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The map label for this gene is kojP [H]

Identifier: 75908496

GI number: 75908496

Start: 2834085

End: 2836364

Strand: Direct

Name: kojP [H]

Synonym: Ava_2279

Alternate gene names: 75908496

Gene position: 2834085-2836364 (Clockwise)

Preceding gene: 75908494

Following gene: 75908497

Centisome position: 44.52

GC content: 44.52

Gene sequence:

>2280_bases
ATGCTTGACGACGTAGATATATCTGTGGAAAATTCCCTTGAGCCACTGCTAGATGCTAACGAATGGAATGTGATTGAGAC
AGAGTTTAACCCTACACAACTACACCACAAAGAAACTGTCTTCACTCTCAGTAATGGTTATCTAGGAACCAGGGGAAGTT
TTGAGGAAAGTTATCCAGAGGATTTTGCCGCCTCAATGATTCATGGCATTTATGATGATACGCAAATTTCCCATACAGAA
TTAGTAAATTGTCCTAACTGGTTGCCATTGGTAGTGGAGGTGGCGGGTGAACAATTTGGCATGAATTCGGGGGAAATTCT
CCATTATGAGCGTCGGTTGGACTTGCGATTGGGTTTAGTTAGTCGTGATGTGCGTTGGCGTTCTCCCCAAGGTCATACTG
TAGATTTGCATTTTGAACGTTTTGCTAGTTTGGCAGATCAACACGTATTAGCGATTCGTTGTCAAATCACATCTGTAGAT
TTTACAGGTGAGATTACAGTCACAGCCACATTTGATGGGGAACCAAAAACCCAAGGGACTCCCCATTGGCAAACTTTAAA
CCAAGGTGGCGTAGACCAGATTCTTTGGTTACAAAGTCAAACCCTGCATTCTGGCCTTGGTTTGGGAATGAGTGCCAAGT
TAATCATAGATGGTGACGAGACTGCGGCTGTTTCTGTCAAAAATGCTGATTTTCCAACTTTGGGGACTACTTTTGAATGT
AACCCAGGAAAAACAGTCATTGTGGAAAAGATTGTGACCGTGTTTACTTCCAGAGAAACAGACATCCCCATTGCAGCCGC
ACTAGAAAGATTAGCTGATGAACCCAGATACACTACCCTATTAGCTGCCCATATTGCTGCTTGGGAGCAAGTTTGGCAAA
ATAGCGACATTATTATTGAAGGCGATCGCCAAGCCCAACTCAGTGTCCGTTACAATCTTTTTCAATTACTATCCGTAGCA
CCAAGCCACGACGACCATGTAAGTATAGCCCCTAAAACCCTCTCCGGCTTTGCCTATAGCGGACACATCTTTTGGGACAC
CGAAATATTTATCCTCCCCTTCCTCACATTTACCCAACCAGCCTTAGCGCGAAACTTACTCACCTACCGCTACCACACCT
TACCAGGCGCAAGACGCAAAGCCCAAGAAGCCGGTTATCAAGGCGCAATGTTTGCTTGGGAAAGTGCTACAACCGGTGAT
GAAGTCACCCCCCGGTGGGTTCCTGGTGCTAATGGTGAGTTAGTAAGAATTTGGTGCGGTGATATCGAAGTACATATCAC
AGCTGACGTAGCTTATGCAGTTTGGCACTACTGGCAAACTACCGACGATGATGAGTGGATGCACGACTATGGTGCAGAAA
TAATTCTTGATACAGCCGTGTTTTGGGAAAGTCGCGTCCAGTGGAATCAACAACGCCACAGTTACGACATTCTCGATGTG
ATTGGCCCCGATGAAAATCATGATCGCGTTGATAATAACGCCTTTACCAACTTGATGGTACAGTGGCACTTACAATCAGC
TTTAGCCCTGTGGGATTGGTTCAAACACGTTTATCCCGAAAAAGCTGCACAACTAGTAAAACAACTTAACTTAACTACAG
AACGTCTACACCGTTGGGCAGAAATTCAAGAACGTATCTTTGTCAATCAAGATATAACCACAGGTTTAATCGAACAGTTT
GAGGGCTTTTTTCAACTAGAAGATGTCAACCTAGCTGATTACGAACCCCGCACAAAATCTTTGCAAGGTTTGTTAGGGAT
TGAAGCCACTAGCCAAAAGCAAATACTCAAACAGCCTGATGTGTTGATGCTACTTTACTTGCTACGTCAAGCTTACGACT
ATAACACCCTCAAAGTTAACTGGGACTATTACACCCAACGCACCGACCATACTTATGGTTCTTCACTAGGCCCAGCAATT
CATGCTGTCTTAGCTTGCGACCTTAATAACCCTACAGAAGCCTACACTCATTTTTTAAGAGCTGCATTAGTAGATTTGGA
AGATGTCAGACTCAATGCCGCCGAAGGAATCCACGCCGCCAGTGCAGGAGGAGTTTGGCAAGCTGTTGTTTTAGGGTTCG
GGGGTGTGAGAATGACTCAATTTGGCCCGATGGCTTGTCCTAATCTCCCGTCAACATGGAAACGCTTGAAATTTAGGCTG
CAATGGCGTAATGAATGGTATGAGTTTGATTTACAACCAGAAACAGCAATGTCAGTAGTGACACAAGAAGCTTCAGTTCC
TGTTTTTGCCACTGTACAAGCTGTAATGGTTAATGGGTAA

Upstream 100 bases:

>100_bases
ACGTATCAACAATAAAAGCCAGGATTTAATTATCTATGAATCCTACCGATTATGTTTCATCCTGACAACAGCCATCTGCA
AATTTTAGGTTTTCTAACTC

Downstream 100 bases:

>100_bases
TGGGTAATGGGTAATGGGTAATTGGTTTGTCGCAATTACCCATTAGGATGTGGGATTGACCTTTTTTGAATTTTTAATTT
TGAACTGGAACGTAAAGCCT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 759; Mature: 759

Protein sequence:

>759_residues
MLDDVDISVENSLEPLLDANEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSFEESYPEDFAASMIHGIYDDTQISHTE
LVNCPNWLPLVVEVAGEQFGMNSGEILHYERRLDLRLGLVSRDVRWRSPQGHTVDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTATFDGEPKTQGTPHWQTLNQGGVDQILWLQSQTLHSGLGLGMSAKLIIDGDETAAVSVKNADFPTLGTTFEC
NPGKTVIVEKIVTVFTSRETDIPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PSHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPALARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELVRIWCGDIEVHITADVAYAVWHYWQTTDDDEWMHDYGAEIILDTAVFWESRVQWNQQRHSYDILDV
IGPDENHDRVDNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVKQLNLTTERLHRWAEIQERIFVNQDITTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPSTWKRLKFRL
QWRNEWYEFDLQPETAMSVVTQEASVPVFATVQAVMVNG

Sequences:

>Translated_759_residues
MLDDVDISVENSLEPLLDANEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSFEESYPEDFAASMIHGIYDDTQISHTE
LVNCPNWLPLVVEVAGEQFGMNSGEILHYERRLDLRLGLVSRDVRWRSPQGHTVDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTATFDGEPKTQGTPHWQTLNQGGVDQILWLQSQTLHSGLGLGMSAKLIIDGDETAAVSVKNADFPTLGTTFEC
NPGKTVIVEKIVTVFTSRETDIPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PSHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPALARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELVRIWCGDIEVHITADVAYAVWHYWQTTDDDEWMHDYGAEIILDTAVFWESRVQWNQQRHSYDILDV
IGPDENHDRVDNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVKQLNLTTERLHRWAEIQERIFVNQDITTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPSTWKRLKFRL
QWRNEWYEFDLQPETAMSVVTQEASVPVFATVQAVMVNG
>Mature_759_residues
MLDDVDISVENSLEPLLDANEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSFEESYPEDFAASMIHGIYDDTQISHTE
LVNCPNWLPLVVEVAGEQFGMNSGEILHYERRLDLRLGLVSRDVRWRSPQGHTVDLHFERFASLADQHVLAIRCQITSVD
FTGEITVTATFDGEPKTQGTPHWQTLNQGGVDQILWLQSQTLHSGLGLGMSAKLIIDGDETAAVSVKNADFPTLGTTFEC
NPGKTVIVEKIVTVFTSRETDIPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVA
PSHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQPALARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGD
EVTPRWVPGANGELVRIWCGDIEVHITADVAYAVWHYWQTTDDDEWMHDYGAEIILDTAVFWESRVQWNQQRHSYDILDV
IGPDENHDRVDNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVKQLNLTTERLHRWAEIQERIFVNQDITTGLIEQF
EGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAI
HAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPSTWKRLKFRL
QWRNEWYEFDLQPETAMSVVTQEASVPVFATVQAVMVNG

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=443, Percent_Identity=30.0225733634311, Blast_Score=158, Evalue=2e-38,
Organism=Escherichia coli, GI1787575, Length=713, Percent_Identity=30.9957924263675, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6325283, Length=551, Percent_Identity=22.6860254083485, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583760, Length=309, Percent_Identity=29.7734627831715, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 86004; Mature: 86004

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDVDISVENSLEPLLDANEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSFEESYPE
CCCCCEEECCCCCCHHCCCCCCCEEEECCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCH
DFAASMIHGIYDDTQISHTELVNCPNWLPLVVEVAGEQFGMNSGEILHYERRLDLRLGLV
HHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHEEEHHHHHCCCCCCEEEEEEECCEEEEEE
SRDVRWRSPQGHTVDLHFERFASLADQHVLAIRCQITSVDFTGEITVTATFDGEPKTQGT
ECCCEECCCCCCEEEEEHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCC
PHWQTLNQGGVDQILWLQSQTLHSGLGLGMSAKLIIDGDETAAVSVKNADFPTLGTTFEC
CCHHCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEC
NPGKTVIVEKIVTVFTSRETDIPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GDRQAQLSVRYNLFQLLSVAPSHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQP
CCCCEEEEEHHHHHHHHHCCCCCCCCEEECCCHHCCEEEECEEEECCCEEEEEHHHHCCH
ALARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGDEVTPRWVPGANGELVRIWCG
HHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DIEVHITADVAYAVWHYWQTTDDDEWMHDYGAEIILDTAVFWESRVQWNQQRHSYDILDV
CEEEEEEEHHHEEHHHHHCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEE
IGPDENHDRVDNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVKQLNLTTERLHRWA
CCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
EIQERIFVNQDITTGLIEQFEGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPD
HHHHHEECCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCC
VLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAIHAVLACDLNNPTEAYTHFLR
HHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHH
AALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPSTWKRLKFRL
HHHHCHHHHEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHEEEE
QWRNEWYEFDLQPETAMSVVTQEASVPVFATVQAVMVNG
EECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MLDDVDISVENSLEPLLDANEWNVIETEFNPTQLHHKETVFTLSNGYLGTRGSFEESYPE
CCCCCEEECCCCCCHHCCCCCCCEEEECCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCH
DFAASMIHGIYDDTQISHTELVNCPNWLPLVVEVAGEQFGMNSGEILHYERRLDLRLGLV
HHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHEEEHHHHHCCCCCCEEEEEEECCEEEEEE
SRDVRWRSPQGHTVDLHFERFASLADQHVLAIRCQITSVDFTGEITVTATFDGEPKTQGT
ECCCEECCCCCCEEEEEHHHHHHHHCCEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCC
PHWQTLNQGGVDQILWLQSQTLHSGLGLGMSAKLIIDGDETAAVSVKNADFPTLGTTFEC
CCHHCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEC
NPGKTVIVEKIVTVFTSRETDIPIAAALERLADEPRYTTLLAAHIAAWEQVWQNSDIIIE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEE
GDRQAQLSVRYNLFQLLSVAPSHDDHVSIAPKTLSGFAYSGHIFWDTEIFILPFLTFTQP
CCCCEEEEEHHHHHHHHHCCCCCCCCEEECCCHHCCEEEECEEEECCCEEEEEHHHHCCH
ALARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESATTGDEVTPRWVPGANGELVRIWCG
HHHHHHHHHHEECCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEC
DIEVHITADVAYAVWHYWQTTDDDEWMHDYGAEIILDTAVFWESRVQWNQQRHSYDILDV
CEEEEEEEHHHEEHHHHHCCCCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEE
IGPDENHDRVDNNAFTNLMVQWHLQSALALWDWFKHVYPEKAAQLVKQLNLTTERLHRWA
CCCCCCCCCCCCCHHHHHEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
EIQERIFVNQDITTGLIEQFEGFFQLEDVNLADYEPRTKSLQGLLGIEATSQKQILKQPD
HHHHHEECCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCC
VLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLGPAIHAVLACDLNNPTEAYTHFLR
HHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHEEEEEEECCCCHHHHHHHHHH
AALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVRMTQFGPMACPNLPSTWKRLKFRL
HHHHCHHHHEEECCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHEEEE
QWRNEWYEFDLQPETAMSVVTQEASVPVFATVQAVMVNG
EECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA