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The map label for this gene is pcpB [H]

Identifier: 75676512

GI number: 75676512

Start: 2545486

End: 2546730

Strand: Direct

Name: pcpB [H]

Synonym: Nwi_2327

Alternate gene names: 75676512

Gene position: 2545486-2546730 (Clockwise)

Preceding gene: 75676511

Following gene: 75676513

Centisome position: 74.82

GC content: 63.86

Gene sequence:

>1245_bases
ATGACTTATCGAACCTGCTGCATTGTCGGCGCCGGCCCGGCAGGCCTCATGTTGGGTCTGCTGCTTGCACGCGCGGGCAT
CGACGTGACCGTGCTGGAGAAGCACGGCGATTTCCTGCGCGACTTTCGCGGCGATACGGTCCATCCTTCCACCCTCGAAG
TGATGCATGAACTCGGACTGCTTGAGGGCCTCCTGAAGCTGCCGCACACGCAGGCTCCGAGACTGCACGCCGAGATCGAC
GGCAAGGACATCACCATCGCGGACTTCTCGCGCCTGCCGACGAAGTGTCGTTTCATCGCGTTCATGCCGCAATGGGATTT
TCTCGACTATGTCGCGCGGGAAGCGGCCAGGTTTACGAACTTCAGGCTGATCATGCAGGCGGAGGTGACGGCGCTTATCG
AGGAGGACGGCCGGATCGCCGGAGTTCGGGCCCTGACACCAGGCGGCGATCTGGACATCAGAACCGCGCTGGTCGTCGGA
GCCGACGGGCGTAATTCGATCGTGCGGGAGAAGGCGGGCCTGCAGATCGAGCGCTTCGGAAGCCAGCGGGACGTGCTGTG
GATGAAGCTCTCACACATGCCCGACGACCCACCCTACGTCATGGGCCATGGCGGACCACGGCAGGGATTCGTCATGATCG
ACCGTGGCGATTATTGGCAATGCGGCTACGTCGTCCGGAAGGGCTCGTTCGATGACGTAAAGGCCACGGGTCTTGATGCA
TTCCGCGCAGCGGTCGCTTCGATCTCGCCGCTGTCGCCCGAACGCCTAAGCGAGGTGCGGTCGTGGGATGATGTCCATGT
GCTGAGCATACGCATTGACCGCTTGAAGAACTGGTGGCGGCCGGGCCTTCTCTGCATCGGTGATGCCGCCCATGCGATGT
CGCCGATCGGCGGCTTCGGCGTGAACCTTGCTATTCAGGATGCGGTCGCCGCCGCGAACATGCTCGCCGATCCGCTACGC
GACGGCGGACTGGCGGACAAGCATTTGGCCGCGGTCGAGGCGCGACGGTCCTTCCCGACGAAGGCGACGCAGAAAGTTCA
GCTGATGATGCAGGGCGCCCGCCGCAAGCGTGAAGCAGCGGATAGCGGACGACGGGACGGGCCGCCCTCCTTCATGCGGC
GCATGGCCCGATGGCCAATCCTTGCTCATCTGTCAGGCCGCCTCGTAGGTCTTGGCTTCAGGACCGAACATGTGCGCGAG
CGAGCGCCGCGTGTCACGCGCGTCAGGGAAAACGCGCCGGCATGA

Upstream 100 bases:

>100_bases
CCGGGGCGCCTGCGGGGTTGCATTCTGCGGCAGCAAATGATCGGTATGGTAAGCCCTCTCCGGAAAGATGCGGCCGAGCT
TGCAAGGAGATGCCGGAGAA

Downstream 100 bases:

>100_bases
CAGCCCATCGCGCCATCGCGCGTGGGCGCTCGCGGCGACGGGACGCACAGCCTGACCATTCAGATAGACCCCCTTTCAGA
TAGATCCCGCCGCAGTCACG

Product: hypothetical protein

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGLLEGLLKLPHTQAPRLHAEID
GKDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTNFRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVG
ADGRNSIVREKAGLQIERFGSQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDA
FRAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFGVNLAIQDAVAAANMLADPLR
DGGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAADSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRE
RAPRVTRVRENAPA

Sequences:

>Translated_414_residues
MTYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGLLEGLLKLPHTQAPRLHAEID
GKDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTNFRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVG
ADGRNSIVREKAGLQIERFGSQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDA
FRAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFGVNLAIQDAVAAANMLADPLR
DGGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAADSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRE
RAPRVTRVRENAPA
>Mature_413_residues
TYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGLLEGLLKLPHTQAPRLHAEIDG
KDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTNFRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVGA
DGRNSIVREKAGLQIERFGSQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDAF
RAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFGVNLAIQDAVAAANMLADPLRD
GGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAADSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRER
APRVTRVRENAPA

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=328, Percent_Identity=25, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 45797; Mature: 45665

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGL
CCEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHH
LEGLLKLPHTQAPRLHAEIDGKDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTN
HHHHHHCCCCCCCCEEEEECCCEEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHC
FRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVGADGRNSIVREKAGLQIERFG
EEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHCC
SQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDA
CCCCEEEEEECCCCCCCCEEECCCCCCCCEEEEECCCCEEECEEEECCCCCCHHHCCHHH
FRAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFG
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHCCCCCEEEECCHHHHHCCCCCCC
VNLAIQDAVAAANMLADPLRDGGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAA
EEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRERAPRVTRVRENAPA
HCCCCCCCHHHHHHHHHCCEEEECCCCEEEECCCHHHHHHHCCHHHHHHCCCCC
>Mature Secondary Structure 
TYRTCCIVGAGPAGLMLGLLLARAGIDVTVLEKHGDFLRDFRGDTVHPSTLEVMHELGL
CEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHH
LEGLLKLPHTQAPRLHAEIDGKDITIADFSRLPTKCRFIAFMPQWDFLDYVAREAARFTN
HHHHHHCCCCCCCCEEEEECCCEEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHC
FRLIMQAEVTALIEEDGRIAGVRALTPGGDLDIRTALVVGADGRNSIVREKAGLQIERFG
EEEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHCC
SQRDVLWMKLSHMPDDPPYVMGHGGPRQGFVMIDRGDYWQCGYVVRKGSFDDVKATGLDA
CCCCEEEEEECCCCCCCCEEECCCCCCCCEEEEECCCCEEECEEEECCCCCCHHHCCHHH
FRAAVASISPLSPERLSEVRSWDDVHVLSIRIDRLKNWWRPGLLCIGDAAHAMSPIGGFG
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHCCCCCEEEECCHHHHHCCCCCCC
VNLAIQDAVAAANMLADPLRDGGLADKHLAAVEARRSFPTKATQKVQLMMQGARRKREAA
EEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DSGRRDGPPSFMRRMARWPILAHLSGRLVGLGFRTEHVRERAPRVTRVRENAPA
HCCCCCCCHHHHHHHHHCCEEEECCCCEEEECCCHHHHHHHCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]