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The map label for this gene is dacB [H]

Identifier: 75908868

GI number: 75908868

Start: 3285444

End: 3286763

Strand: Direct

Name: dacB [H]

Synonym: Ava_2656

Alternate gene names: 75908868

Gene position: 3285444-3286763 (Clockwise)

Preceding gene: 75908867

Following gene: 75908869

Centisome position: 51.61

GC content: 46.36

Gene sequence:

>1320_bases
ATGCTGGAATTGTTGAGTTCGGGTTTGGTTTCTATCTGGCTGGAAATGGCTGGTGTGCAAATCAAGCCTGCGGATGCTTT
AGATGCACTAACTTGGCAAAGTAGCCCAGGCTTGGTTATTGCACCTGATCCGAATCCAGCAGGAATTAATACAGTCAAGG
AATATCTGAAGGGATTAGTCACGACAAAATTAATTGCTCAGAATTTAGCAGAAAGCCAAGGAATTTGGTTGCAGTCAGGG
CCGATGTTGATGGCTAATCATCAAGGAACTACACCTCTGCCGGCAGCTTCTCTCACTAAAATTGCTACTTCACTGGCGGC
TTTTACTACTTTCGGCCCAGAACATCAATTTGAAACGACGATCGCCACTAATGCTTCAGTTGTTAATGGCGTATTACAAG
GTGATTTAATCATTAATGGCGGCGGCGACCCGATGTTTGTTTGGGAAGAAGCGATCGCCCTGGGTAATACGCTCAATAAA
ATGGGAATCAAGCAGGTAAAGGGCAATTTGGTAATTACAGGCAATTTTGCCATGAATTTCCAACGCCATCCCCTCTTGGC
TGGGCAAATGCTCAAACAATCACTAAATTCTAAAACCTGGACTCGTCCAGCAAATTATATACATTCCATCATGCCTAAGG
GAACACCAAAGCCCCAGGTAGTAATTAATGGCACAATCAAATTTTTCGCCCAGGCCAACCCCAAACAAACTTTACTTGTG
CGTCACCGTTCCTTACCCTTGAAACAATTGATCAAGGAGATGAACGTTTACAGCAATAATGAGATGGCGCAGATGTTGGC
AGAAGCGGTGGGAGGCCATACTGTAGTCCAAGCCAAAGCTGCCAAACTAGCAAGAGTACCAGAGACAGAAATTCAATTAA
TTAATGGTTCGGGACTGGGCCCAGAAAATCGAATTTCTCCCAGGGCGGTTTGTGCGATGTTGATGGCGCTGCAACATGAA
GCTGCTGCGGCTCAACTCAATCTAGCAGACTTGTTTCCCATCTCTGGCTTTGATCACCGAGGCACAATGCACTCTCGACA
TTTGCCGATGGCTACTGTGATGAAAACTGGGACGTTGCGGGATGTAAGTGCTTTAGCTGGAGTTGTACCCACACGCGATC
GCGGTTTGGTTTGGTTCGCTATCATTAACCGTGGCCCCCAAATTGGCGCTTTCCGCCAACAACAAGACAAACTTTTACAA
CGTCTAGTACAGCAATTACAAGTAGCCCCTAGCACACCCACAGCCCTTACCCCCCATTCACCTAAATCTTTACCCGATCT
GGGGAATACTAACCGCAGTGAAATTGTGTATGGTGGCTAG

Upstream 100 bases:

>100_bases
AGAAAATACCAGTTATGATGACTCAATGCTGATGAACACCATAAAGCGCGTATATTAGCACAGGAAACAATATATTACAG
TCATAAGTGGGGAGTGAATC

Downstream 100 bases:

>100_bases
TAGGGTAATTCGTAATTACGCTGTTGTGAGGGTGATAAACATTGCCGATAGGCGTGTATTTACACCGCAACTAGTATCAC
TACACGAAATTAAGCAGGTA

Product: peptidase S13, D-Ala-D-Ala carboxypeptidase C

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVVINGTIKFFAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHTVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAVCAMLMALQHE
AAAAQLNLADLFPISGFDHRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRDRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG

Sequences:

>Translated_439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVVINGTIKFFAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHTVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAVCAMLMALQHE
AAAAQLNLADLFPISGFDHRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRDRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG
>Mature_439_residues
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLVTTKLIAQNLAESQGIWLQSG
PMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETTIATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNK
MGIKQVKGNLVITGNFAMNFQRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVVINGTIKFFAQANPKQTLLV
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHTVVQAKAAKLARVPETEIQLINGSGLGPENRISPRAVCAMLMALQHE
AAAAQLNLADLFPISGFDHRGTMHSRHLPMATVMKTGTLRDVSALAGVVPTRDRGLVWFAIINRGPQIGAFRQQQDKLLQ
RLVQQLQVAPSTPTALTPHSPKSLPDLGNTNRSEIVYGG

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=98, Percent_Identity=36.734693877551, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47270; Mature: 47270

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLV
CHHHHHHHHHHHHHHHHCCEECCCHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHH
TTKLIAQNLAESQGIWLQSGPMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETT
HHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCE
IATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNKMGIKQVKGNLVITGNFAMNF
EECCHHHHHHHEECCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHCCCEEEEECEEECC
QRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVVINGTIKFFAQANPKQTLLV
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEE
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHTVVQAKAAKLARVPETEIQLINGSGLG
ECCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCC
PENRISPRAVCAMLMALQHEAAAAQLNLADLFPISGFDHRGTMHSRHLPMATVMKTGTLR
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCH
DVSALAGVVPTRDRGLVWFAIINRGPQIGAFRQQQDKLLQRLVQQLQVAPSTPTALTPHS
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKSLPDLGNTNRSEIVYGG
CCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MLELLSSGLVSIWLEMAGVQIKPADALDALTWQSSPGLVIAPDPNPAGINTVKEYLKGLV
CHHHHHHHHHHHHHHHHCCEECCCHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHHHHH
TTKLIAQNLAESQGIWLQSGPMLMANHQGTTPLPAASLTKIATSLAAFTTFGPEHQFETT
HHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCE
IATNASVVNGVLQGDLIINGGGDPMFVWEEAIALGNTLNKMGIKQVKGNLVITGNFAMNF
EECCHHHHHHHEECCEEEECCCCCEEHHHHHHHHCCHHHHHHHHHHCCCEEEEECEEECC
QRHPLLAGQMLKQSLNSKTWTRPANYIHSIMPKGTPKPQVVINGTIKFFAQANPKQTLLV
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECEEEEEECCCCCCEEEE
RHRSLPLKQLIKEMNVYSNNEMAQMLAEAVGGHTVVQAKAAKLARVPETEIQLINGSGLG
ECCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCC
PENRISPRAVCAMLMALQHEAAAAQLNLADLFPISGFDHRGTMHSRHLPMATVMKTGTLR
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCH
DVSALAGVVPTRDRGLVWFAIINRGPQIGAFRQQQDKLLQRLVQQLQVAPSTPTALTPHS
HHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKSLPDLGNTNRSEIVYGG
CCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]