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The map label for this gene is hisS

Identifier: 75908477

GI number: 75908477

Start: 2816637

End: 2818025

Strand: Direct

Name: hisS

Synonym: Ava_2260

Alternate gene names: 75908477

Gene position: 2816637-2818025 (Clockwise)

Preceding gene: 75908476

Following gene: 75908478

Centisome position: 44.25

GC content: 39.52

Gene sequence:

>1389_bases
ATGGCAAAAAACGACAAAATTAACTTCTCAACTCCTAGCGGTTTTCCTGAATTTCTGCCTAGCGAAAAGCGCCTAGAATT
ATATCTACTAGATACCATCCGTAGAGTTTATGAAAGCTATGGATTTACACCTATTGAAACTCCCGCAGTTGAACGTTTAG
AAGTACTACAAGCTAAAGGTAATCAAGGGGATAATATTATTTATGGTATCGACCCAATTCTGCCACCAAACCGCCAAGCA
GAAAAAGATAAATCTGGGGAAACAGGCTCAGAAGCCAGAGCCTTAAAATTTGACCAAACTGTGCCTTTAGCAGCTTATAT
TGCCCGTCACTTAAATGAATTAACTTTTCCCTTTGCCCGTTATCAAATGGACGTGGTTTTTCGCGGAGAAAGAGCCAAGG
ATGGACGATTTCGTCAATTTCGCCAATGTGATATCGATGTAGTTGGACGAGAAAAACTCAGCTTGCTCTATGATGCTCAA
ATGCCGGCAATTATCACCGAAATATTTGAAGCAGTTCATATTGGTGATTTTTTAATTCGCATCAATAACCGAAAAGTTTT
AACAGGATTTTTCCAATCTTTAGATATATCAGAAACGCAAATTAAATCTTGTATTAGCATTATCGATAATCTCGAAAAAA
TCGGTGAAGCTAAAGTTAAATTAGAGTTAGAGAAAGAAGGTATTAATCCAGAGCAAACACAAAAAATCATAGACTTTATC
AAAATCGATGGTAGCGTTGATGATGTATTAGATAAACTCAAACATCTCTCACAAACCCTGCCAGAATCAGAGCAATTTAA
TCTAGGTGTCAGCGAATTAGAAACAGTAATTACAGGCGTGCGTAACTTGGGAGTTCCTGACAAACGCTTTTGTATTGATT
TAGCGATCGCTCGTGGGCTAAATTACTATACAGGCACAGTTTACGAAACAACGCTGATAGGACACGAAGCTTTAGGGAGC
ATTTGTTCGGGTGGCAGATATGAAGAGTTAGTCGGCACATTTATCGGTGAGAAAATGCCAGGTGTGGGTATTTCCATCGG
CTTAACTCGCTTAATTAGCCGCTTACTGAAAGCAGGTATTCTTAACACCTTACCGCCTACTCCAGCACAGGTAGTGGTAG
TAAATATGCAGGATAACCTCATGCCAACTTATTTAAAAGTTTCGCAACAGTTGCGTCAAGCAGGACTGAATGTAATTACA
AACTTTGAGAAACGCCAGTTAGGTAAGCAATTTCAAGCAGCAGATAAACAAGGCATTCAATTTTGTGTCATCATCGGTGC
TGATGAAGCCGCAGCACAAAAGTCATCCCTCAAAGACTTAAAGTCTGGTGAGCAAGTAGAAGTAGCACTAGCAGATTTAC
CGGAAGAAGTTAAACGCAGACTAACGTAA

Upstream 100 bases:

>100_bases
CCCAGGTTGTACAGCCAAAACATTCCCCGACTACTTTACTCGATTCTTTAAAATGATTGGGCAATAGTCAGTTGTCAATT
GATTTAACAAAAATAGACGC

Downstream 100 bases:

>100_bases
ATTTTAAATTGCACAGTAGCCTACACTCTACCATAGGCGATCGCATCCCTTAAATTTAGCTGTTGACTGTCTTCAAGATA
GATTGTGCTACTTAGAAACA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MAKNDKINFSTPSGFPEFLPSEKRLELYLLDTIRRVYESYGFTPIETPAVERLEVLQAKGNQGDNIIYGIDPILPPNRQA
EKDKSGETGSEARALKFDQTVPLAAYIARHLNELTFPFARYQMDVVFRGERAKDGRFRQFRQCDIDVVGREKLSLLYDAQ
MPAIITEIFEAVHIGDFLIRINNRKVLTGFFQSLDISETQIKSCISIIDNLEKIGEAKVKLELEKEGINPEQTQKIIDFI
KIDGSVDDVLDKLKHLSQTLPESEQFNLGVSELETVITGVRNLGVPDKRFCIDLAIARGLNYYTGTVYETTLIGHEALGS
ICSGGRYEELVGTFIGEKMPGVGISIGLTRLISRLLKAGILNTLPPTPAQVVVVNMQDNLMPTYLKVSQQLRQAGLNVIT
NFEKRQLGKQFQAADKQGIQFCVIIGADEAAAQKSSLKDLKSGEQVEVALADLPEEVKRRLT

Sequences:

>Translated_462_residues
MAKNDKINFSTPSGFPEFLPSEKRLELYLLDTIRRVYESYGFTPIETPAVERLEVLQAKGNQGDNIIYGIDPILPPNRQA
EKDKSGETGSEARALKFDQTVPLAAYIARHLNELTFPFARYQMDVVFRGERAKDGRFRQFRQCDIDVVGREKLSLLYDAQ
MPAIITEIFEAVHIGDFLIRINNRKVLTGFFQSLDISETQIKSCISIIDNLEKIGEAKVKLELEKEGINPEQTQKIIDFI
KIDGSVDDVLDKLKHLSQTLPESEQFNLGVSELETVITGVRNLGVPDKRFCIDLAIARGLNYYTGTVYETTLIGHEALGS
ICSGGRYEELVGTFIGEKMPGVGISIGLTRLISRLLKAGILNTLPPTPAQVVVVNMQDNLMPTYLKVSQQLRQAGLNVIT
NFEKRQLGKQFQAADKQGIQFCVIIGADEAAAQKSSLKDLKSGEQVEVALADLPEEVKRRLT
>Mature_461_residues
AKNDKINFSTPSGFPEFLPSEKRLELYLLDTIRRVYESYGFTPIETPAVERLEVLQAKGNQGDNIIYGIDPILPPNRQAE
KDKSGETGSEARALKFDQTVPLAAYIARHLNELTFPFARYQMDVVFRGERAKDGRFRQFRQCDIDVVGREKLSLLYDAQM
PAIITEIFEAVHIGDFLIRINNRKVLTGFFQSLDISETQIKSCISIIDNLEKIGEAKVKLELEKEGINPEQTQKIIDFIK
IDGSVDDVLDKLKHLSQTLPESEQFNLGVSELETVITGVRNLGVPDKRFCIDLAIARGLNYYTGTVYETTLIGHEALGSI
CSGGRYEELVGTFIGEKMPGVGISIGLTRLISRLLKAGILNTLPPTPAQVVVVNMQDNLMPTYLKVSQQLRQAGLNVITN
FEKRQLGKQFQAADKQGIQFCVIIGADEAAAQKSSLKDLKSGEQVEVALADLPEEVKRRLT

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=473, Percent_Identity=33.4038054968288, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI15029520, Length=481, Percent_Identity=30.5613305613306, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1788861, Length=464, Percent_Identity=24.5689655172414, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71993693, Length=485, Percent_Identity=28.8659793814433, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71993686, Length=453, Percent_Identity=29.8013245033113, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=29.1139240506329, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24643061, Length=489, Percent_Identity=29.8568507157464, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24643059, Length=508, Percent_Identity=28.9370078740157, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ANAVT (Q3MAV8)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322773.1
- ProteinModelPortal:   Q3MAV8
- SMR:   Q3MAV8
- STRING:   Q3MAV8
- GeneID:   3679005
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2260
- NMPDR:   fig|240292.3.peg.2527
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   YVAEHEH
- PhylomeDB:   Q3MAV8
- ProtClustDB:   PRK00037
- BioCyc:   AVAR240292:AVA_2260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 51556; Mature: 51425

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNDKINFSTPSGFPEFLPSEKRLELYLLDTIRRVYESYGFTPIETPAVERLEVLQAKG
CCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
NQGDNIIYGIDPILPPNRQAEKDKSGETGSEARALKFDQTVPLAAYIARHLNELTFPFAR
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHH
YQMDVVFRGERAKDGRFRQFRQCDIDVVGREKLSLLYDAQMPAIITEIFEAVHIGDFLIR
EEEEEEEECCCCCCCHHHHHHHCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
INNRKVLTGFFQSLDISETQIKSCISIIDNLEKIGEAKVKLELEKEGINPEQTQKIIDFI
ECCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
KIDGSVDDVLDKLKHLSQTLPESEQFNLGVSELETVITGVRNLGVPDKRFCIDLAIARGL
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NYYTGTVYETTLIGHEALGSICSGGRYEELVGTFIGEKMPGVGISIGLTRLISRLLKAGI
CCCCCCEEEEHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LNTLPPTPAQVVVVNMQDNLMPTYLKVSQQLRQAGLNVITNFEKRQLGKQFQAADKQGIQ
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCE
FCVIIGADEAAAQKSSLKDLKSGEQVEVALADLPEEVKRRLT
EEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHCC
>Mature Secondary Structure 
AKNDKINFSTPSGFPEFLPSEKRLELYLLDTIRRVYESYGFTPIETPAVERLEVLQAKG
CCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
NQGDNIIYGIDPILPPNRQAEKDKSGETGSEARALKFDQTVPLAAYIARHLNELTFPFAR
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHH
YQMDVVFRGERAKDGRFRQFRQCDIDVVGREKLSLLYDAQMPAIITEIFEAVHIGDFLIR
EEEEEEEECCCCCCCHHHHHHHCCCEEECHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEE
INNRKVLTGFFQSLDISETQIKSCISIIDNLEKIGEAKVKLELEKEGINPEQTQKIIDFI
ECCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
KIDGSVDDVLDKLKHLSQTLPESEQFNLGVSELETVITGVRNLGVPDKRFCIDLAIARGL
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NYYTGTVYETTLIGHEALGSICSGGRYEELVGTFIGEKMPGVGISIGLTRLISRLLKAGI
CCCCCCEEEEHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LNTLPPTPAQVVVVNMQDNLMPTYLKVSQQLRQAGLNVITNFEKRQLGKQFQAADKQGIQ
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCE
FCVIIGADEAAAQKSSLKDLKSGEQVEVALADLPEEVKRRLT
EEEEECCCHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA