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The map label for this gene is pdhC [H]

Identifier: 75909383

GI number: 75909383

Start: 3943406

End: 3944704

Strand: Direct

Name: pdhC [H]

Synonym: Ava_3176

Alternate gene names: 75909383

Gene position: 3943406-3944704 (Clockwise)

Preceding gene: 75909381

Following gene: 75909384

Centisome position: 61.95

GC content: 48.58

Gene sequence:

>1299_bases
ATGAGCATTCACGAAATCTTCATGCCGGCGCTGAGTTCCACCATGACCGAAGGCAAAATTGTCTCCTGGGTGAAATCGCC
AGGCGATAAAGTGGAAAAAGGCGAAACAGTGGTGGTTGTCGAGTCAGACAAAGCAGATATGGATGTAGAAACCTTCTACG
AAGGGTATCTCGCTCACATCATTGTAGAAGCTGGTGATAGCGCACCTGTAGGAGCAGCGATCGCTTACGTAGCCGAAACC
GAAGCGGAAATCGAGGCCGCCAAGTCTTTAGGCAGTTCTGGTGGCGCTGCGGCCACTCCTAGCGCCCCTCCTGAACCAGT
CGCAGCCACCGCCGCCGTAGGTGTACCAGCCGCATCACAAAATGGCTCTAACCACCGTGAAGGAAGGCTTGTAGCTTCAC
CCCGCGCCCGCAAGTTGGCAAAAGAACTGAAAGTTGATTTGACCAGTCTCAAAGGTAGTGGCCCCTATGGTCGCATTGTG
GCCGATGACATAGAAGCTGCTGTTGGTAAAGTCAAGCAACCAGCCACTACACCGACTGCACCCACACCCACCTTTACCCC
AGCCCCAGTCCCAGCAACCAGGACTCCAGCACCAGCACCAGCACCAGCACCAGTAGCGGCCGCACCTGGGCAAGTAGTAC
CATTTAATACCCTACAAAACGCTGTCATTCGTAACATGGTGGCGAGTCTAGATGTACCCGTGTTCCGTGTGGGTTACACC
ATCACCACCGATGGCTTAGACAAACTTTACAAACAAATTAAATCCAAAGGTGTAACCATGACAGCGCTACTGGCGAAAGC
TGTAGCGGTGACATTACAAAAACACCCTTTATTAAATGCCAGCTATTCAGATCAAGGTCTTGTTTACCATCCTGACATCA
ATATTGCGGTAGCTGTAGCGATGGATGGTGGCGGTTTAATTACACCAGTATTAAAAAATGCCGACAAGATAGATATTTAC
TCTCTATCACGCACCTGGAAATCTCTAGTAGACAAGGCCAGAAGCAAACAACTACAACCAGATGAATACAGTGGTGGTAA
CTTTACCCTATCAAACTTGGGGATGTTTGGTGTAGATACATTTGATGCCATTTTACCCCCAGGACAAGGTTCAATTTTAG
CGATCGGCGCATCTCGTCCCCAATTGGTAGCCACAGGCGATGGTTCATTTGCTATCAAACAACAAATGCAGGTAAATATT
ACATCTGATCACCGGATTATTTACGGTGCTGATGCTGCCGCATTTTTACAAGATTTAGCCAAGTTAATTGAAACTGACGC
TCAATCTTTGACACTGTAA

Upstream 100 bases:

>100_bases
TTCCCAATTCCCAATTACTCAAAAACCCTCGTACCAAGATACAATTCGATCTGGCAAAAGCTGTTAAATTCCTTAAACCT
ATTGATTCTGAAACCACCCT

Downstream 100 bases:

>100_bases
AGGCGGTAGGGGTGTAAGGGTGAATTTCTGTGTATTCTCACCCCTACGCCCCATACCCCTATACCCCCAACCTCGGTTTA
TCATGGATCAGAGGATGATA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGYLAHIIVEAGDSAPVGAAIAYVAET
EAEIEAAKSLGSSGGAAATPSAPPEPVAATAAVGVPAASQNGSNHREGRLVASPRARKLAKELKVDLTSLKGSGPYGRIV
ADDIEAAVGKVKQPATTPTAPTPTFTPAPVPATRTPAPAPAPAPVAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYT
ITTDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINIAVAVAMDGGGLITPVLKNADKIDIY
SLSRTWKSLVDKARSKQLQPDEYSGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGSFAIKQQMQVNI
TSDHRIIYGADAAAFLQDLAKLIETDAQSLTL

Sequences:

>Translated_432_residues
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGYLAHIIVEAGDSAPVGAAIAYVAET
EAEIEAAKSLGSSGGAAATPSAPPEPVAATAAVGVPAASQNGSNHREGRLVASPRARKLAKELKVDLTSLKGSGPYGRIV
ADDIEAAVGKVKQPATTPTAPTPTFTPAPVPATRTPAPAPAPAPVAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYT
ITTDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINIAVAVAMDGGGLITPVLKNADKIDIY
SLSRTWKSLVDKARSKQLQPDEYSGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGSFAIKQQMQVNI
TSDHRIIYGADAAAFLQDLAKLIETDAQSLTL
>Mature_431_residues
SIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGYLAHIIVEAGDSAPVGAAIAYVAETE
AEIEAAKSLGSSGGAAATPSAPPEPVAATAAVGVPAASQNGSNHREGRLVASPRARKLAKELKVDLTSLKGSGPYGRIVA
DDIEAAVGKVKQPATTPTAPTPTFTPAPVPATRTPAPAPAPAPVAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYTI
TTDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINIAVAVAMDGGGLITPVLKNADKIDIYS
LSRTWKSLVDKARSKQLQPDEYSGGNFTLSNLGMFGVDTFDAILPPGQGSILAIGASRPQLVATGDGSFAIKQQMQVNIT
SDHRIIYGADAAAFLQDLAKLIETDAQSLTL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=446, Percent_Identity=33.6322869955157, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=32.4503311258278, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=32.4503311258278, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI110671329, Length=413, Percent_Identity=27.3607748184019, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=38.9221556886228, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI19923748, Length=171, Percent_Identity=36.2573099415205, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786946, Length=438, Percent_Identity=27.3972602739726, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786305, Length=418, Percent_Identity=28.9473684210526, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=30.7349665924276, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=25.6410256410256, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=211, Percent_Identity=33.6492890995261, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25146366, Length=165, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=31.1804008908686, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=26.6978922716628, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321632, Length=188, Percent_Identity=29.2553191489362, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129315, Length=444, Percent_Identity=31.3063063063063, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24582497, Length=437, Percent_Identity=30.4347826086957, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=27.6497695852535, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645909, Length=166, Percent_Identity=33.7349397590361, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44964; Mature: 44832

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGYLAHI
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEE
IVEAGDSAPVGAAIAYVAETEAEIEAAKSLGSSGGAAATPSAPPEPVAATAAVGVPAASQ
EEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
NGSNHREGRLVASPRARKLAKELKVDLTSLKGSGPYGRIVADDIEAAVGKVKQPATTPTA
CCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCC
PTPTFTPAPVPATRTPAPAPAPAPVAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEE
ITTDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINIAVAVA
EECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
MDGGGLITPVLKNADKIDIYSLSRTWKSLVDKARSKQLQPDEYSGGNFTLSNLGMFGVDT
ECCCCEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCH
FDAILPPGQGSILAIGASRPQLVATGDGSFAIKQQMQVNITSDHRIIYGADAAAFLQDLA
HHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEEEEEECCCEEEEECCHHHHHHHHH
KLIETDAQSLTL
HHHHCCHHHCCC
>Mature Secondary Structure 
SIHEIFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVETFYEGYLAHI
CHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHEEEE
IVEAGDSAPVGAAIAYVAETEAEIEAAKSLGSSGGAAATPSAPPEPVAATAAVGVPAASQ
EEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCC
NGSNHREGRLVASPRARKLAKELKVDLTSLKGSGPYGRIVADDIEAAVGKVKQPATTPTA
CCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCC
PTPTFTPAPVPATRTPAPAPAPAPVAAAPGQVVPFNTLQNAVIRNMVASLDVPVFRVGYT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHCCCCEEEEEEE
ITTDGLDKLYKQIKSKGVTMTALLAKAVAVTLQKHPLLNASYSDQGLVYHPDINIAVAVA
EECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEEEEE
MDGGGLITPVLKNADKIDIYSLSRTWKSLVDKARSKQLQPDEYSGGNFTLSNLGMFGVDT
ECCCCEEHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCH
FDAILPPGQGSILAIGASRPQLVATGDGSFAIKQQMQVNITSDHRIIYGADAAAFLQDLA
HHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEEEEEEEEECCCEEEEECCHHHHHHHHH
KLIETDAQSLTL
HHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]