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The map label for this gene is algI [H]

Identifier: 75908356

GI number: 75908356

Start: 2642756

End: 2644255

Strand: Direct

Name: algI [H]

Synonym: Ava_2137

Alternate gene names: 75908356

Gene position: 2642756-2644255 (Clockwise)

Preceding gene: 75908355

Following gene: 75908357

Centisome position: 41.52

GC content: 40.73

Gene sequence:

>1500_bases
ATGAACTTTATATCTATTTTGTATGGGCTTTTCTTGTTAAGTGCGCTGGGGATTTATTGGTCTGTAGGTCGTAAGCAACT
GCGATTGTGGACTTTATTGATTGCTAGCCTGGTGTTTTATGCTTCTTTGAGTATCCAATACATCCCACTGTTATTAACAC
TCACATTTATTAACTTCCGCTTAGGGCGAGAAATTGGTAAAAACACATCACCAGGACAACATAACCTCAACTGGCAAATT
TCTAATGAAGAGTGGCAATTTGCTCAAGTAGATTGGAACCGCAAACGGCTAAATTTATTGTGGCTAGGTGTGGTTTTAAA
TGTTGTATTATTACTCGGATTTAAGTATATTAACAGTTTCTTGCAGTTAATTTTTGATTTTCCCGTTAACCCATCAGAGT
CATCTTGGAAAATTATTGCACCTTTAGGAATTTCATTTTTTACATTTGAATGTATTGCTTATTTAATAGATGTTTATCGT
GGTGCGCCAGCCACTAATAATTTTCTCCAATTCGCTACCTATAAATTATTTTTTGCTAAATTAATTTCTGGCCCCATTAC
CCGTTATCACAACCTAGCAAATCAATTTAATACCTTAGAGTTACCCACTGCTGATAAGGTGTCAGAGGCATTATGGTTAA
TTGCTAGGGGTGCTGTCAAAAAAGGGATTTTGGCAGACAGGCTAGGGATTTTTGTAGATTTGTGTTTCGGTAATTTGCCA
CGGGCTGGTAGTACTGATCTTTGGCTAGCTACCTTCGCTTATGGTTTGCAGTTGTATTTGGATTTTAATGGTTATGTAGA
CATCGCCCGTGGTACTGCTTTGCTATTTGGTTTAGTGCTACCTGAAAACTTTGATTTTCCCTATTTCACCACCAGCATCG
CCGAATTTTGGCGGCGTTGGCACATGACTCTAGGAGATTGGTTGCGGAATTATGTCTACTTTCCTTTGGGGGGTTCCCGT
CAAGGTTTAACTCGCACCAGCGGGAATTTGTTCTTGATTATGCTAATCGCTGGGATTTGGCACGGTTCCGCCTGGGGCTT
TGTGGTTTGGGGGATGTATCATGGTTTAGCTTTAGTGGTTCATCGACTGACGGATGTAATGAGCGATCGCTACGAAAAGT
TAGAGCTATTCTGGCAAAATCCTTTAGGGGTAGTTGTGGCTTGGCTATTCACCCAATGGATGGTTTTCACTTCTTGGATT
TGGTTCCGTCTGCCTAATCTGCCAGAATCTTCTCTCGTCATTCAGCGTCTCTGGGGTCACTCTGCGGATGCCCAATTTGC
ACAAAAAGTCTACGTGGAAGCCTTAAATACTAGCCAATCTCAAGTGTCTGCCATTCTTATAGCTTTATTTACTCTCATGG
GTATAACCTATGGCTTCAAAGGCAAGCTGAAACTAGAGTTAAACTGGCCTCTAAAACTCGTCTTTGTACCTCTATGCCTC
TACGCTGTTTGGTTATTCGCCCCTGAAGGTAGCCTTCCTTATATTTACTTTGATTTTTAA

Upstream 100 bases:

>100_bases
GGAAGCAGGGGAAGCAGGGGAAGCAGGGGAAGTAAAGAAGAATACTATGGACTATTGACCAATGACAACTGACAACTGAC
AACTGACAACTGACTAAACC

Downstream 100 bases:

>100_bases
TAGTTTTCTCCCTAAAACGACGTAAATCAATAAATGTTTGCCGTCAGGAGTTTAGTCTCAAAAAAGGACTTTAGCTCTTA
TACCAATTCTCTATAAAGTT

Product: membrane bound O-acyl transferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNFISILYGLFLLSALGIYWSVGRKQLRLWTLLIASLVFYASLSIQYIPLLLTLTFINFRLGREIGKNTSPGQHNLNWQI
SNEEWQFAQVDWNRKRLNLLWLGVVLNVVLLLGFKYINSFLQLIFDFPVNPSESSWKIIAPLGISFFTFECIAYLIDVYR
GAPATNNFLQFATYKLFFAKLISGPITRYHNLANQFNTLELPTADKVSEALWLIARGAVKKGILADRLGIFVDLCFGNLP
RAGSTDLWLATFAYGLQLYLDFNGYVDIARGTALLFGLVLPENFDFPYFTTSIAEFWRRWHMTLGDWLRNYVYFPLGGSR
QGLTRTSGNLFLIMLIAGIWHGSAWGFVVWGMYHGLALVVHRLTDVMSDRYEKLELFWQNPLGVVVAWLFTQWMVFTSWI
WFRLPNLPESSLVIQRLWGHSADAQFAQKVYVEALNTSQSQVSAILIALFTLMGITYGFKGKLKLELNWPLKLVFVPLCL
YAVWLFAPEGSLPYIYFDF

Sequences:

>Translated_499_residues
MNFISILYGLFLLSALGIYWSVGRKQLRLWTLLIASLVFYASLSIQYIPLLLTLTFINFRLGREIGKNTSPGQHNLNWQI
SNEEWQFAQVDWNRKRLNLLWLGVVLNVVLLLGFKYINSFLQLIFDFPVNPSESSWKIIAPLGISFFTFECIAYLIDVYR
GAPATNNFLQFATYKLFFAKLISGPITRYHNLANQFNTLELPTADKVSEALWLIARGAVKKGILADRLGIFVDLCFGNLP
RAGSTDLWLATFAYGLQLYLDFNGYVDIARGTALLFGLVLPENFDFPYFTTSIAEFWRRWHMTLGDWLRNYVYFPLGGSR
QGLTRTSGNLFLIMLIAGIWHGSAWGFVVWGMYHGLALVVHRLTDVMSDRYEKLELFWQNPLGVVVAWLFTQWMVFTSWI
WFRLPNLPESSLVIQRLWGHSADAQFAQKVYVEALNTSQSQVSAILIALFTLMGITYGFKGKLKLELNWPLKLVFVPLCL
YAVWLFAPEGSLPYIYFDF
>Mature_499_residues
MNFISILYGLFLLSALGIYWSVGRKQLRLWTLLIASLVFYASLSIQYIPLLLTLTFINFRLGREIGKNTSPGQHNLNWQI
SNEEWQFAQVDWNRKRLNLLWLGVVLNVVLLLGFKYINSFLQLIFDFPVNPSESSWKIIAPLGISFFTFECIAYLIDVYR
GAPATNNFLQFATYKLFFAKLISGPITRYHNLANQFNTLELPTADKVSEALWLIARGAVKKGILADRLGIFVDLCFGNLP
RAGSTDLWLATFAYGLQLYLDFNGYVDIARGTALLFGLVLPENFDFPYFTTSIAEFWRRWHMTLGDWLRNYVYFPLGGSR
QGLTRTSGNLFLIMLIAGIWHGSAWGFVVWGMYHGLALVVHRLTDVMSDRYEKLELFWQNPLGVVVAWLFTQWMVFTSWI
WFRLPNLPESSLVIQRLWGHSADAQFAQKVYVEALNTSQSQVSAILIALFTLMGITYGFKGKLKLELNWPLKLVFVPLCL
YAVWLFAPEGSLPYIYFDF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 57394; Mature: 57394

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFISILYGLFLLSALGIYWSVGRKQLRLWTLLIASLVFYASLSIQYIPLLLTLTFINFR
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGREIGKNTSPGQHNLNWQISNEEWQFAQVDWNRKRLNLLWLGVVLNVVLLLGFKYINSF
HHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLIFDFPVNPSESSWKIIAPLGISFFTFECIAYLIDVYRGAPATNNFLQFATYKLFFAK
HHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LISGPITRYHNLANQFNTLELPTADKVSEALWLIARGAVKKGILADRLGIFVDLCFGNLP
HHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGSTDLWLATFAYGLQLYLDFNGYVDIARGTALLFGLVLPENFDFPYFTTSIAEFWRRW
CCCCCHHHHHHHHHCEEEEEEECCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HMTLGDWLRNYVYFPLGGSRQGLTRTSGNLFLIMLIAGIWHGSAWGFVVWGMYHGLALVV
HHHHHHHHHHCEEECCCCCCCCCEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HRLTDVMSDRYEKLELFWQNPLGVVVAWLFTQWMVFTSWIWFRLPNLPESSLVIQRLWGH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SADAQFAQKVYVEALNTSQSQVSAILIALFTLMGITYGFKGKLKLELNWPLKLVFVPLCL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
YAVWLFAPEGSLPYIYFDF
HHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MNFISILYGLFLLSALGIYWSVGRKQLRLWTLLIASLVFYASLSIQYIPLLLTLTFINFR
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGREIGKNTSPGQHNLNWQISNEEWQFAQVDWNRKRLNLLWLGVVLNVVLLLGFKYINSF
HHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LQLIFDFPVNPSESSWKIIAPLGISFFTFECIAYLIDVYRGAPATNNFLQFATYKLFFAK
HHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LISGPITRYHNLANQFNTLELPTADKVSEALWLIARGAVKKGILADRLGIFVDLCFGNLP
HHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGSTDLWLATFAYGLQLYLDFNGYVDIARGTALLFGLVLPENFDFPYFTTSIAEFWRRW
CCCCCHHHHHHHHHCEEEEEEECCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HMTLGDWLRNYVYFPLGGSRQGLTRTSGNLFLIMLIAGIWHGSAWGFVVWGMYHGLALVV
HHHHHHHHHHCEEECCCCCCCCCEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HRLTDVMSDRYEKLELFWQNPLGVVVAWLFTQWMVFTSWIWFRLPNLPESSLVIQRLWGH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
SADAQFAQKVYVEALNTSQSQVSAILIALFTLMGITYGFKGKLKLELNWPLKLVFVPLCL
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
YAVWLFAPEGSLPYIYFDF
HHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]