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The map label for this gene is cphA

Identifier: 75908035

GI number: 75908035

Start: 2258789

End: 2261494

Strand: Direct

Name: cphA

Synonym: Ava_1814

Alternate gene names: 75908035

Gene position: 2258789-2261494 (Clockwise)

Preceding gene: 75908034

Following gene: 75908038

Centisome position: 35.48

GC content: 49.04

Gene sequence:

>2706_bases
ATGAGAATCCTCAAGATCCAGACCTTACGCGGCCCAAACTACTGGAGCATTCGACGCCACAAACTGATCGTCATGCGCCT
CGATTTGGAAACCCTTGCCGAGACGCCATCAAATGAAATCCCCGGATTTTATGAAGGATTAGTAGAGGCGCTGCCAAGTC
TGGAAGGTCATTATTGCTCGCCCGGCTGTCATGGTGGTTTTTTGATGAGAGTGCGAGAAGGCACCATGATGGGTCATATC
GTGGAACACGTAGCCCTAGAACTCCAAGAATTAGCTGGAATGCACGTGGGCTTTGGTCGTACCCGTGAAACTGCCACACC
TGGAATTTATCAGGTAGTAATCGAGTATTTAAACGAGGAAGCGGGACGCTATGCTGGACGAGCAGCTGTCAGGTTGTGCC
AAAGCATTGTAGATCGCGGTCGATACCCCAAGGCAGAACTAGAGCAAGATATACAAGACCTGAAAGATTTATGGCGTGAT
GCTTCCCTGGGTCCCTCCACCGAAGCAATTGTCAAAGAAGCCGAGAAACGTGGTATCCCTTGGATGCAGCTGAGCGCACG
CTTTTTGATTCAACTAGGCTATGGCGTGAATCATAAGCGGATGCAGGCCACCATGACCGATAAAACGGGTATTTTAGGCG
TAGAACTAGCTTGCGATAAAGAAGCAACCAAACGCATCTTAGCCGCATCTGGTGTGCCGGTACCCAGGGGTACAGTGATT
AATTTCTTGGATGATTTGGAAGAAGCCATCGAATATGTTGGCGGTTATCCGATTGTCATCAAGCCCCTAGATGGCAATCA
TGGACGCGGTATTACAATCGATATTAGAAGTTGGGAAGAAGCTGAAGCCGCTTACGAAGCAGCGAGACAAGTTTCCCGGT
CAATCATTGTTGAGCGATATTACGTTGGGCGCGACCACAGAGTACTAGTGGTAGATGGTAAAGTCGTGGCCGTTGCCGAA
CGTGTACCAGCTCATGTGATTGGTAACGGTCGCTCCACCATCGCCGAACTAATAGAGGAAATCAACCAAGACCCCAACCG
TGGCGACGGACACGATAAAGTCCTCACTAAAATAGAACTAGACCGTACCAGTTACCAACTACTCGAAAGGGCAGGTTACA
CACTCAACAGCGTGCCACCAAAAGGCACGATTTGTTATTTAAGGGCAACCGCCAACCTGAGTACAGGTGGTACTGCTGTA
GACAGGACAGACGAAATTCACCCAGAAAACATCTGGTTAGCCCAAAGAGTAGTCAAGATTATCGGTTTGGACATCGCCGG
ACTCGATATCGTCACTACCGACATTAGCCGCCCCTTGAGGGAATTAGACGGTGTAATTGTCGAAGTCAACGCTGCGCCGG
GCTTTAGAATGCACGTAGCTCCTAGCCAAGGCATTCCCCGCAACGTTGCCGGCGCAGTGATGGATATGCTCTTCCCCAAC
GAACAGTCCGGCCGCATTCCCATCCTCAGTGTTACCGGCACTAACGGTAAAACCACCACTACCCGCCTGCTGGCACACAT
TTATAAGCAAACCGGAAAAGTAGTAGGCTATACAACTACTGATGGAACATATATCGGTGATTACTTAGTTGAATCTGGTG
ATAACACTGGGCCTCAAAGCGCTCACGTCATCTTACAAGACCCAACCGTCGAGGTTGCAGTTTTGGAAACAGCCCGTGGC
GGTATTTTGCGCTCTGGCTTAGGCTTTGAGTCTGCTAATGTGGGTGTGGTGTTAAATGTGGCGGCAGATCACTTAGGAAT
TGGTGACATTGACACTATTGATCAGTTGGCTAACCTGAAAAGCGTGGTGGCGGAATCTGTGTATCCTGATGGCTATGCAG
TACTCAACGCCGACGATCGCCGCGTTGCTGCAATGGCAGAAAAAACCAAAGCCAATATCGCTTACTTCACCATGAATCCC
GACTCGGAACTGGTGCGGAAACATATCCAGAAGGGCGGAGTTGCAGCAGTCTATGAAAATGGCTATCTGTCAATCGTCAA
AGGCGATTGGACACACCGCATCGAAAGGGCAGAACAAATCCCCTTGACAATGGGTGGACGCGCACCATTTATGATCGCTA
ACGCCTTAGCCGCGAGTTTGGCAGCTTTTGTACAGAACGTCTCCATTGAGCAAATTCGTGCTGGCTTAAGAACCTTCCGG
GCTTCCGTGAGTCAAACCCCAGGACGGATGAACTTATTCAATTTGGGCAACTACCACGCCTTAGTTGACTATGCTCACAA
CCCAGCTAGTTACGAAGCTGTAGGCGCATTTGTACGTAACTGGACAAGTGGACAACGTATTGGTGTAGTCGGTGGGCCTG
GCGATCGCCGGGACGAAGATTTTGTCACCTTAGGCAAACTAGCCGCCGAGATTTTTGATTACATCATTGTCAAAGAAGAC
GACGATACTAGAGGTAGACCAAGGGGTTCAGCCTCTGCATTGATAACCAAAGGCATCACCCAAGTTAAGCCAGATGCTCG
TTATGAGTCCATTCTGGATGAAACACAAGCAATTAACAAGGGCTTGGATATGGCTCCCGCGAACGGTTTGGTGGTCATCC
TACCGGAAAGCGTTAGCCGTGCTATTAAGTTAATTAAACTGCGTGGATTAGTCAAAGAGGAGATACAGCAACAAAATCCC
TCGACAACCGTTATAGATAACCAAAATGGCGTAGCATCTTCTTCTGTAATTAATACTTTGCTGTAG

Upstream 100 bases:

>100_bases
ACCATGTAAAAAGATAAAAACTGTTTTTCATGAACAGTTTAGCGATCAATTCCAGTAAGAAACTAGAATTTTGGTTCCGA
ATCTCCATCTACCTATTCCC

Downstream 100 bases:

>100_bases
TCGATAGTAGAGATGTGGTCATTCCCATCTCCACTATTGACCGTTGCTGAATCTTCTTAATTGGAATTTCTTATTTTTCT
TCTTCCGGGTTGGTATCCTC

Product: cyanophycin synthetase

Products: NA

Alternate protein names: Cyanophycin synthase

Number of amino acids: Translated: 901; Mature: 901

Protein sequence:

>901_residues
MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCSPGCHGGFLMRVREGTMMGHI
VEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEEAGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRD
ASLGPSTEAIVKEAEKRGIPWMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI
NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERYYVGRDHRVLVVDGKVVAVAE
RVPAHVIGNGRSTIAELIEEINQDPNRGDGHDKVLTKIELDRTSYQLLERAGYTLNSVPPKGTICYLRATANLSTGGTAV
DRTDEIHPENIWLAQRVVKIIGLDIAGLDIVTTDISRPLRELDGVIVEVNAAPGFRMHVAPSQGIPRNVAGAVMDMLFPN
EQSGRIPILSVTGTNGKTTTTRLLAHIYKQTGKVVGYTTTDGTYIGDYLVESGDNTGPQSAHVILQDPTVEVAVLETARG
GILRSGLGFESANVGVVLNVAADHLGIGDIDTIDQLANLKSVVAESVYPDGYAVLNADDRRVAAMAEKTKANIAYFTMNP
DSELVRKHIQKGGVAAVYENGYLSIVKGDWTHRIERAEQIPLTMGGRAPFMIANALAASLAAFVQNVSIEQIRAGLRTFR
ASVSQTPGRMNLFNLGNYHALVDYAHNPASYEAVGAFVRNWTSGQRIGVVGGPGDRRDEDFVTLGKLAAEIFDYIIVKED
DDTRGRPRGSASALITKGITQVKPDARYESILDETQAINKGLDMAPANGLVVILPESVSRAIKLIKLRGLVKEEIQQQNP
STTVIDNQNGVASSSVINTLL

Sequences:

>Translated_901_residues
MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCSPGCHGGFLMRVREGTMMGHI
VEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEEAGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRD
ASLGPSTEAIVKEAEKRGIPWMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI
NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERYYVGRDHRVLVVDGKVVAVAE
RVPAHVIGNGRSTIAELIEEINQDPNRGDGHDKVLTKIELDRTSYQLLERAGYTLNSVPPKGTICYLRATANLSTGGTAV
DRTDEIHPENIWLAQRVVKIIGLDIAGLDIVTTDISRPLRELDGVIVEVNAAPGFRMHVAPSQGIPRNVAGAVMDMLFPN
EQSGRIPILSVTGTNGKTTTTRLLAHIYKQTGKVVGYTTTDGTYIGDYLVESGDNTGPQSAHVILQDPTVEVAVLETARG
GILRSGLGFESANVGVVLNVAADHLGIGDIDTIDQLANLKSVVAESVYPDGYAVLNADDRRVAAMAEKTKANIAYFTMNP
DSELVRKHIQKGGVAAVYENGYLSIVKGDWTHRIERAEQIPLTMGGRAPFMIANALAASLAAFVQNVSIEQIRAGLRTFR
ASVSQTPGRMNLFNLGNYHALVDYAHNPASYEAVGAFVRNWTSGQRIGVVGGPGDRRDEDFVTLGKLAAEIFDYIIVKED
DDTRGRPRGSASALITKGITQVKPDARYESILDETQAINKGLDMAPANGLVVILPESVSRAIKLIKLRGLVKEEIQQQNP
STTVIDNQNGVASSSVINTLL
>Mature_901_residues
MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCSPGCHGGFLMRVREGTMMGHI
VEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEEAGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRD
ASLGPSTEAIVKEAEKRGIPWMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI
NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERYYVGRDHRVLVVDGKVVAVAE
RVPAHVIGNGRSTIAELIEEINQDPNRGDGHDKVLTKIELDRTSYQLLERAGYTLNSVPPKGTICYLRATANLSTGGTAV
DRTDEIHPENIWLAQRVVKIIGLDIAGLDIVTTDISRPLRELDGVIVEVNAAPGFRMHVAPSQGIPRNVAGAVMDMLFPN
EQSGRIPILSVTGTNGKTTTTRLLAHIYKQTGKVVGYTTTDGTYIGDYLVESGDNTGPQSAHVILQDPTVEVAVLETARG
GILRSGLGFESANVGVVLNVAADHLGIGDIDTIDQLANLKSVVAESVYPDGYAVLNADDRRVAAMAEKTKANIAYFTMNP
DSELVRKHIQKGGVAAVYENGYLSIVKGDWTHRIERAEQIPLTMGGRAPFMIANALAASLAAFVQNVSIEQIRAGLRTFR
ASVSQTPGRMNLFNLGNYHALVDYAHNPASYEAVGAFVRNWTSGQRIGVVGGPGDRRDEDFVTLGKLAAEIFDYIIVKED
DDTRGRPRGSASALITKGITQVKPDARYESILDETQAINKGLDMAPANGLVVILPESVSRAIKLIKLRGLVKEEIQQQNP
STTVIDNQNGVASSSVINTLL

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer)

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CPHA_ANAVT (O86109)

Other databases:

- EMBL:   AJ005201
- EMBL:   CP000117
- RefSeq:   YP_322331.1
- ProteinModelPortal:   O86109
- SMR:   O86109
- STRING:   O86109
- GeneID:   3681922
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_1814
- NMPDR:   fig|240292.3.peg.2894
- eggNOG:   COG1181
- HOGENOM:   HBG288839
- OMA:   GPNLWSR
- ProtClustDB:   PRK14016
- BioCyc:   AVAR240292:AVA_1814-MONOMER
- BRENDA:   6.3.2.29
- BRENDA:   6.3.2.30
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR02068

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.29; =6.3.2.30

Molecular weight: Translated: 98267; Mature: 98267

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCS
CEEEEEEEECCCCEEEEEEEEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC
PGCHGGFLMRVREGTMMGHIVEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEE
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRDASLGPSTEAIVKEAEKRGIP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
WMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI
HHHHHHHHHEEECCCCCHHHEEEEECCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCHHH
NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERY
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHE
YVGRDHRVLVVDGKVVAVAERVPAHVIGNGRSTIAELIEEINQDPNRGDGHDKVLTKIEL
EECCCCEEEEECCCEEEEEHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHEEEEEEE
DRTSYQLLERAGYTLNSVPPKGTICYLRATANLSTGGTAVDRTDEIHPENIWLAQRVVKI
CHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IGLDIAGLDIVTTDISRPLRELDGVIVEVNAAPGFRMHVAPSQGIPRNVAGAVMDMLFPN
HCCCCCCCEEEEHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCC
EQSGRIPILSVTGTNGKTTTTRLLAHIYKQTGKVVGYTTTDGTYIGDYLVESGDNTGPQS
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHCCCCCCCCCC
AHVILQDPTVEVAVLETARGGILRSGLGFESANVGVVLNVAADHLGIGDIDTIDQLANLK
CEEEEECCCEEEEEEECCCCCCEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
SVVAESVYPDGYAVLNADDRRVAAMAEKTKANIAYFTMNPDSELVRKHIQKGGVAAVYEN
HHHHHHCCCCCEEEECCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEEEC
GYLSIVKGDWTHRIERAEQIPLTMGGRAPFMIANALAASLAAFVQNVSIEQIRAGLRTFR
CEEEEEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ASVSQTPGRMNLFNLGNYHALVDYAHNPASYEAVGAFVRNWTSGQRIGVVGGPGDRRDED
HHHHCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCH
FVTLGKLAAEIFDYIIVKEDDDTRGRPRGSASALITKGITQVKPDARYESILDETQAINK
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHC
GLDMAPANGLVVILPESVSRAIKLIKLRGLVKEEIQQQNPSTTVIDNQNGVASSSVINTL
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHC
L
C
>Mature Secondary Structure
MRILKIQTLRGPNYWSIRRHKLIVMRLDLETLAETPSNEIPGFYEGLVEALPSLEGHYCS
CEEEEEEEECCCCEEEEEEEEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCC
PGCHGGFLMRVREGTMMGHIVEHVALELQELAGMHVGFGRTRETATPGIYQVVIEYLNEE
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AGRYAGRAAVRLCQSIVDRGRYPKAELEQDIQDLKDLWRDASLGPSTEAIVKEAEKRGIP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
WMQLSARFLIQLGYGVNHKRMQATMTDKTGILGVELACDKEATKRILAASGVPVPRGTVI
HHHHHHHHHEEECCCCCHHHEEEEECCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCHHH
NFLDDLEEAIEYVGGYPIVIKPLDGNHGRGITIDIRSWEEAEAAYEAARQVSRSIIVERY
HHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHE
YVGRDHRVLVVDGKVVAVAERVPAHVIGNGRSTIAELIEEINQDPNRGDGHDKVLTKIEL
EECCCCEEEEECCCEEEEEHHCCCEEECCCHHHHHHHHHHHCCCCCCCCCCHHEEEEEEE
DRTSYQLLERAGYTLNSVPPKGTICYLRATANLSTGGTAVDRTDEIHPENIWLAQRVVKI
CHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IGLDIAGLDIVTTDISRPLRELDGVIVEVNAAPGFRMHVAPSQGIPRNVAGAVMDMLFPN
HCCCCCCCEEEEHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCC
EQSGRIPILSVTGTNGKTTTTRLLAHIYKQTGKVVGYTTTDGTYIGDYLVESGDNTGPQS
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHCCCCCCCCCC
AHVILQDPTVEVAVLETARGGILRSGLGFESANVGVVLNVAADHLGIGDIDTIDQLANLK
CEEEEECCCEEEEEEECCCCCCEECCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
SVVAESVYPDGYAVLNADDRRVAAMAEKTKANIAYFTMNPDSELVRKHIQKGGVAAVYEN
HHHHHHCCCCCEEEECCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEEEC
GYLSIVKGDWTHRIERAEQIPLTMGGRAPFMIANALAASLAAFVQNVSIEQIRAGLRTFR
CEEEEEECCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ASVSQTPGRMNLFNLGNYHALVDYAHNPASYEAVGAFVRNWTSGQRIGVVGGPGDRRDED
HHHHCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCH
FVTLGKLAAEIFDYIIVKEDDDTRGRPRGSASALITKGITQVKPDARYESILDETQAINK
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHC
GLDMAPANGLVVILPESVSRAIKLIKLRGLVKEEIQQQNPSTTVIDNQNGVASSSVINTL
CCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9652408