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The map label for this gene is cbiF [H]

Identifier: 75908189

GI number: 75908189

Start: 2444302

End: 2445087

Strand: Direct

Name: cbiF [H]

Synonym: Ava_1968

Alternate gene names: 75908189

Gene position: 2444302-2445087 (Clockwise)

Preceding gene: 75908188

Following gene: 75908190

Centisome position: 38.4

GC content: 45.17

Gene sequence:

>786_bases
ATGCAAAATCATGTAAATCCCCTAGATTCCGCCGTTTATATTGTAGGAGCGGGACCTGGAGACCCAGATTTACTGACAGT
TAAAGCCCAAAAACTACTGTCAGTAGCAGATGTGATTTTATTCGCAGATTCGTTAATACCAGAGGAGCTTTTACAGTTTT
GCCGAAAAGATGCAGAAATTATTCAGACTGCAAACAAAACTCTGGAGGAGATTATACCTTTGATGATCGCCAGGGTGCGA
TCGCACAAATCTGTAGTACGTCTCCATTCCGGTGATCCCAGTCTCTACAGCGCCATTAATGAGCAAATGCAGCTTCTGGC
AGCAGCTAATATTCCTTTTGAAGTCATTCCCGGTATTAGTGCTTTCCAAGCCGCCGCAGCTAAACTGAAGGTAGAGTTGA
CTGTTCCTGGTTTAGTCCAGACTATTATTCTGACGCGCATTAGTGGACGAACACAAGTCCCACAGACAGAAGAACTAGCT
ACCCTCGCAGCACATCAAGCTAGTTTATGCTTATATCTGAGTGCGCGTCATGTGGAAAATGCCCAAGCTAAACTACTAGA
ACACTATCCCCCAGATACACCTGTAGCTATTTGCTTTCGCATAGGATGGCCTGATGAAAGAATTCGTGTGGTTCCTCTCC
GGGAGATGGCAGAGTGTACTCAGCAAGAAAATCTGATTCGCACTACGTTATATGTGATTAGCCCTGCATTATCACATACC
ACTGGGCGATCGCGCTTATATCATCCTGAGCATAGTCATCTGTTCCGCACTGCTGGAGATAGCTAA

Upstream 100 bases:

>100_bases
CTAGAGGGATAATCCGGAAAGATACCGAGCAAATTCTCAAAAAATTTTTTGATTTTTTTCTGTCTACCGATAATTACTTA
GATTTTATATAAAATTTTGT

Downstream 100 bases:

>100_bases
GGTAAACTCATAATCTGACACGATATAAGTTCTCAAAAGGAAATGTTCAAGCCTTGACTAACCGACTTTTACTGAGGGAA
AAACTACAAGCAAAACTGGC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQNHVNPLDSAVYIVGAGPGDPDLLTVKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SHKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISAFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPQTEELA
TLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDERIRVVPLREMAECTQQENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS

Sequences:

>Translated_261_residues
MQNHVNPLDSAVYIVGAGPGDPDLLTVKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SHKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISAFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPQTEELA
TLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDERIRVVPLREMAECTQQENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS
>Mature_261_residues
MQNHVNPLDSAVYIVGAGPGDPDLLTVKAQKLLSVADVILFADSLIPEELLQFCRKDAEIIQTANKTLEEIIPLMIARVR
SHKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGISAFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPQTEELA
TLAAHQASLCLYLSARHVENAQAKLLEHYPPDTPVAICFRIGWPDERIRVVPLREMAECTQQENLIRTTLYVISPALSHT
TGRSRLYHPEHSHLFRTAGDS

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=227, Percent_Identity=32.15859030837, Blast_Score=113, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322922, Length=222, Percent_Identity=25.2252252252252, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28760; Mature: 28760

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNHVNPLDSAVYIVGAGPGDPDLLTVKAQKLLSVADVILFADSLIPEELLQFCRKDAEI
CCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IQTANKTLEEIIPLMIARVRSHKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGIS
HHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCCH
AFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPQTEELATLAAHQASLCLYLSARHVEN
HHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHH
AQAKLLEHYPPDTPVAICFRIGWPDERIRVVPLREMAECTQQENLIRTTLYVISPALSHT
HHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGRSRLYHPEHSHLFRTAGDS
CCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MQNHVNPLDSAVYIVGAGPGDPDLLTVKAQKLLSVADVILFADSLIPEELLQFCRKDAEI
CCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IQTANKTLEEIIPLMIARVRSHKSVVRLHSGDPSLYSAINEQMQLLAAANIPFEVIPGIS
HHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCCH
AFQAAAAKLKVELTVPGLVQTIILTRISGRTQVPQTEELATLAAHQASLCLYLSARHVEN
HHHHHHHHEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHH
AQAKLLEHYPPDTPVAICFRIGWPDERIRVVPLREMAECTQQENLIRTTLYVISPALSHT
HHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGRSRLYHPEHSHLFRTAGDS
CCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]