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The map label for this gene is opuCB [H]

Identifier: 75676525

GI number: 75676525

Start: 2559103

End: 2560674

Strand: Direct

Name: opuCB [H]

Synonym: Nwi_2340

Alternate gene names: 75676525

Gene position: 2559103-2560674 (Clockwise)

Preceding gene: 75676524

Following gene: 75676530

Centisome position: 75.22

GC content: 63.04

Gene sequence:

>1572_bases
ATGAGCCTGCTGTTCGATCCGCGCTGGAGCGAAGCACTCGCTCACCTGCCGGACTATCTCGGCAACCACGTCCGCGTCAG
CCTAACGGCGCTGGCGCTGGGCCTCGCGATCAGCTTCCCGCTGGCGCTGACCGCCCGCAACCGGCCGATGCTGCGCAGCG
CGCTTCTCGGTTTTGCAAGCATCGTACAAACCATGCCGGGGCTGGCGCTGCTGGCGTTGTTCTATCCGCTCCTCCTGGCG
CTGGCGATTATGACATCGCAATGGTTCGGCTTCGGCTTCTCCGCGTTCGGATTCCTGCCTTCGGTGCTGGCGCTGGCGCT
CTATTCGATGCTTCCGGTGCTGCGCAACACCATCACCGGCCTTCAGGGGATCGATCCCGCATTGACGGAAGCGGCTAGAG
GGGTCGGCATGACGGCGCGGCAATCCCTCACCATGGTCGAGCTTCCGCTGGCGCTGCCGGTCATCATGGCGGGCATCCGC
ACCGCGGCGGTCTGGGTGATCGGCACCGCGACGCTCTCCACGCCCATCGGCCAGACCAGTCTCGGCAACTACATCTTCGC
CGGGCTGCAAACCCTGAACTGGGTGTTCGTGCTGTTCGGCTGCATCGCCGCGGCGGCCCTCGCGCTGGTCGTGGATCAGT
TGCTGGCGCTGATCGAATCCGGCTTGCGGATGCGCAGGCGCATCCGCGTGTCAACCGGTATTGTCGGCATCGCAATCCTG
GTTGCGGCGACATTGGCGCCCTCTCTGACCCGCGCGACATCCACCACGATCGTCGGCGCCAAAACCTTCACCGAACAATA
TGTGCTCGCGGCGCTGATCTCGCACCGGCTGCAAGCCGCGGGTCTTCCGGCGACGACCCGCGAGGGGCTCGGCTCCAACG
TCATTTTCGACGCGCTGGCGAACGGCGACATCGATGTCTACGTCGATTATTCCGGTACGCTGTGGGCCAATCAGTTCCAT
AGGACAGACCTGAGGCCGCGCGAGGAATTACTCACCGAGCTTGAGACGATACTGGCGCGGCGGAATATTACGCTGCTTGG
CGAACTCGGTTTTGAGAATGCCTACGCGCTGCTCATGCCAAAAGCGCGGGCCGACGCGCTGGGCATTCATACGATCTCCG
ATCTCGCCGCCCATGCCGGCAACATGTCGATCGCCGGCGACTACGAATTCTTCGCCCGGCCTGAATGGACGGCTCTGAAG
AAGGCTTACGGATTATCGTTTCGCGAGCGGCGACAGATGCAGTCGGATTTCATGTATGCCGCCGTGGCGTCCGGTGAAGT
CGACGTGATCGCGGGCTACACCAGCGACGGCCTGATCGCGAAATACGGCCTGACCGTGCTTGACGACACCAGACACGCAA
TTCCACCCTATGACGCGATTGTTCTGCTGGCGCCGCGGCGCGCCAATGACGCCAAGCTGCGCGCCGCGCTTCAGCCCCTG
CTTGGCAGGATCGACATCGCCATCATGCGGGAGGCCAACTTCCGCGCCAGCGACAGTGATACGGACAACTCACCGGCAAC
CGTGGCGCGGTGGTTGTGGACGAAGATCAATGAGAAGAAGATCAATGAGTAA

Upstream 100 bases:

>100_bases
AACTGTCCCGTATCGACAGCCCCTATGTCGGCGAACTGCTCGGCACGCCACGCCGCCAGGCCGAGCGACTTCAGGCCGCG
CTCTCGCAGAACATCGCGTC

Downstream 100 bases:

>100_bases
TAGGATGGTGATCTAACAGCGCGGGTGGGCACGGCGCTTCTTCCTCCCCTCCCCCTTGCGGGAGAGGGTGGCTTCGGACG
TAGTGACAAACCGGGTGAGG

Product: substrate-binding region and inner membrane component of ABC-type glycine betaine transport system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFASIVQTMPGLALLALFYPLLLA
LAIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITGLQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAIL
VAATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALANGDIDVYVDYSGTLWANQFH
RTDLRPREELLTELETILARRNITLLGELGFENAYALLMPKARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALK
KAYGLSFRERRQMQSDFMYAAVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPL
LGRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE

Sequences:

>Translated_523_residues
MSLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFASIVQTMPGLALLALFYPLLLA
LAIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITGLQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIR
TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAIL
VAATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALANGDIDVYVDYSGTLWANQFH
RTDLRPREELLTELETILARRNITLLGELGFENAYALLMPKARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALK
KAYGLSFRERRQMQSDFMYAAVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPL
LGRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE
>Mature_522_residues
SLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFASIVQTMPGLALLALFYPLLLAL
AIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITGLQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIRT
AAVWVIGTATLSTPIGQTSLGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAILV
AATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALANGDIDVYVDYSGTLWANQFHR
TDLRPREELLTELETILARRNITLLGELGFENAYALLMPKARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALKK
AYGLSFRERRQMQSDFMYAAVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPLL
GRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788449, Length=182, Percent_Identity=37.3626373626374, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788451, Length=211, Percent_Identity=32.2274881516588, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789033, Length=209, Percent_Identity=26.7942583732057, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56575; Mature: 56443

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFAS
CCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
IVQTMPGLALLALFYPLLLALAIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCHH
LGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALA
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHC
NGDIDVYVDYSGTLWANQFHRTDLRPREELLTELETILARRNITLLGELGFENAYALLMP
CCCEEEEEECCCCEECCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEC
KARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALKKAYGLSFRERRQMQSDFMYA
CCCCCHHCHHHHHHHHHHCCCCEECCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHEE
AVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPL
EECCCCEEEEEECCCCCCCHHCCCEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LGRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE
HHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
SLLFDPRWSEALAHLPDYLGNHVRVSLTALALGLAISFPLALTARNRPMLRSALLGFAS
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHH
IVQTMPGLALLALFYPLLLALAIMTSQWFGFGFSAFGFLPSVLALALYSMLPVLRNTITG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LQGIDPALTEAARGVGMTARQSLTMVELPLALPVIMAGIRTAAVWVIGTATLSTPIGQTS
CCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCCCCHH
LGNYIFAGLQTLNWVFVLFGCIAAAALALVVDQLLALIESGLRMRRRIRVSTGIVGIAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAATLAPSLTRATSTTIVGAKTFTEQYVLAALISHRLQAAGLPATTREGLGSNVIFDALA
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHC
NGDIDVYVDYSGTLWANQFHRTDLRPREELLTELETILARRNITLLGELGFENAYALLMP
CCCEEEEEECCCCEECCHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEC
KARADALGIHTISDLAAHAGNMSIAGDYEFFARPEWTALKKAYGLSFRERRQMQSDFMYA
CCCCCHHCHHHHHHHHHHCCCCEECCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHEE
AVASGEVDVIAGYTSDGLIAKYGLTVLDDTRHAIPPYDAIVLLAPRRANDAKLRAALQPL
EECCCCEEEEEECCCCCCCHHCCCEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LGRIDIAIMREANFRASDSDTDNSPATVARWLWTKINEKKINE
HHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]