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The map label for this gene is miaA [H]

Identifier: 75908732

GI number: 75908732

Start: 3113389

End: 3114273

Strand: Direct

Name: miaA [H]

Synonym: Ava_2518

Alternate gene names: 75908732

Gene position: 3113389-3114273 (Clockwise)

Preceding gene: 75908730

Following gene: 75908733

Centisome position: 48.91

GC content: 41.24

Gene sequence:

>885_bases
ATGACTAAATTAATCGTAATTTGTGGAGCTACGGCGACGGGTAAATCTGGTTTGGCTTTGAACTTAGCCATGCGGTTGGG
TTCTGTGATTTTGAGTGCTGATTCTCGTCAAGTGTATCGTGAATTTGATATTGGTACAGCGAAGCCAACGCTGGCAGAAC
AAAAAGCCGTTCCTCATTATCTAATAGATATCTGCACTCCCAGAGAAACGATGACAGTTGCAGATTATCAAGAACAGGCA
CAAGCACTAATTAATTCTCTGCCAGTTTCGCCGTTATTGTTAGTGGGAGGTACTGGTTTATATATACGTTCTATTGTGCA
GGGGATGAAGATCCCCAGGGTTGCGCCGAATTATGAATTGCGATCGCAACTCGAATCTTTAGGTCAAACTACACTCTACG
GCATATTACAACAAGTTGATCCCGTTGCGGCTCAAAAGATTCATCCCCATGATCCTGTACGGACTTTACGGGCAGTAGAA
GTATTTTACGTTACTGGCATTCCCATATCGGCACAGCAAGGAGAAAATCCCCCAGATTACCCTATTTTACAAATTGGATT
AGATTGTGAAATGGAAAGATTGAGTGAGCGCATTCACAAACGCACTGAACAAATGATAGCAGATGGCTTAGTTGGAGAAG
TTGAATATCTGTGCCAAAAATATGGTGCTGATTTACCCTTGTTAAATACTTTAGGATATCAAGAAATTAAGCAATATTTA
GCTGGAGAAATTTCCTTAGAAGCAGCCAAAGAATTAATCGTACTGCATACAAGACAATTTGCCAAACGCCAACGCACCTG
GTTTAGAGCCTATCCCCAAATTGAGTGGTTTAATGCAGATGATGCTGATTTATTAGATATTGTTTGGCAGCGCATACAAC
AGTAG

Upstream 100 bases:

>100_bases
CAAAATCTAAAAAGAGCAAAATGCTTAAGAATTTTAGCACAAAAGCGTTGATGGGAAGCAGGGGAGTAGGGGAAAACTAT
TGACAACTGATAACTGACCA

Downstream 100 bases:

>100_bases
AGAAATTTGATCAAATGCCTCTAAATGATACAGTTACAAATCCAAAGACCCTCTTAGTTATTTACCTACAAAAACTGAGT
AAGCTGTGCCAAAATCTACA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTKLIVICGATATGKSGLALNLAMRLGSVILSADSRQVYREFDIGTAKPTLAEQKAVPHYLIDICTPRETMTVADYQEQA
QALINSLPVSPLLLVGGTGLYIRSIVQGMKIPRVAPNYELRSQLESLGQTTLYGILQQVDPVAAQKIHPHDPVRTLRAVE
VFYVTGIPISAQQGENPPDYPILQIGLDCEMERLSERIHKRTEQMIADGLVGEVEYLCQKYGADLPLLNTLGYQEIKQYL
AGEISLEAAKELIVLHTRQFAKRQRTWFRAYPQIEWFNADDADLLDIVWQRIQQ

Sequences:

>Translated_294_residues
MTKLIVICGATATGKSGLALNLAMRLGSVILSADSRQVYREFDIGTAKPTLAEQKAVPHYLIDICTPRETMTVADYQEQA
QALINSLPVSPLLLVGGTGLYIRSIVQGMKIPRVAPNYELRSQLESLGQTTLYGILQQVDPVAAQKIHPHDPVRTLRAVE
VFYVTGIPISAQQGENPPDYPILQIGLDCEMERLSERIHKRTEQMIADGLVGEVEYLCQKYGADLPLLNTLGYQEIKQYL
AGEISLEAAKELIVLHTRQFAKRQRTWFRAYPQIEWFNADDADLLDIVWQRIQQ
>Mature_293_residues
TKLIVICGATATGKSGLALNLAMRLGSVILSADSRQVYREFDIGTAKPTLAEQKAVPHYLIDICTPRETMTVADYQEQAQ
ALINSLPVSPLLLVGGTGLYIRSIVQGMKIPRVAPNYELRSQLESLGQTTLYGILQQVDPVAAQKIHPHDPVRTLRAVEV
FYVTGIPISAQQGENPPDYPILQIGLDCEMERLSERIHKRTEQMIADGLVGEVEYLCQKYGADLPLLNTLGYQEIKQYLA
GEISLEAAKELIVLHTRQFAKRQRTWFRAYPQIEWFNADDADLLDIVWQRIQQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.5286624203822, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI1790613, Length=291, Percent_Identity=36.7697594501718, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=25.6578947368421, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=287, Percent_Identity=27.5261324041812, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24649830, Length=266, Percent_Identity=35.7142857142857, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 32948; Mature: 32816

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLIVICGATATGKSGLALNLAMRLGSVILSADSRQVYREFDIGTAKPTLAEQKAVPHY
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCCCHH
LIDICTPRETMTVADYQEQAQALINSLPVSPLLLVGGTGLYIRSIVQGMKIPRVAPNYEL
HHEECCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHH
RSQLESLGQTTLYGILQQVDPVAAQKIHPHDPVRTLRAVEVFYVTGIPISAQQGENPPDY
HHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHEEEEECCCEECCCCCCCCCC
PILQIGLDCEMERLSERIHKRTEQMIADGLVGEVEYLCQKYGADLPLLNTLGYQEIKQYL
CEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
AGEISLEAAKELIVLHTRQFAKRQRTWFRAYPQIEWFNADDADLLDIVWQRIQQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKLIVICGATATGKSGLALNLAMRLGSVILSADSRQVYREFDIGTAKPTLAEQKAVPHY
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHCCCHH
LIDICTPRETMTVADYQEQAQALINSLPVSPLLLVGGTGLYIRSIVQGMKIPRVAPNYEL
HHEECCCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCHHH
RSQLESLGQTTLYGILQQVDPVAAQKIHPHDPVRTLRAVEVFYVTGIPISAQQGENPPDY
HHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHEEEEECCCEECCCCCCCCCC
PILQIGLDCEMERLSERIHKRTEQMIADGLVGEVEYLCQKYGADLPLLNTLGYQEIKQYL
CEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHH
AGEISLEAAKELIVLHTRQFAKRQRTWFRAYPQIEWFNADDADLLDIVWQRIQQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]