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The map label for this gene is gcvH

Identifier: 75908973

GI number: 75908973

Start: 3402866

End: 3403258

Strand: Direct

Name: gcvH

Synonym: Ava_2761

Alternate gene names: 75908973

Gene position: 3402866-3403258 (Clockwise)

Preceding gene: 75908972

Following gene: 75908974

Centisome position: 53.46

GC content: 42.24

Gene sequence:

>393_bases
ATGTCGTCGTTTGAATACCCTCAAGATTTAAGATATTTGGATACTCATGAATATGTGAGGTTAGATGGCGAAATCGCTAC
TATCGGTATTACAGAATTTGCTGTAGATCAATTGGGTGATGTGGTTTTTCTAGAACTGCCAGATATTGGCGATTTACTGA
CTAAAGGAGACACTTTCGGCACAATTGAATCAGTCAAAGCCGTCGAAGACTTGAACGCACCAATCACAGGAACAGTTGTA
GAACGCAACGAAATTTTGATTGAATCTCCCGACGCAGTTGCAGATGATCCCTACGGTGAAGGATGGTTCTTGAAAGTACG
TGTGAATGACCCTGACGAAGTAAATGATGCTTTGACGGCAGATGAATATCGTGCTGAGGTAGAAGGGGAATAG

Upstream 100 bases:

>100_bases
TGAATGTGTTGTAAGATACTCAATCACAGTATGGTCATTAACTAAATATGGTCTTAATTACTTTGGGACGCTAATTGTTT
AATATTTCAGAGGAAAAGAA

Downstream 100 bases:

>100_bases
TTAGGGGTTTAGGGGTATGGGGGTATAAATATTTGAAACCCGTATACCCGTACATCCTTACACCCTTACACCCGGTCTCA
ATAGAAAATCTGGATGCGTA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MSSFEYPQDLRYLDTHEYVRLDGEIATIGITEFAVDQLGDVVFLELPDIGDLLTKGDTFGTIESVKAVEDLNAPITGTVV
ERNEILIESPDAVADDPYGEGWFLKVRVNDPDEVNDALTADEYRAEVEGE

Sequences:

>Translated_130_residues
MSSFEYPQDLRYLDTHEYVRLDGEIATIGITEFAVDQLGDVVFLELPDIGDLLTKGDTFGTIESVKAVEDLNAPITGTVV
ERNEILIESPDAVADDPYGEGWFLKVRVNDPDEVNDALTADEYRAEVEGE
>Mature_129_residues
SSFEYPQDLRYLDTHEYVRLDGEIATIGITEFAVDQLGDVVFLELPDIGDLLTKGDTFGTIESVKAVEDLNAPITGTVVE
RNEILIESPDAVADDPYGEGWFLKVRVNDPDEVNDALTADEYRAEVEGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=39.8230088495575, Blast_Score=102, Evalue=9e-23,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=38.9380530973451, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=40, Blast_Score=101, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=44.6428571428571, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=39.2857142857143, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=109, Percent_Identity=39.4495412844037, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=37.8151260504202, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_ANAVT (Q3M9G2)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323269.1
- ProteinModelPortal:   Q3M9G2
- SMR:   Q3M9G2
- STRING:   Q3M9G2
- GeneID:   3681889
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2761
- NMPDR:   fig|240292.3.peg.1163
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   AVAR240292:AVA_2761-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14413; Mature: 14282

Theoretical pI: Translated: 3.62; Mature: 3.62

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFEYPQDLRYLDTHEYVRLDGEIATIGITEFAVDQLGDVVFLELPDIGDLLTKGDTFG
CCCCCCCCCCEECCCCCEEEECCCEEEEEHHHHHHHHCCCEEEEECCCCHHHHHCCCCCC
TIESVKAVEDLNAPITGTVVERNEILIESPDAVADDPYGEGWFLKVRVNDPDEVNDALTA
CHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHCCCCC
DEYRAEVEGE
CCCEEEECCC
>Mature Secondary Structure 
SSFEYPQDLRYLDTHEYVRLDGEIATIGITEFAVDQLGDVVFLELPDIGDLLTKGDTFG
CCCCCCCCCEECCCCCEEEECCCEEEEEHHHHHHHHCCCEEEEECCCCHHHHHCCCCCC
TIESVKAVEDLNAPITGTVVERNEILIESPDAVADDPYGEGWFLKVRVNDPDEVNDALTA
CHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHCCCCC
DEYRAEVEGE
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA