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The map label for this gene is ydjE [H]

Identifier: 75909129

GI number: 75909129

Start: 3624841

End: 3625818

Strand: Direct

Name: ydjE [H]

Synonym: Ava_2919

Alternate gene names: 75909129

Gene position: 3624841-3625818 (Clockwise)

Preceding gene: 75909128

Following gene: 75909130

Centisome position: 56.94

GC content: 45.4

Gene sequence:

>978_bases
ATGAGTAATCCCCGTGTTTTGTGCCTCGGCGAAGTTCTATTCGATTGTTTAGCTGATCAATTAGGGCTAAAGCTGGAAGA
GGTAAAGTCTTGGACTGCTTATCCAGGAGGAGCGCCTGCTAATGTGGCTTGTGCTTTGGTGAAGTTGGGAACTCCCGCCG
GATTTATTGGTGCTGTCGGTGAGGATGAGCCAGGAAATGCGCTGGTTAAGCTATTACAAGAAGTAGGTGTAGATACAACG
GGTGTACAACGTCATTCTACTGCCCCAACAAGACAAGTCTATGTGGTGAGGGATCTAGCAGGCGATCGCACTTTCGCTGG
CTTCGGTCAGTATGATACCTCTGAATTTGCTGATACGCATTTGCAAGCCAAGCAATTACCAGAGTCAGTGTTTCAAGAAG
CAGATTTCCTGATTTTGGGTACTTTGGAATTAGCCTATCCCGATAGTGAAAAAGCTATTCACCGAGCTTTGGAACTAGCT
GAACAGTACGACCTGAAGATTGTGCTGGATGTTAACTGGCGGCCAGTATTTTGGCATGATGAAAATATTGCTAGGCAAAA
AATTCAGGAAATTTTCAAACGCGTTGATTTTCTCAAGCTTGCCAAAGAAGAAGCTGAATGGTTATTCGACACCTCTGATC
CAGGCGCTATTACTTACCGTTTAGGCTCCATTGAGGGGGTATTAGTGACTGATGGCGAAAATGGCTGTGGTTATTGCTTA
GGTGAGAATGAAGGTAAAATACCTGCTTTTGCTGTCTCTGTAGTAGATACCACGGGTGCAGGAGATAGCTTTCTGGCAGG
GTTTATCCATCAACTGAGTCAACATGGCATTCACAACTTGAATGATGCAGAGACAGCAAAGCGCATTATTACTTATGCTA
GTGCTGTAGGGGCGTTGACTACTACTAAAGCAGGTGCGATCGCTTCTCAACCCACTGCGGTGGAAGTGGAAACTTTTCTA
GCCTCCCATAAAATCTAG

Upstream 100 bases:

>100_bases
TTGCGCGGAGCGTGAATGCTAATAGATCAATATGAGATCACTAATCTAAAGCCAAAAAACTAAAATGAGATGAGCAATCC
CCGTGAAAAACTAAAATGAA

Downstream 100 bases:

>100_bases
CTGAACTACCCACATCTGACCCGTCGAGTCAGATGTGGGGCTTCCGTCGGTTCAAGCTAAAGTAGTAATAGTGATAGTTG
TAGTACTCAATTTGGCTGCT

Product: PfkB protein

Products: ADP; D-fructose 6-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSNPRVLCLGEVLFDCLADQLGLKLEEVKSWTAYPGGAPANVACALVKLGTPAGFIGAVGEDEPGNALVKLLQEVGVDTT
GVQRHSTAPTRQVYVVRDLAGDRTFAGFGQYDTSEFADTHLQAKQLPESVFQEADFLILGTLELAYPDSEKAIHRALELA
EQYDLKIVLDVNWRPVFWHDENIARQKIQEIFKRVDFLKLAKEEAEWLFDTSDPGAITYRLGSIEGVLVTDGENGCGYCL
GENEGKIPAFAVSVVDTTGAGDSFLAGFIHQLSQHGIHNLNDAETAKRIITYASAVGALTTTKAGAIASQPTAVEVETFL
ASHKI

Sequences:

>Translated_325_residues
MSNPRVLCLGEVLFDCLADQLGLKLEEVKSWTAYPGGAPANVACALVKLGTPAGFIGAVGEDEPGNALVKLLQEVGVDTT
GVQRHSTAPTRQVYVVRDLAGDRTFAGFGQYDTSEFADTHLQAKQLPESVFQEADFLILGTLELAYPDSEKAIHRALELA
EQYDLKIVLDVNWRPVFWHDENIARQKIQEIFKRVDFLKLAKEEAEWLFDTSDPGAITYRLGSIEGVLVTDGENGCGYCL
GENEGKIPAFAVSVVDTTGAGDSFLAGFIHQLSQHGIHNLNDAETAKRIITYASAVGALTTTKAGAIASQPTAVEVETFL
ASHKI
>Mature_324_residues
SNPRVLCLGEVLFDCLADQLGLKLEEVKSWTAYPGGAPANVACALVKLGTPAGFIGAVGEDEPGNALVKLLQEVGVDTTG
VQRHSTAPTRQVYVVRDLAGDRTFAGFGQYDTSEFADTHLQAKQLPESVFQEADFLILGTLELAYPDSEKAIHRALELAE
QYDLKIVLDVNWRPVFWHDENIARQKIQEIFKRVDFLKLAKEEAEWLFDTSDPGAITYRLGSIEGVLVTDGENGCGYCLG
ENEGKIPAFAVSVVDTTGAGDSFLAGFIHQLSQHGIHNLNDAETAKRIITYASAVGALTTTKAGAIASQPTAVEVETFLA
SHKI

Specific function: Unknown

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI87082343, Length=325, Percent_Identity=28.9230769230769, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082281, Length=247, Percent_Identity=27.5303643724696, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081971, Length=332, Percent_Identity=23.4939759036145, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.4

Molecular weight: Translated: 35140; Mature: 35009

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPRVLCLGEVLFDCLADQLGLKLEEVKSWTAYPGGAPANVACALVKLGTPAGFIGAVG
CCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEEEEECCCCCCEEECCC
EDEPGNALVKLLQEVGVDTTGVQRHSTAPTRQVYVVRDLAGDRTFAGFGQYDTSEFADTH
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHH
LQAKQLPESVFQEADFLILGTLELAYPDSEKAIHRALELAEQYDLKIVLDVNWRPVFWHD
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECC
ENIARQKIQEIFKRVDFLKLAKEEAEWLFDTSDPGAITYRLGSIEGVLVTDGENGCGYCL
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCEEEEEEECCCCCCEEEE
GENEGKIPAFAVSVVDTTGAGDSFLAGFIHQLSQHGIHNLNDAETAKRIITYASAVGALT
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TTKAGAIASQPTAVEVETFLASHKI
HCCCCCCCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure 
SNPRVLCLGEVLFDCLADQLGLKLEEVKSWTAYPGGAPANVACALVKLGTPAGFIGAVG
CCCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEEEEECCCCCCEEECCC
EDEPGNALVKLLQEVGVDTTGVQRHSTAPTRQVYVVRDLAGDRTFAGFGQYDTSEFADTH
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCCHHHHHHH
LQAKQLPESVFQEADFLILGTLELAYPDSEKAIHRALELAEQYDLKIVLDVNWRPVFWHD
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCEEEEECC
ENIARQKIQEIFKRVDFLKLAKEEAEWLFDTSDPGAITYRLGSIEGVLVTDGENGCGYCL
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCEEEEEEECCCCCCEEEE
GENEGKIPAFAVSVVDTTGAGDSFLAGFIHQLSQHGIHNLNDAETAKRIITYASAVGALT
CCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TTKAGAIASQPTAVEVETFLASHKI
HCCCCCCCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose

Specific reaction: ATP + D-fructose = ADP + D-fructose 6-phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]