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The map label for this gene is 75676443

Identifier: 75676443

GI number: 75676443

Start: 2459113

End: 2460357

Strand: Direct

Name: 75676443

Synonym: Nwi_2258

Alternate gene names: NA

Gene position: 2459113-2460357 (Clockwise)

Preceding gene: 75676437

Following gene: 75676444

Centisome position: 72.28

GC content: 62.25

Gene sequence:

>1245_bases
ATGAACCTGCAACGCAAGCTGGAAATCCTGGCTGACGCCGCCAAGTACGACGCTTCCTGCGCCTCCAGCGGCACGGAACT
GCGGGACAGCCGCGACGGCAAGGGTCTCGGCTCGACCGCTCCCGGCATGGGCATCTGCCATTCCTATGCGCCGGACGGAC
GCTGCATTTCACTGCTGAAGGTGCTACTGACCAATGCCTGCAACTACGACTGCCTCTACTGCGTCAACCGGGCATCGTCG
AATGTGCCGCGCGCCCGCTTCACAATCGACGAAGTGGTCAGGCTGACCATCGACTTCTATCGCCGAAACTACATCGAGGG
GCTGTTTCTCTCTTCGGGCATCATCCGGAGCGCGGACTATACCATGGAGCAGGTGGTCGCTGTCGCGCGCAAACTACGCG
AGGAACATCGCTTTCGCGGCTACATCCATCTCAAGACCATTCCCGACGCGAATGATGCCTTGATCACGGAGGCCGGCAAA
TATGCCGATCGCCTCTCCATCAACATCGAGATGCCGGAGGAGGACAGCCTCGCGAAGCTTGCGCCCGAGAAGGATGCGCG
CGCCATCCGCCGCACCATGGGACGATTGCGGCTGAAGCTGGATGAAGCGAAGGAGGCAAGGAAGGCCGACGCCACAGCGC
GCGTGAGAGCGCCACGCTTCGCGCCGGCCGGCCAGACGACGCAGATGATCGTCGGTGCCGATGCCGCATCGGATCAAACG
ATCTTGAGCACAAGCGCCAATCTCTACGGCTCCTACCGCCTCAAGCGCGTCTATTACTCGGCCTTCAGCCCGATCCCCGA
CGCAAGCCGCGCCCTGCCCTTGAGCGCTCCGCCTCTCATGCGTGAACATCGGCTGTATCAGGCCGATTGGCTGATGCGTT
TCTATGGCTTTGACATCAGCGAGATCGTCGATGCCACGAACCCGATGCTGCCGCTCGACATCGATCCGAAACTCGCCTGG
GCGCTGCGCCACCGCGATCGCTTTCCGCTCGACATCAACCGCGCCAGCCGGGAAGAACTGCTACGGGTGCCGGGTTTTGG
AACCAAAGCCGTGGACCGCATTCTCTCCGCGCGACGTTACACCTCGATCCGCGCCGCGGACCTTTCGCGCCTGCACGTCC
CCCGAAACAAGGCGCTGCCCTTCATCGTTCTTGCCGACCACCGGCAGTCGCCGAAAGCCCTGGAGAGCATGACGCTGGCG
GAGCAGTTGAAGCCGAAAGCCGTGCAACTGGGGTTCGGATTTTGA

Upstream 100 bases:

>100_bases
GCCCGCCGTGGCCAAGAGTCAACCGGGACCTTTCCGGCGACGCTTTCGCCCGGGGGATCGCCAACCTTGTTTTGGTTGTG
GCGCCAAAGGATTGGAAAGC

Downstream 100 bases:

>100_bases
CCCGCCACATCATCCTCGACGGCGAAACCGATTTCGACGGTTGGCGCCGCGCCGCCCGCGCGCTCGCGCTCAACAATGCC
GCCCCGCACGATGTAGTCTG

Product: Elongator protein 3/MiaB/NifB

Products: NA

Alternate protein names: Radical SAM Domain Protein; Radical SAM Family Protein; Elongator /MiaB/NifB; Radical Sam Domain Protein; Radical SAM; Radical SAM Superfamily Protein; Biotin Synthase Related Domain-Containing Protein; Biotin Synthase Related Domain Containing Protein; Elongator Protein; Radical SAM Methylase Protein; Helix-Hairpin-Helix Motif; Radical SAM Protein

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLKVLLTNACNYDCLYCVNRASS
NVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADYTMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGK
YADRLSINIEMPEEDSLAKLAPEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQT
ILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDISEIVDATNPMLPLDIDPKLAW
ALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRYTSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLA
EQLKPKAVQLGFGF

Sequences:

>Translated_414_residues
MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLKVLLTNACNYDCLYCVNRASS
NVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADYTMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGK
YADRLSINIEMPEEDSLAKLAPEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQT
ILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDISEIVDATNPMLPLDIDPKLAW
ALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRYTSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLA
EQLKPKAVQLGFGF
>Mature_414_residues
MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLKVLLTNACNYDCLYCVNRASS
NVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADYTMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGK
YADRLSINIEMPEEDSLAKLAPEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQT
ILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDISEIVDATNPMLPLDIDPKLAW
ALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRYTSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLA
EQLKPKAVQLGFGF

Specific function: Unknown

COG id: COG4277

COG function: function code R; Predicted DNA-binding protein with the Helix-hairpin-helix motif

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46410; Mature: 46410

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00260 GLUCAGON

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLK
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHH
VLLTNACNYDCLYCVNRASSNVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADY
HHHHCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
TMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGKYADRLSINIEMPEEDSLAKL
HHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHC
APEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQT
CCCHHHHHHHHHHHHHEEHHHHHHHHHHCCCHHEECCCCCCCCCCCEEEEEECCCCCCCE
ILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDIS
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
EIVDATNPMLPLDIDPKLAWALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRY
HHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
TSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLAEQLKPKAVQLGFGF
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MNLQRKLEILADAAKYDASCASSGTELRDSRDGKGLGSTAPGMGICHSYAPDGRCISLLK
CCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHH
VLLTNACNYDCLYCVNRASSNVPRARFTIDEVVRLTIDFYRRNYIEGLFLSSGIIRSADY
HHHHCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
TMEQVVAVARKLREEHRFRGYIHLKTIPDANDALITEAGKYADRLSINIEMPEEDSLAKL
HHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHCCCCCEEEEEEEECCCCCCHHHC
APEKDARAIRRTMGRLRLKLDEAKEARKADATARVRAPRFAPAGQTTQMIVGADAASDQT
CCCHHHHHHHHHHHHHEEHHHHHHHHHHCCCHHEECCCCCCCCCCCEEEEEECCCCCCCE
ILSTSANLYGSYRLKRVYYSAFSPIPDASRALPLSAPPLMREHRLYQADWLMRFYGFDIS
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
EIVDATNPMLPLDIDPKLAWALRHRDRFPLDINRASREELLRVPGFGTKAVDRILSARRY
HHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHH
TSIRAADLSRLHVPRNKALPFIVLADHRQSPKALESMTLAEQLKPKAVQLGFGF
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA