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The map label for this gene is sigF [H]

Identifier: 75908065

GI number: 75908065

Start: 2297176

End: 2297952

Strand: Direct

Name: sigF [H]

Synonym: Ava_1844

Alternate gene names: 75908065

Gene position: 2297176-2297952 (Clockwise)

Preceding gene: 75908064

Following gene: 75908066

Centisome position: 36.09

GC content: 42.34

Gene sequence:

>777_bases
ATGCCTACAACAGCTACCAACGAATTGAAATACGAAATTTGGCAGTTGTTACGAGAATATCAGCAATCTCGGTCAGAAAC
TGTTCGTAATCAACTAGTAAAACTGAATCTTGGATTAGTGAGGAAAGAAGCTCACTACTGGATGAATCAATGCCGTGAAA
ACTATGATGATTTAGTTCAGGTTGGTTGTTTGGGTTTAATCAGAGCGATTGAAAGATTTGAAATTGCCAAAGGCCACGCT
TTTAGCTCCTTTGCTATTCCTTATATTCGTGGCGAAATTCAGCACTACCTCCGTGATAAAGGTGTAACAGTAAGAATTCC
TCGTCGCTACTTGGCTATACAACAGCGAGCGATTGGGGTTTCGCGTTCTTTGCGGGAACAATACAACCGCCAACCAACTG
ACTCAGAATTAGCAACAGCACTGGATATTTCTCCAGATGAGTGGCAGGAAATCAAATTAGCATGGGTAAATCGCGCTCCT
TTGAGTTTAGATGTACCAGTTCAAGATGGTGAAGAAGGAGCAACTAGCCTGGGAGAATTGGTTCCTGATCATCATTACCG
CAGTTTTCAGTTGGCGCAAGAAGACCAAATTCGCTTACAACAAGCATTGTTCCAGTTAGAGCAACGCACTCGTGAAGTTT
TAGAGTGTGTGTTTTTACAAGACTTGACGCAGAAACAGGTGGCAGAACACTTAGGAATTAGTGTCGTCACAGTTTCCAGG
AGAGTCAAAAAAGGGTTGGACTTGTTAAAGCATCTTATGTGTGTGGCAGACGATTAA

Upstream 100 bases:

>100_bases
CACAGAGCAAATAATTTAATATTTGCGTTGATAGCGCTTAATCGCAGCCTTATTTAAAAGCGATTACTCAAATAATTATT
TACTTAATAAATGCGGTGCT

Downstream 100 bases:

>100_bases
AGTTCCATTAAGTCATCAGCATTTATTCGGAATTATTAGTGTCAAAAACAACTCTTTAAATCCCAAAAAATTAGGTTATA
AAGGAAACGACAACAATGCC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHA
FSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALDISPDEWQEIKLAWVNRAP
LSLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
RVKKGLDLLKHLMCVADD

Sequences:

>Translated_258_residues
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHA
FSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALDISPDEWQEIKLAWVNRAP
LSLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
RVKKGLDLLKHLMCVADD
>Mature_257_residues
PTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHAF
SSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALDISPDEWQEIKLAWVNRAPL
SLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSRR
VKKGLDLLKHLMCVADD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=26.1603375527426, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789098, Length=232, Percent_Identity=26.2931034482759, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788231, Length=188, Percent_Identity=30.8510638297872, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29926; Mature: 29794

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCLGLIRAIERFEIAKGHAFSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGV
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
SRSLREQYNRQPTDSELATALDISPDEWQEIKLAWVNRAPLSLDVPVQDGEEGATSLGEL
HHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCCCCCHHHHHHHHH
VPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVKKGLDLLKHLMCVADD
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCLGLIRAIERFEIAKGHAFSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGV
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
SRSLREQYNRQPTDSELATALDISPDEWQEIKLAWVNRAPLSLDVPVQDGEEGATSLGEL
HHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCCCCCHHHHHHHHH
VPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVKKGLDLLKHLMCVADD
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]