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The map label for this gene is nth [H]

Identifier: 75907952

GI number: 75907952

Start: 2166340

End: 2167011

Strand: Direct

Name: nth [H]

Synonym: Ava_1731

Alternate gene names: 75907952

Gene position: 2166340-2167011 (Clockwise)

Preceding gene: 75907951

Following gene: 75907953

Centisome position: 34.03

GC content: 45.39

Gene sequence:

>672_bases
GTGAGTACTACTACCCGTAAATCACCTTCTAAAAAGCAACGGGCGTTAGAAGTTCTCTCTCGCCTGAAGCGTCTTTATCC
CGATGCCACCTGCTCCTTAAATTACACCACAACAGTACAGTTGCTGGTGGCAACTATTCTCTCAGCCCAGTGTACGGATG
AGCGAGTCAATCTAGTTACACCAGCTTTATTTAGCCGCTTTCCTGATGCGCCTAGTCTAGCGAATGCGGATTTAACAGAG
TTAGAAAACTTGGTACGTTCCACAGGGTTTTATCGCAATAAAGCCAAAAATATTCAAGCCGCCTGTCGCATGATTGTTAG
TGAGTATAACTCTGTGGTTCCCAACACAATGGAACAGTTGCTTAAACTGCCTGGTGTGGCGAGAAAAACGGCAAATGTGG
TTTTAGCTCATGCCTATGGTATCAATGCTGGGGTGACAGTAGATACCCATGTGAAGCGCCTCAGCCAACGCTTAGGTTTA
ACTAAATATCCAGACCCCGTTCACATTGAACAGGATTTAATGAAATTACTGCCCCAACCAGATTGGGAAAATTGGTCAAT
TCGGCTAATTTATCACGGTCGCGCTGTCTGTAAAGCCCGTTCTCCTGTATGTGAGGCTTGCGAATTGGCGGATTTATGTC
CGTCTGTCACGAAAACAATTATTGTTGGGTAG

Upstream 100 bases:

>100_bases
AGACTGGTCTAGTATTACTCTTAGGGCTGGGAATTTTTCTGATTGTGAAAGAAACCACCCAGTTAACTACCCAATTGGAG
TGGGTGCAGAAATTGTTCCA

Downstream 100 bases:

>100_bases
TACCGCAAGGCGGAAGTCATTAGTCATTAGTTACTTCCCCTATACCCTTACACCCCTATACCCCCAACCCCTTGATTTTA
CTTTTCATCAGTAAGTCACA

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSTTTRKSPSKKQRALEVLSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTE
LENLVRSTGFYRNKAKNIQAACRMIVSEYNSVVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGL
TKYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSVTKTIIVG

Sequences:

>Translated_223_residues
MSTTTRKSPSKKQRALEVLSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTE
LENLVRSTGFYRNKAKNIQAACRMIVSEYNSVVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGL
TKYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSVTKTIIVG
>Mature_222_residues
STTTRKSPSKKQRALEVLSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVTPALFSRFPDAPSLANADLTEL
ENLVRSTGFYRNKAKNIQAACRMIVSEYNSVVPNTMEQLLKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLT
KYPDPVHIEQDLMKLLPQPDWENWSIRLIYHGRAVCKARSPVCEACELADLCPSVTKTIIVG

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.2849162011173, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=36.5853658536585, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789331, Length=156, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=33.5164835164835, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=198, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=29.7752808988764, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550361, Length=182, Percent_Identity=34.0659340659341, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24753; Mature: 24622

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTTRKSPSKKQRALEVLSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVT
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
PALFSRFPDAPSLANADLTELENLVRSTGFYRNKAKNIQAACRMIVSEYNSVVPNTMEQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLTKYPDPVHIEQDLMKLLPQP
HHCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
DWENWSIRLIYHGRAVCKARSPVCEACELADLCPSVTKTIIVG
CCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STTTRKSPSKKQRALEVLSRLKRLYPDATCSLNYTTTVQLLVATILSAQCTDERVNLVT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCHHHHHH
PALFSRFPDAPSLANADLTELENLVRSTGFYRNKAKNIQAACRMIVSEYNSVVPNTMEQL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LKLPGVARKTANVVLAHAYGINAGVTVDTHVKRLSQRLGLTKYPDPVHIEQDLMKLLPQP
HHCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
DWENWSIRLIYHGRAVCKARSPVCEACELADLCPSVTKTIIVG
CCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]