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The map label for this gene is miaB

Identifier: 75909246

GI number: 75909246

Start: 3762009

End: 3763373

Strand: Direct

Name: miaB

Synonym: Ava_3037

Alternate gene names: 75909246

Gene position: 3762009-3763373 (Clockwise)

Preceding gene: 75909245

Following gene: 75909247

Centisome position: 59.1

GC content: 45.64

Gene sequence:

>1365_bases
ATGACCACTTCTAACCGCCGCTATCACATCACTACCTTTGGTTGCCAAATGAATAAAGCCGACTCAGAGCGCATGGCTGG
CATCTTAGAAGACATGGGCTTTGAGTTTTCCGAAGATCCCAACAATGCAGATGTGATTCTCTACAATACTTGTACAATTC
GGGATAATGCTGAACAGAAAGTCTATTCTTACCTCGGCAGACAAGCCAAGCGCAAACACGAACAACCAGACTTAACTTTA
GTTGTAGCTGGTTGTGTTGCCCAACAGGAAGGCGAAGCACTACTACGGCGAGTACCAGAACTAGATTTAGTCATGGGGCC
ACAACACGCCAACCGTCTCAAAGATTTGCTGGAGTCGGTGTTTGCGGGTAATCAAGTCGTTGCTACTGAAGCCGTCCATA
TTATGGAAGATATCACCCAGGCGCGGCGAGATAGCACCGTGACAGCTTGGGTAAATGTGATTTATGGCTGCAATGAACGC
TGTACTTATTGTGTAGTTCCCAACGTGCGCGGTGTGGAACAGTCTCGTACACCGGAAGCCGTTCGTGCAGAAATGGAAGA
ATTAGGACGGCAAGGTTACAAAGAAATCACCCTACTTGGTCAAAATATTGACGCTTACGGTAGAGATTTACCGGGTGCGA
CACCAGAAGGACGACACCTACACACCTTTACAGACTTACTGTATTACGTCCATGATGTGCCAGGTATAGAAAGAATCAGA
TTTGCTACCAGCCATCCCCGTTATTTTACGGAGAGACTAATTAAAGCTTGTGCAGAGTTGCCCAAAGTTTGCGAACATTT
CCACATTCCTTTTCAATCTGGCGATAACCAACTATTGAAAGCGATGGCACGGGGTTACACTCAGGAGAAATATCGCCGGA
TTATCGACACAATTCGTAGATATATGCCAGATGCTTCCATTAGTGCTGATGCAATTGTTGGGTTCCCAGGAGAGACAGAG
GAACAGTTTGAGAATACTTTGAAACTGGTGGATGATATTGGCTTTGACCAATTAAACACAGCCGCCTATTCACCCCGTCC
CGGCACACCAGCAGCTTTATGGGAAAATCAACTGAGTGAAGAAACCAAAAGCGATCGCCTACAAAGATTAAACCATTTAG
TGAACGTGAAAGCAGCCGAGCGATCGCAACGTTACATGGGGCGCATCGAGGAAGTTTTAGTAGAAGAACAAAACCCCAAA
GATCAAACCCAAGTCATGGGACGCACACGCGGTAATCGTCTCACCTTCTTCAAGGGAGATATTAATGAACTAAAAGGGCA
ATTAGTCAAGGTGAAAATTAACGAAGTTCGCGCTTTTAGTTTGACTGGTGAACCGATAGAAATACGGCAAGCTGTACAAG
TTTAA

Upstream 100 bases:

>100_bases
TCACAGCTTGGGGGTTGTTAAAGTTGCTCGACAATCACTAGAGTAAATAGAGAAGAACACAAAGCATATTTAACATACAG
CTAACTAAGCATAATTGCCC

Downstream 100 bases:

>100_bases
ATGAATTTACACCACCCTGGAAGTAATTATTTGTGGAGTCTGAATCTAAATGTCAAACTTGTTGCAAAGAATCACAGTCA
ACCCTAAACAGTGTGGTGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTL
VVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPEAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIR
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETE
EQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEETKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPK
DQTQVMGRTRGNRLTFFKGDINELKGQLVKVKINEVRAFSLTGEPIEIRQAVQV

Sequences:

>Translated_454_residues
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTL
VVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPEAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIR
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETE
EQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEETKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPK
DQTQVMGRTRGNRLTFFKGDINELKGQLVKVKINEVRAFSLTGEPIEIRQAVQV
>Mature_453_residues
TTSNRRYHITTFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTLV
VAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERC
TYCVVPNVRGVEQSRTPEAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIRF
ATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETEE
QFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEETKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKD
QTQVMGRTRGNRLTFFKGDINELKGQLVKVKINEVRAFSLTGEPIEIRQAVQV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=32.1503131524008, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=32.1503131524008, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=28.159645232816, Blast_Score=175, Evalue=9e-44,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=40.2247191011236, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=25.9423503325942, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=33.0434782608696, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=388, Percent_Identity=26.0309278350515, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356207, Length=481, Percent_Identity=32.016632016632, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=28.9130434782609, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ANAVT (Q3M8N9)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323542.1
- ProteinModelPortal:   Q3M8N9
- SMR:   Q3M8N9
- STRING:   Q3M8N9
- GeneID:   3681155
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3037
- NMPDR:   fig|240292.3.peg.2260
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q3M8N9
- ProtClustDB:   PRK14333
- BioCyc:   AVAR240292:AVA_3037-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51629; Mature: 51498

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEA
HCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
VRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIR
HHHHHHHHHHCCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRR
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
YMPDASISADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
ETKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTRGNRLTFFKGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECC
INELKGQLVKVKINEVRAFSLTGEPIEIRQAVQV
HHHHCCEEEEEEEEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
TTSNRRYHITTFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQK
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEA
HCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHH
VRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIR
HHHHHHHHHHCCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRR
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
YMPDASISADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
ETKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTRGNRLTFFKGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECC
INELKGQLVKVKINEVRAFSLTGEPIEIRQAVQV
HHHHCCEEEEEEEEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA