BacMap

Definition	Nitrobacter winogradskyi Nb-255, complete genome.
Accession	NC_007406
Length	3,402,093

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The map label for this gene is fadB1 [H]

Identifier: 75676437

GI number: 75676437

Start: 2448842

End: 2449621

Strand: Direct

Name: fadB1 [H]

Synonym: Nwi_2252

Alternate gene names: 75676437

Gene position: 2448842-2449621 (Clockwise)

Preceding gene: 75676436

Following gene: 75676443

Centisome position: 71.98

GC content: 63.97

Gene sequence:

>780_bases
ATGCGCACTTTCGAGCACATCATCATCGAGAGCAAGGACCAGGTCGGCGTCATCCGATTGAACCGCCCGAAAATGCTCAA
CGCGCTGTCTTTCGCGGTGCTTCGCGAGATCGCAGCGGCGGTCGACGGGTTCGAGGCCGACGACGGAATCGGATGCATCC
TGATCACCGGCAACGAGAAGGCATTCGCCGCCGGAGCCGACATCAAGGAGATGCAGCCGAAAAGCTTCATCGATATGCTC
AACGAGGACTTCAAGGCCATCGGCGGCGACCGTATCGCCACCTGCCGCAAGCCGACGATCGCGGCGGTCAGCGGCTACGC
GCTCGGCGGCGGCTGCGAACTGGCCATGATGTGCGACATCATCATCGCCTCCGACAACGCGACATTCGGCCAACCCGAAA
TCACCCTCGGCACCATTCCCGGCATCGGCGGCACGCAGCGCCTGACCCGCGCCATCGGCAAGGCCAAGGCGATGGACCTC
TGCCTCACCGGGCGGATGATGGACGCAGCCGAGGCGGAGCGTTCGGGCCTCGTCAGTCGCGTCGTGCCCGTCGACAAGCT
GATGGACGAGGCGATGGCTGCCGCGAAAAAAATCGCGTCGATGTCGCGTCCAGCCGCGGCGATGGCGAAGGACGCCGTGA
ACCGCGCCTTCGAAACCACGCTGTCGGAAGGCATGAACGTCGAGCGCGACCTGTTTCGTGCGACCTTCGCGCTGGAGGAT
CGCGCCGAAGGCATGGCGGCGTTCATCGAGAAGCGCAAGCCGACCAACAAGAACCGATGA

Upstream 100 bases:

>100_bases
GGACACCGGTTCGCGTAAAGAAAACGCGTCAGATCAAAGTAAGAGCCCGCTTCTGGTTCTACCAGAAGCGGAAAGGCTCT
ATCACAAAGCGGGACCGACC

Downstream 100 bases:

>100_bases
CCTATCGTTCTCGCTCAGCGCGCGAGGCGACTGCGGCACCGTGTTTTACTTCCCGCTCTCCTTCAGCGCGCCATTGAGCA
GCGCACGGTAGAGCCTGTCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEKAFAAGADIKEMQPKSFIDML
NEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDIIIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDL
CLTGRMMDAAEAERSGLVSRVVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALED
RAEGMAAFIEKRKPTNKNR

Sequences:

>Translated_259_residues
MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEKAFAAGADIKEMQPKSFIDML
NEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDIIIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDL
CLTGRMMDAAEAERSGLVSRVVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALED
RAEGMAAFIEKRKPTNKNR
>Mature_259_residues
MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEKAFAAGADIKEMQPKSFIDML
NEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDIIIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDL
CLTGRMMDAAEAERSGLVSRVVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALED
RAEGMAAFIEKRKPTNKNR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=54.6153846153846, Blast_Score=278, Evalue=2e-75,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=31.6602316602317, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=37.8378378378378, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI68989263, Length=189, Percent_Identity=38.0952380952381, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI20127408, Length=192, Percent_Identity=35.4166666666667, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI37594471, Length=287, Percent_Identity=29.616724738676, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI37594469, Length=287, Percent_Identity=29.616724738676, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI70995211, Length=273, Percent_Identity=30.4029304029304, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI150378541, Length=242, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=29.7297297297297, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=28.3783783783784, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=28.3783783783784, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=25.2873563218391, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221307494, Length=207, Percent_Identity=29.951690821256, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=27.4881516587678, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=27.4881516587678, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI213417737, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=242, Percent_Identity=26.0330578512397, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=43.010752688172, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI62530384, Length=244, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI116235487, Length=228, Percent_Identity=26.7543859649123, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=43.3070866141732, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=35.8778625954198, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=36.8, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=34.9019607843137, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=29.9625468164794, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=31.7948717948718, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=33.1683168316832, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=52.4193548387097, Blast_Score=259, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=49.1735537190083, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=31.8777292576419, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=31.9066147859922, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=280, Percent_Identity=27.5, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=33.5164835164835, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=28.695652173913, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=279, Percent_Identity=27.9569892473118, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=279, Percent_Identity=27.9569892473118, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=181, Percent_Identity=34.8066298342541, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=181, Percent_Identity=34.8066298342541, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=181, Percent_Identity=34.8066298342541, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=28.6407766990291, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=243, Percent_Identity=26.3374485596708, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=216, Percent_Identity=28.7037037037037, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=55.1587301587302, Blast_Score=273, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=55.1587301587302, Blast_Score=273, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=35.7976653696498, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19920382, Length=265, Percent_Identity=31.6981132075472, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=26.0377358490566, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650670, Length=255, Percent_Identity=30.9803921568627, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=29.0196078431373, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24654903, Length=253, Percent_Identity=22.9249011857708, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28571729, Length=181, Percent_Identity=32.5966850828729, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=181, Percent_Identity=32.5966850828729, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583165, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921000, Length=184, Percent_Identity=33.1521739130435, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583077, Length=184, Percent_Identity=33.1521739130435, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583079, Length=184, Percent_Identity=33.1521739130435, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27769; Mature: 27769

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEK
CCCHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AFAAGADIKEMQPKSFIDMLNEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDI
EEECCCCHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCEEEECCEEECCCCCEEEEEEE
IIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDLCLTGRMMDAAEAERSGLVSR
EEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHH
VVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALED
HCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RAEGMAAFIEKRKPTNKNR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRTFEHIIIESKDQVGVIRLNRPKMLNALSFAVLREIAAAVDGFEADDGIGCILITGNEK
CCCHHHHEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCC
AFAAGADIKEMQPKSFIDMLNEDFKAIGGDRIATCRKPTIAAVSGYALGGGCELAMMCDI
EEECCCCHHHCCHHHHHHHHHHHHHHCCCCCEECCCCCCEEEECCEEECCCCCEEEEEEE
IIASDNATFGQPEITLGTIPGIGGTQRLTRAIGKAKAMDLCLTGRMMDAAEAERSGLVSR
EEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHH
VVPVDKLMDEAMAAAKKIASMSRPAAAMAKDAVNRAFETTLSEGMNVERDLFRATFALED
HCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RAEGMAAFIEKRKPTNKNR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]