTRGLSLSPAVIVIGEVVGLRKYLQ
HHHCCCCCCCEEEEEEECCCCCCCEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PEKIFPENSTTPMSSNQPLTGKTILVTRSSGQSSQFSDRLTTLGATVIEMPALEIGSPSS
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
WEELDQAIANLSQFEWLILTSTNGVDYFFERLNLQGKDTRALAGVKIAVVGEKTSQSLKQ
HHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCCCCCEECCEEEEEECCHHHHHHHH
RGIQADFIPPNFVADSLVEHFPESLLNKKILFPRVESGGREILVKELSAKGAEVIEVAAY
CCCEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHCCCCCHHHHHHHH
QSCCPSSIPPAAELALKNHTIDIITFASSKTVQFFHQLVDNIFPHNIPNTLAGICIASIG
HHCCCCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
PQTSKTCHTLLGRVDVEAEEYTLDGLTQSIINWALKV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]