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The map label for this gene is mutB [H]

Identifier: 75676423

GI number: 75676423

Start: 2432853

End: 2435021

Strand: Direct

Name: mutB [H]

Synonym: Nwi_2238

Alternate gene names: 75676423

Gene position: 2432853-2435021 (Clockwise)

Preceding gene: 75676422

Following gene: 75676424

Centisome position: 71.51

GC content: 65.15

Gene sequence:

>2169_bases
ATGATGGCCCGCGTTCCGGATTTCGCCGACGTGGCGTTCGAGCCGGTCGCGCCTCCCGTCGCCGGCGGCTTCGAACGCTC
CCCGGAAGTCTGGCTGACCCCGGAAGGCATTCCGGTCAAGACCGCCTATTCGGAAGCCGATCTTGACGGGATCGACTTCC
TCGACACATGGCCCGGCATCGCGCCTTACCTGCGCGGCCCCTACCCGACCATGTATGTCAACCAGCCCTGGACCATCCGG
CAGTATGCGGGGTTCTCGACGGCGGAGGATTCCAACGCCTTCTATCGGCGTAACCTCGCCGCCGGCCAGAAAGGGCTGTC
GGTCGCCTTCGACCTCGCCACCCATCGCGGCTATGATTCCGATCACCCGCGCGTTTCGGGCGACGTCGGCATGGCCGGCG
TCGCGATCGATTCTATCTACGACATGCGCACGCTGTTCGCCGGCATTCCGCTCGACCGGATCAGCGTATCGATGACCATG
AACGGCGCGGTGCTCCCGATCCTCGCGCTGTTCGTCGCGGCGGCCGAGGAACAGGGCGTGCCGGCGGAAAAACTCTCGGG
CACCATTCAGAACGACATCCTCAAGGAGTTCATGGTGCGCAACACCTACATCTATCCGCCCGCGCCTTCGATGCGGATCG
TTTCCGACATCTTCGCCTACGCCTCGCGCAGGATGCCGAAGTTCAACTCGATTTCCGTTTCGGGCTACCACATGCAGGAG
GCCGGCGCGACGCAGGACCTGGAACTCGCCTATACGCTGGCCGACGGCGTCGAATATGTCCGCGCGGGCCTCAAGGCGGG
CCTGGACATCGATCGCTTCGCGCCGCGGCTGTCGTTCTTCTGGGCGACCGGCATGAACTTCTTCATGGAAGTCGCCAAGA
TGCGCGCCGCGCGGCTGCTATGGGCGAAGCTCATGAAGCCGTTCAATCCGAAGAATCCGCGTTCGCTCAGCCTGCGCACC
CACTGCCAGACTTCCGGCTGGTCGCTGACCGCGCAGGACGTTTTCAACAACGTGATCCGCACCGCTATCGAGGCGATGGC
GGCGACGCAAGGCCATACCCAATCGCTGCACACCAACGCGCTGGACGAGGCTCTGGCGCTGCCGACCGACTTCTCCGCCC
GCATCGCCCGCAACACCCAGCTTTTCCTGCAACAGGAGAGCGGCACCACGCGCATCATCGATCCCTGGGGCGGCTCGCAT
TACGTCGAACGGCTCACGCATGATCTCGCAATCAAGGCCTGGGGCCATATTCGCGAAGTCGAGGAACTCGGCGGCATGGC
CAGGGCGATCGAGGCCGGCGTGCCGAAGCTGCGCATCGAGGAAGCGGCGGCGCGGACGCAGGCCAGGATCGACGCCGGAG
CGCAGGTCGTGATCGGCGTCAACAGGTACAAGCCCGAGCATGAGACGCCGATCGACGTGCTCAGGATCGACAACTCGGCG
GTGCGAAGCCTGCAGATCGAGAAACTGAAACGATTGCGCGGCGAGCGCAGGCAGGAGGATGTCGATGCCGCGCTGGCGGC
GCTGACGCGCTCGGCCGGCGAAGGCAACGGCAACCTGCTGGCGCTCGCGATCGACGCCGCGCGCGCCAAGGCGACCGTCG
GCGAAATTTCCGACGCAATGGAGAAGGTGTTCGGCCGGCATCGCGCCGAGATCAGGTCGATCACCGGGGTCTACAAGCGC
GAGGTGTCCGCGATGTCCGACAAGGTGGAGCAGCTGCAACGGCTCATCGACGCCTTCGATGAGGCCGAGGGCCGCCGTCC
GCGAATTCTCGTCGCCAAGATCGGCCAGGACGGCCACGACCGCGGCCAGAAAGTGATCGCTTCCGCCTTCGCCGATATCG
GCTTCGACGTCGATATCGGACCGTTGTTCGCGACAGCGGAGGAAGCCGCGCGGCAGGCGATCGAGAACGACGTGCACATT
CTCGGCGTGTCCTCGCTCGCCGCCGCGCATCTGACCGCGGTGCCGGAACTGAAGGCAGCGCTGACAAGACATGGCCGCGA
CGACATCATGATCATCGTCGGCGGCGTGGTGCCGCCGCAGGACTACGATGCGCTTTATAAAGCCGGCGCGGAGGCGATCT
TTCCTCCCGGCACGGTGATCGCAGACGCCGCCGAAGACCTGATCCGCAAGCTCAGCGCGCGGCTCGGACACGGAACGACG
ATGGAGTAG

Upstream 100 bases:

>100_bases
GACCATCGTCAACCTGCCGAGCCACACTACACCCGAAAATTACGGACCCGATAAGATGGCGACCATCATCAGCCACCAGC
ACCCGGACCATGACTGATCG

Downstream 100 bases:

>100_bases
CACAGGCGTTCTTAAACTCTCCACAAATGACCATCATGCCCGCGCTTGTCGCGCGCATCCACGTCTCTATGATCCGGACT
CCAGGACGCGGATGGCCGGG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGIAPYLRGPYPTMYVNQPWTIR
QYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTM
NGAVLPILALFVAAAEEQGVPAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQE
AGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLLWAKLMKPFNPKNPRSLSLRT
HCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSH
YVERLTHDLAIKAWGHIREVEELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSA
VRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAMEKVFGRHRAEIRSITGVYKR
EVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHI
LGVSSLAAAHLTAVPELKAALTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTT
ME

Sequences:

>Translated_722_residues
MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGIAPYLRGPYPTMYVNQPWTIR
QYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTM
NGAVLPILALFVAAAEEQGVPAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQE
AGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLLWAKLMKPFNPKNPRSLSLRT
HCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSH
YVERLTHDLAIKAWGHIREVEELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSA
VRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAMEKVFGRHRAEIRSITGVYKR
EVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHI
LGVSSLAAAHLTAVPELKAALTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTT
ME
>Mature_722_residues
MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGIAPYLRGPYPTMYVNQPWTIR
QYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTM
NGAVLPILALFVAAAEEQGVPAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQE
AGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLLWAKLMKPFNPKNPRSLSLRT
HCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSH
YVERLTHDLAIKAWGHIREVEELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSA
VRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAMEKVFGRHRAEIRSITGVYKR
EVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHDRGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHI
LGVSSLAAAHLTAVPELKAALTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTT
ME

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=682, Percent_Identity=62.9032258064516, Blast_Score=909, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=686, Percent_Identity=60.2040816326531, Blast_Score=835, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=709, Percent_Identity=61.0719322990127, Blast_Score=894, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78838; Mature: 78838

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGI
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHCCCCC
APYLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDS
CHHHCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCC
DHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTMNGAVLPILALFVAAAEEQGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHHHHHHCCC
PAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQE
CHHHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEECC
AGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLL
CCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAKLMKPFNPKNPRSLSLRTHCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNA
HHHHHCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSHYVERLTHDLAIKAWGHIREV
HHHHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSA
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCHH
VRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHH
EKVFGRHRAEIRSITGVYKREVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
RGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHILGVSSLAAAHLTAVPELKAA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
LTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTT
HHHCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ME
CC
>Mature Secondary Structure
MMARVPDFADVAFEPVAPPVAGGFERSPEVWLTPEGIPVKTAYSEADLDGIDFLDTWPGI
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHCCCCC
APYLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGLSVAFDLATHRGYDS
CHHHCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHCCCCC
DHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDRISVSMTMNGAVLPILALFVAAAEEQGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCHHHHHHHHHHHHHHHCCC
PAEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIVSDIFAYASRRMPKFNSISVSGYHMQE
CHHHHCCHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEECC
AGATQDLELAYTLADGVEYVRAGLKAGLDIDRFAPRLSFFWATGMNFFMEVAKMRAARLL
CCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WAKLMKPFNPKNPRSLSLRTHCQTSGWSLTAQDVFNNVIRTAIEAMAATQGHTQSLHTNA
HHHHHCCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LDEALALPTDFSARIARNTQLFLQQESGTTRIIDPWGGSHYVERLTHDLAIKAWGHIREV
HHHHHCCCCCCHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EELGGMARAIEAGVPKLRIEEAAARTQARIDAGAQVVIGVNRYKPEHETPIDVLRIDNSA
HHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCHH
VRSLQIEKLKRLRGERRQEDVDAALAALTRSAGEGNGNLLALAIDAARAKATVGEISDAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHH
EKVFGRHRAEIRSITGVYKREVSAMSDKVEQLQRLIDAFDEAEGRRPRILVAKIGQDGHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
RGQKVIASAFADIGFDVDIGPLFATAEEAARQAIENDVHILGVSSLAAAHLTAVPELKAA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
LTRHGRDDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEDLIRKLSARLGHGTT
HHHCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
ME
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]