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The map label for this gene is lipA2 [H]

Identifier: 75909215

GI number: 75909215

Start: 3729775

End: 3730692

Strand: Direct

Name: lipA2 [H]

Synonym: Ava_3006

Alternate gene names: 75909215

Gene position: 3729775-3730692 (Clockwise)

Preceding gene: 75909211

Following gene: 75909216

Centisome position: 58.59

GC content: 48.8

Gene sequence:

>918_bases
ATGACTGCCTCACAACCTGCTCAGTTTAAGTCGGAAATCACGGCAATGCCCAGTTGGTTGCGCCGTCCCATTGGCAAAGC
TAGTGAACTGTCAACAGTACAGCGCATTATTAAACAGCGTCAAATTCATACAATTTGTGAAGAAGGTCGCTGCCCGAATC
GGGGTGAGTGCTATGCCCAAAAAACGGCTACATTCTTACTTATGGGGCCTACCTGCACCCGTGCTTGTGCTTTTTGTCAA
GTCGATAAGGGTCATGCACCAATGCCAATTGACCCAGAGGAAGCAGCAAAGGTGGCAGAAGCGGTACAACTTTTGGGACT
GCGTTATGTAGTACTTACTTCTGTCGCTCGTGATGATTTACAAGATCAGGGAGCAAGCCACTTTGTCCAAACTATGGAGG
CAATTCGTCAACTAAATCCGGGTACTCAAATTGAAGTGTTGACACCGGATTTTTGGGGTGGTGCAGGCGCAGGGGAAGCC
GGACAACGCCAGCGCCTAAAAATGATTGTAGAGGCGCAACCAGCTTGTTTTAACCATAACATTGAGACAGTGCGTCGCTT
AACCGGGCCAGTCCGCCGGGGTGCTAAATACGATCGCTCTTTGCGGGTTCTCTCTCTTGTCAAAGAACTAAACCCCCATA
TTCCCACCAAATCAGGCTTAATGGTGGGACATGGAGAGACGGTTACTGAACTAGTCGAAGCAATGACAGACTTAAGGGCT
ATAGGATGCGATCGCCTCACTATCGGTCAGTATATGCGCCCATCCCTAGAACATTTACCAGTCCAAAAATATTGGACACC
AGAAGAATTTGAGCATCTGGGCAAACTAGCCAGAGAAATAGGCTTTAGCCACGTCCGTTCGGGTCCACTAGTTCGCAGTT
CCTATCATGCTGGGGAGGAACCAATACCGTTCGGATAA

Upstream 100 bases:

>100_bases
TATCTCCCTACACCCCTAAACCCCTACACCCCCACACCCAGTCTCAACCGACAATTTTAGTGCGTAAGTCCAATAGAGAT
TTATTTTTATTTATCAGCCT

Downstream 100 bases:

>100_bases
GCAGGGGAGGCAGAGGAAGCAGAGGAGGCAGGGGAGGAAAGAAGTTTATTTTCATAATTTTTCTCTTCCCCTACTTCTGT
TCTCCCCTTACTTCCCCTGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTASQPAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRACAFCQ
VDKGHAPMPIDPEEAAKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGEA
GQRQRLKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPHIPTKSGLMVGHGETVTELVEAMTDLRA
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREIGFSHVRSGPLVRSSYHAGEEPIPFG

Sequences:

>Translated_305_residues
MTASQPAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRACAFCQ
VDKGHAPMPIDPEEAAKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGEA
GQRQRLKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPHIPTKSGLMVGHGETVTELVEAMTDLRA
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREIGFSHVRSGPLVRSSYHAGEEPIPFG
>Mature_304_residues
TASQPAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRACAFCQV
DKGHAPMPIDPEEAAKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGEAG
QRQRLKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPHIPTKSGLMVGHGETVTELVEAMTDLRAI
GCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREIGFSHVRSGPLVRSSYHAGEEPIPFG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=42.5675675675676, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI37577164, Length=259, Percent_Identity=40.5405405405405, Blast_Score=194, Evalue=9e-50,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=44.6808510638298, Blast_Score=257, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=293, Percent_Identity=43.0034129692833, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=301, Percent_Identity=42.5249169435216, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=42.7586206896552, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33836; Mature: 33705

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASQPAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQ
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KTATFLLMGPTCTRACAFCQVDKGHAPMPIDPEEAAKVAEAVQLLGLRYVVLTSVARDDL
CCEEEEEECCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGEAGQRQRLKMIVEAQPACFNHN
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCC
IETVRRLTGPVRRGAKYDRSLRVLSLVKELNPHIPTKSGLMVGHGETVTELVEAMTDLRA
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREIGFSHVRSGPLVRSSYHAGEE
HCCCCCHHHHHHCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCC
PIPFG
CCCCC
>Mature Secondary Structure 
TASQPAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQ
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KTATFLLMGPTCTRACAFCQVDKGHAPMPIDPEEAAKVAEAVQLLGLRYVVLTSVARDDL
CCEEEEEECCHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGEAGQRQRLKMIVEAQPACFNHN
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCC
IETVRRLTGPVRRGAKYDRSLRVLSLVKELNPHIPTKSGLMVGHGETVTELVEAMTDLRA
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREIGFSHVRSGPLVRSSYHAGEE
HCCCCCHHHHHHCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCC
PIPFG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]